We narrowed to 8,589 results for: Set

1. pcDNA3.1 LIC 6A CARD8-FLAG E274R

   Plasmid

   #169950

   Purpose

   Mammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R)

   Depositor

   Hao Wu

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E274R

   Promoter

   CMV

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 LIC 6A CARD8-FLAG F370G

   Plasmid

   #169953

   Purpose

   Mammalian expression of CARD8-FLAG with a site III mutation (F370G)

   Depositor

   Hao Wu

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   F370G

   Promoter

   CMV

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. F-MED26

   Plasmid

   #15367

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Sato et al Mol Cell. 2004 Jun 4. 14(5):685-91.

   Insert

   MED26 (MED26 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pSicoR-eIF1a-GFP-RPL10a

   Plasmid

   #159890

   Purpose

   Expresses GFP-RPL10a for ribosomal immunoprecipitation

   Depositor

   James Ellis

   Article

   Rodrigues et al Cell Rep. 2020 Mar 24;30(12):4179-4196.e

   Insert

   RPL10a (RPL10A Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Promoter

   eIF1a

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)GαsL-260-NLuc

   Plasmid

   #207158

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes long transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAS (GNAS Human)
   

   Expression

   Mammalian

   Mutation

   NLuc inserted at position D(260)N

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV-opEBNA2-IRES-mCherry

   Plasmid

   #220355

   Purpose

   Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression)

   Depositor

   Klaus Rajewsky

   Article

   Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

   Insert

   opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   codon-optimised for expression in mammalian cells

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pInducer20 CARD8-HA PAM E274R

   Plasmid

   #169984

   Purpose

   Tet/Dox-inducible mammalian expression of CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PAM, E274R

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENT-L1-Kozak-GAP43-ECFP-stop-L2

   Plasmid

   #186356

   Purpose

   Entry clone with ORF encoding plasma membrane-targeted GAP43-eCFP flanked by Gateway recombination sequences

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Insert

   Growth-associated protein-43 (Gap43 Mouse, Synthetic)
   

   Use

   Expression of a fluorescent membrane marker

   Tags

   ECFP

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. H14-MBP-SUMO-Cys-Linker-BAF_G47E

   Plasmid

   #101779

   Purpose

   Expresses His-MBP-tagged mutant BAF (G47E) in E.coli (SUMO-cleavable)

   Depositor

   Daniel Gerlich

   Article

   Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

   Insert

   BAF (BANF1) (BANF1 Human)
   

   Tags

   His14-MBP-SUMO

   Expression

   Bacterial

   Mutation

   G47E mutation

   Promoter

   T5

   Available Since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-sgRELA

    Plasmid

    #83944

    Purpose

    Lentiviral vector expressing an sgRNA targeting RELA NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgRELA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENT-L1-Kozak-Ensconsin-3XGFP-stop-L2

    Plasmid

    #186361

    Purpose

    Entry clone with ORF encoding the MT-binding domain of Ensconsin fused to 3 copies of GFP in C-terminal, flanked by Gateway recombination sequences.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    Human Ensconsin (MAP7 Human, Synthetic)
    

    Use

    Expression of a fluorescent microtubule marker

    Tags

    3xEGFP

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. H14-MBP-SUMO-FLAG-LEM

    Plasmid

    #101778

    Purpose

    Expresses His-MBP-tagged mutant LEM-domain (FLAG-tagged) in E.coli (SUMO-cleavable)

    Depositor

    Daniel Gerlich

    Article

    Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

    Insert

    MAN1 (LEMD3 Human)
    

    Tags

    His14-MBP-SUMO-FLAG

    Expression

    Bacterial

    Mutation

    amino acids 6-51 of human MAN1 (LEM domain)

    Promoter

    T5

    Available Since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. F-MED28

    Plasmid

    #15368

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al Mol Cell. 2004 Jun 4. 14(5):685-91.

    Insert

    MED28 (MED28 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pLenti-sgCCR5

    Plasmid

    #83930

    Purpose

    Lentiviral vector expressing an sgRNA targeting CCR5 NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgCCR5
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCGT-HCF-1-C600

    Plasmid

    #99364

    Purpose

    C-term of HCFC1 protein

    Depositor

    Winship Herr

    Article

    Wilson et al Mol Cell Biol. 2000 Sep;20(18):6721-30.

    Insert

    HCF-1 C-term 1436-2035 (HCFC1 Human)
    

    Tags

    2x tandem T7 tag and C-myc

    Expression

    Mammalian

    Mutation

    Deletion of HCFC1 cDNA

    Promoter

    CMV

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. H14-MBP-SUMO-Cys-Linker-BAF_L58R

    Plasmid

    #101780

    Purpose

    Expresses His-MBP-tagged mutant BAF (L58R) in E.coli (SUMO-cleavable)

    Depositor

    Daniel Gerlich

    Article

    Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

    Insert

    BAF (BANF1) (BANF1 Human)
    

    Tags

    His14-MBP-SUMO

    Expression

    Bacterial

    Mutation

    L58R mutation

    Promoter

    T5

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1(+)GαsS-246-Nluc

    Plasmid

    #207157

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes short transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2

    Depositor

    Dominique Schols, Tom Van Loy

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNAS (GNAS Human)
    

    Expression

    Mammalian

    Mutation

    NLuc inserted at position D(246)N

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST-pSP172BSSPE-R3-ccdB/CmR-R4-bpFOG-B5::B1-NLSLacZ-B2

    Plasmid

    #186414

    Purpose

    AttR3/R4 destination vector for ascidian electroporation with AttB1/B2 recombined NLSLacZ under control of AttB4/B5-recombined pFOG ectodermal regulatory sequence.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    lacZ (lacZ E. coli)
    

    Use

    Gateway destination vector

    Tags

    Nuclear localization signal

    Promoter

    Basal promoter of the FOG gene

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F370G

    Plasmid

    #169960

    Purpose

    Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Tags

    FLAG and Ub

    Expression

    Mammalian

    Mutation

    F370G

    Promoter

    CMV

    Available Since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pInducer20 CARD8-HA PAM L368G

    Plasmid

    #169985

    Purpose

    Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (L368G) with sgRNA binding site mutations

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    PAM, L368G

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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