We narrowed to 4,170 results for: spl

Showing: 1121 - 1140 of 4170 results

Results per page:

Sort results by:

pMito.iGABASnFR.mRuby3

Plasmid

#112179

Purpose

GABA Sensor

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR

Tags

mRuby3

Expression

Mammalian

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pU1_000

Plasmid

#186773

Purpose

* Engineered U1 snRNA plasmid for insertion of guide sequences.

Depositor

Eugene Yeo

Publication

Hatch et al Methods. 2022 Jun 25;205:140-148. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U1 Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TC1154

Plasmid

#164876

Purpose

Split GFP reporter plasmid expressing GFP (b11beta

Depositor

Tian Chi

Publication

Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

Insert

Split GFP(beta11)

Expression

Mammalian

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TC1541A

Plasmid

#164875

Purpose

Split GFP reporter expressing GFP(beta1-10) following C>U editing of the target site

Depositor

Tian Chi

Publication

Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

Insert

Split GFP(beta1-10)

Expression

Mammalian

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CaMKIV-dCT

Plasmid

#126422

Purpose

Plasmid expresses constitutively active form of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)

Depositor

Douglas Black

Publication

Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi

Insert

Calcium/calmodulin-dependent protein kinase type IV (CAMK4 Human)

Tags

FLAG

Expression

Mammalian

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pENTR-NLS-actin

Plasmid

#118380

Purpose

Gateway compatible donor vector with nuclear localization signal (NLS) attached to WT actin.

Depositor

Maria Vartiainen

Publication

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Mutation

NLS

Available since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiFC-flag-hCaspase 2 CARD-VN173

Plasmid

#49260

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Publication

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 CARD (CASP2 Human)

Tags

Flag and split Venus N

Expression

Mammalian

Mutation

delete aa 123-435

Promoter

CMV

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
pEBB-II-I-GFP beta isoform

Plasmid

#22147

Depositor

Ananda Roy

Publication

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GFP

Expression

Mammalian

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
(112) pcDNA3.1-GFP-Flag-Nup62-EPEA

Plasmid

#168101

Purpose

GFP and Flag tagged Nup62 with EPEA nanobody tag

Depositor

Christina Woo

Publication

Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

Insert

GFP-Flag-Nup62-EPEA

Tags

EPEA, Flag, and GFP

Expression

Mammalian

Promoter

T7/CMV

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
OPA1-isoform6

Plasmid

#70178

Purpose

Mammalian expression of hsOPA1 isoform 6

Depositor

David Chan

Publication

Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

Insert

hsOPA1 isoform 6 (OPA1 Human)

Use

Retroviral

Expression

Mammalian

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only
pAC1813_pCR8-gSMN2-DN1

Plasmid

#118646

Purpose

Transient transfection; Expressed SMN2-DN1 CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Publication

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gSMN2-DN1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pAC1806_pmax-FKBP-dCasRx

Plasmid

#118639

Purpose

Transient transfection; Expresses FKBP-dCasRx; CAGGS promoter

Depositor

Albert Cheng

Publication

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

FKBP-dCasRx

Use

CRISPR

Expression

Mammalian

Promoter

CAGGS

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4-His-Max-A-PQBP1

Plasmid

#25802

Depositor

Marius Sudol

Publication

Tapia et al J Biol Chem. 2010 Jun 18;285(25):19391-4

Insert

PQBP1 (PQBP1 Human)

Tags

His and Xpress

Expression

Mammalian

Available since

July 21, 2010

Availability

Academic Institutions and Nonprofits only
FRB_nSbMVp

Plasmid

#118969

Purpose

Expresses N-terminus of soybean mosaic virus protease (SbMVp) fused to FKBP-rapamycin binding (FRB) domain

Depositor

Roman Jerala

Publication

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split nSbMVp in fusion with FRB

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
sZF3

Plasmid

#43834

Depositor

George Church

Publication

Mali et al Nat Methods. 2013 Mar 17. doi: 10.1038/n

Insert

sZF3

Tags

HA Flag and Myc

Expression

Mammalian

Promoter

CMV

Available since

March 11, 2013

Availability

Academic Institutions and Nonprofits only
pUC19-T7-Top-T

Plasmid

#87309

Purpose

Expresses the "Top" transcript of the Split-Broccoli system under the control of the T7 promoter.

Depositor

Donald Burke

Publication

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system "Top" transcript

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
(344) pcDNA3.1-HA-nGFP-(EAAAK)4-OGA(544-706)

Plasmid

#168097

Purpose

HA- tagged GFP nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA.

Depositor

Christina Woo

Publication

Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

Insert

HA-nGFP-(EAAAK)4-OGA(544-706)

Tags

HA-Tag

Expression

Mammalian

Promoter

T7/CMV

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-UBC-hAAT-IRES-ZsGreen-W

Plasmid

#126702

Purpose

lentiviral bicistronic exprression of both human alpha-1 anitrypsin and ZsGreen

Depositor

Darrell Kotton

Publication

Wilson et al Am J Respir Cell Mol Biol. 2008 Aug . 39

Insert

Alpha-1 antitrypsin (hAAT aka SERPINA1) and ZsGreen; "AAT-IRES-ZSGreen" (SERPINA1 Human)

Use

Lentiviral

Promoter

UBC

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CMV-BD10-SAS620-3’dCas9-VPR-synpA

Plasmid

#216323

Purpose

Transactivation of murine Myo7b gene via split dCas9-VPR (REVeRT system).

Depositor

Elvir Becirovic

Publication

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Cas9-VPR + splice acceptor site

Use

AAV

Promoter

CMV

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pDONR221_SLC23A3

Plasmid

#132037

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC23A3 (SLC23A3 Human)

Expression

Mammalian

Available since

Nov. 1, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

Enlarge