We narrowed to 6,084 results for: nls

1. pLenti-FNLSHF1-P2A-Puro

   Plasmid

   #136901

   Purpose

   Lentivirus for constitutive expression of FNLSHF1 in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   FNLSHF1
   

   Use

   Lentiviral

   Tags

   FLAG-NLS and NLS

   Expression

   Mutation

   R661A,Q695A,Q926A

   Promoter

   EF1s

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. EF1a-3NLS-Sox2TALE-GCN4

   Plasmid

   #120548

   Purpose

   Express 3xNLS-Sox2 TALE-GCN4 engineered to bind a site in the human SOX2 gene

   Depositor

   Yu Wang

   Article

   Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

   Insert

   NLS, Sox2 TALE, GCN4 (SOX2 Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. EF1a-3NLS-NGN2TALE-GCN4

   Plasmid

   #120547

   Purpose

   Express 3xNLS-NGN2 TALE-GCN4 engineered to bind a site in the human NGN2 ene

   Depositor

   Yu Wang

   Article

   Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

   Insert

   NLS, NGN2 TALE, GCN4 (NEUROG2 Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCSD_FRB-TALETS3-3xFLAG-2xNLS

   Plasmid

   #107306

   Purpose

   Expresses TALE-VEGFA-TS3 fused to FRB in mammalian cells

   Depositor

   Scot Wolfe

   Insert

   TALE_VEGFA-TS3
   

   Use

   CRISPR

   Tags

   3x Flag, 2xNLS and FRB

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPHR-EYFP-hGCN5-NLS

   Plasmid

   #103827

   Purpose

   Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   PHR-EYFP-hGCN5-NLS (CRY2, KAT2A Human, Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hGCN5: A566G (E189G), G585T (K195N), G726A (silen…

   Promoter

   CMV

   Available since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. DsRed-CPI-17-T38A-NLS

   Plasmid

   #100792

   Purpose

   Mammalian expression of Fluorescent CPI-17 neutral mutation nuclear

   Depositor

   Joseph Gordon

   Insert

   protein phosphatase 1 regulatory inhibitor subunit 14A (PPP1R14A Human)
   

   Use

   Tags

   DsRed

   Expression

   Mammalian

   Mutation

   T38A

   Promoter

   CMV

   Available since

   Nov. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. DsRed-CPI-17-T38E-NLS

   Plasmid

   #100790

   Purpose

   Mammalian expression of Fluorescent CPI-17 phospho mimetic nuclear

   Depositor

   Joseph Gordon

   Insert

   CPI-17-T38E-NLS (PPP1R14A Human)
   

   Use

   Tags

   DsRed

   Expression

   Mammalian

   Mutation

   T38E

   Promoter

   CMV

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEN_TTmiRc2 3XFLAG-EGFP-NLS

   Plasmid

   #87778

   Purpose

   Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   eGFP
   

   Use

   Tags

   3xFLAG and NLS

   Expression

   Mutation

   Promoter

   Available since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEN_TTmiRc2 3XFLAG-MKK7-EE-NLS

   Plasmid

   #87777

   Purpose

   Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   MKK7-EE
   

   Use

   Tags

   3xFLAG and NLS

   Expression

   Mutation

   Promoter

   Available since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEN_TTmiRc2 3XFLAG-MKK4-EE-NLS

    Plasmid

    #87776

    Purpose

    Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKK4 (MAP2K4 Human)
    

    Use

    Tags

    3xFLAG and NLS

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-myc-mCE NLS

    Plasmid

    #82465

    Purpose

    Expresses myc tag and mRNA capping enzyme without Nuclear Localization Sequence (NLS) from C terminus

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    Capping Enzyme (Rngtt Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    deleted amino acids Nuclear Localization Signal

    Promoter

    CMV

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBABE-3xFLAG-NLS-TPP1-OB

    Plasmid

    #79060

    Purpose

    Retroviral expression of 3xFLAG tagged, nuclear localized wild-type TPP1-OB folding domain

    Depositor

    Steven Artandi

    Article

    Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

    Insert

    TPP1 (TPP1 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Wild-type TPP-OB folding domain

    Promoter

    CMV

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pLPC-Flag-CHES1L-dC-NLS

    Plasmid

    #51840

    Purpose

    Flag-CHES1L mutant lacking the C-terminal, with remaining NLS

    Depositor

    Gerardo Ferbeyre

    Article

    Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

    Insert

    CHES1 (FOXN3 Human)
    

    Use

    Retroviral

    Tags

    Flag and NLS

    Expression

    Mutation

    contains CHES1 amino acids 1-205

    Promoter

    Available since

    July 17, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pLPC-Flag-CHES1LdFC-NLS

    Plasmid

    #51838

    Purpose

    Flag-CHES1L mutant lacking the forkhead binding site and C-terminal domain, with remaining NLS

    Depositor

    Gerardo Ferbeyre

    Article

    Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

    Insert

    CHES1 (FOXN3 Human)
    

    Use

    Retroviral

    Tags

    Flag and NLS

    Expression

    Mutation

    contains CHES1 amino acids 1-109

    Promoter

    Available since

    July 17, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS

    Plasmid

    #71396

    Purpose

    2Gi2R assay

    Depositor

    Fred Gage

    Article

    Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

    Inserts

    2xGFP:NES
    2xRFP:NLS
    

    Use

    Lentiviral

    Tags

    IRES

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha and IRES

    Available since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. SBE-NLS-mCherry PGK-rtTA3

    Plasmid

    #195332

    Purpose

    Lentiviral construct of TGFβ reporter (nuclear mCherry) and hPGK driven rtTA3

    Depositor

    Elaine Fuchs

    Article

    Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

    Inserts

    NLS-mCherry
    rtTA3
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and miniCMV

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pL6puro-pmRevErbA-Venus-NLS-Pest

    Plasmid

    #240105

    Purpose

    A lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein)

    Depositor

    Achim Kramer

    Article

    Gabriel et al bioRxiv 2025.06.30.661926

    Insert

    murine Nr1d1 promoter+Venus-NLS-Pest-Reporter (Nr1d1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-EFS-NLS-CasRx-U6-DR-sgRNA_LacZ-DR

    Plasmid

    #154003

    Purpose

    Vector expressed CasRx and sgRNA_LACZ for AAV package

    Depositor

    Hui Yang

    Article

    He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

    Insert

    CasRx
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. hGFAP-NLS-HA-Dre-P2A-BFP

    Plasmid

    #51277

    Purpose

    Co-expression of the site-specific recombinase Dre and BFP under the control of the hGFAP promoter

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    NLS-HA-Dre-P2A-BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hGFAP

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)

    Plasmid

    #196703

    Purpose

    Plasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

    Insert

    human RNaseH1 D210N
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D210N, catalytically inactive

    Promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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