We narrowed to 59,429 results for: sap

1. pYeDP60-hATP13A2-E343A

   Plasmid

   #171378

   Purpose

   A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888

   Depositor

   Peter Vangheluwe

   Article

   van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

   Insert

   human ATP13A2 variant 2 wild-type
   

   Use

   Tags

   BAD tag

   Expression

   Yeast

   Mutation

   E343A

   Promoter

   URA3

   Available since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX307/PTPN11

   Plasmid

   #140941

   Purpose

   expresses PTPN11

   Depositor

   Kimberly Stegmaier

   Article

   Cremer et al Cancer Discov. 2020 Feb;10(2):214-231. d

   Insert

   PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. PSD-95 scFv [K28/42]

   Plasmid

   #190488

   Purpose

   Mammalian Expression of PSD-95 scFV. Derived from hybridoma K28/42.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   PSD-95 (Homo sapiens) recombinant scFV (DLG4 Mouse)
   

   Use

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223_MAPK13_WT

   Plasmid

   #82231

   Purpose

   Gateway Donor vector containing MAPK13 , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   MAPK13 (MAPK13 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCB268

   Plasmid

   #53363

   Purpose

   pcDNA3.0-based plasmid encoding fDHFR-UbK48R-Aβ42 13myc under the control of T7 or CMV promoter for 35S-pulse-chase URT-based assays in rabbit reticulocyte extract.

   Depositor

   Alexander Varshavsky

   Article

   Brower et al Mol Cell. 2013 Apr 25;50(2):161-71. doi:

   Inserts

   DHFR (Dhfr Mouse)
   Ub
   Aβ42 (APP Human)
   

   Use

   Tags

   13xMyc, Flag, and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pDONR223_MAPK14_WT

   Plasmid

   #82298

   Purpose

   Gateway Donor vector containing MAPK14, part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   MAPK14 (MAPK14 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. PSD-95 scFv [K28/43]

   Plasmid

   #190489

   Purpose

   Mammalian Expression of PSD-95 scFV. Derived from hybridoma K28/43.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   PSD-95 (Homo sapiens) recombinant scFV (DLG4 Mouse)
   

   Use

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PSD-95 scFv [K28/38]

   Plasmid

   #190487

   Purpose

   Mammalian Expression of PSD-95 scFV. Derived from hybridoma K28/38.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   PSD-95 (Homo sapiens) recombinant scFV (DLG4 Mouse)
   

   Use

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-AICD-NLS-IRES-hrGFP

   Plasmid

   #107543

   Purpose

   AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Localisation Signal (NLS: CCAAAAAAGAAGAGAAAGGTA) at C-term end and IRES-mediated co-expression of hrGFP

   Depositor

   Hélène Marie

   Article

   Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

   Insert

   AICD-NLS (APP Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   CMV

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLX307/PTPN11 T73I

    Plasmid

    #140939

    Purpose

    expresses PTPN11 T73I

    Depositor

    Kimberly Stegmaier

    Article

    Cremer et al Cancer Discov. 2020 Feb;10(2):214-231. d

    Insert

    PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    T73I

    Promoter

    EF1a

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-AICD-NES-IRES-hrGFP

    Plasmid

    #107544

    Purpose

    AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Export Signal (NES: CTCCCTCCACTAGAGCGACTAACCTTA) at C-term end and IRES-mediated co-expression of hrGFP

    Depositor

    Hélène Marie

    Article

    Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

    Insert

    AICD-NES (APP Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    CMV

    Available since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX307/PTPN11 E76G

    Plasmid

    #140862

    Purpose

    expresses PTPN11 E76G

    Depositor

    Kimberly Stegmaier

    Article

    Cremer et al Cancer Discov. 2020 Feb;10(2):214-231. d

    Insert

    PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E76G

    Promoter

    EF1a

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHLsec hSOD1

    Plasmid

    #232481

    Purpose

    vector for transient transfection expressing human SOD1gene including flag-tag (DYKDDDDK) on the 3' end

    Depositor

    Lukas Trantirek

    Article

    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

    Insert

    Homo sapiens superoxide dismutase 1 (SOD1 Human)
    

    Use

    Tags

    DYKDDDDK (FLAG-tag)

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. OMM-short-RspA-CFAST

    Plasmid

    #233594

    Purpose

    Expression of RspA-CFAST on the outer mitochondrial membrane

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    TOM70 fragment-RspA-CFAST (TOMM70 RspA-CFAST from Rheinheimera sp A13L and TOM70 fragment from Homo sapiens, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1 FFSS-BACH1 C122E

    Plasmid

    #232274

    Purpose

    Expression of N-terminal tagged 2xFLAG-2xSTREP-BACH1 (H. sapiens) with Cys122 to Glu point mutation in human cell lines

    Depositor

    Michele Pagano

    Article

    Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

    Insert

    BACH1 (BACH1 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    changed cysteine 122 to glutamic acid

    Promoter

    CMV

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLV2.2-h7SKgRNA5(RUNX1(13))-hU6gRNA5(RUNX3(12))-PGKpuroBFP-W

    Plasmid

    #208569

    Purpose

    Lentiviral vector expressing gRNA targeting human RUNX1 and RUNX3

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    RUNX1(13), RUNX3(12) (RUNX1, RUNX3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKLV2.2-h7SKgRNA5(RUNX1(13))-hU6gRNA5(RUNX3(13))-PGKpuroBFP-W

    Plasmid

    #208570

    Purpose

    Lentiviral vector expressing gRNA targeting human RUNX1 and RUNX3

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    RUNX1(13), RUNX3(13) (RUNX1, RUNX3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CL20_mEGFP_MSH6_FBXO11

    Plasmid

    #205859

    Purpose

    Express mEGFP-tagged fusion protein, MSH6_FBXO11 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    MSH6_FBXO11 (MSH6, FBXO11 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CL20_mEGFP_JARID2_SYNCRIP

    Plasmid

    #205834

    Purpose

    Express mEGFP-tagged fusion protein, JARID2_SYNCRIP from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    JARID2_SYNCRIP (JARID2, SYNCRIP Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC19-POLBHR-eGFP

    Plasmid

    #176064

    Purpose

    Homology region +/- 800bp to the transcription start site of POLB, with EGFP inserted in-frame on the N-terminus of POLB, and a mutation in the PAM site used by POLBKO gRNA1 in POLB exon1

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    EGFP-PolB (POLB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    pUC with endogenous SapI mutated out, PAM mutatio…

    Promoter

    N/A

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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