We narrowed to 51,396 results for: des.1

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Kif5A-CIBN

Plasmid

#102251

Purpose

Expresses fusion of kinesin heavy chain 5A (1-572) and CIB1 (1-170)

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

Kif5A-CIB1 (Kif5a, CIB1 Mustard Weed, Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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Opto-GPR39

Plasmid

#106021

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR39; Opto-GPR39; B11)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR39 (GPR39 Human, Bovine)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-BirA*-KRASG12V

Plasmid

#122208

Purpose

Expresses myc-BirA* fused to KRASG12V in mammalian cells

Depositor

Christopher Counter

Article

Adhikari et al Nat Commun. 2018 Sep 7;9(1):3646. doi: 1

Insert

KRASG12V (KRAS Human)

Tags

myc-BirA*

Expression

Mammalian

Mutation

changed G12 to V

Promoter

CMV

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delBRCT

Plasmid

#173938

Purpose

Bacterial expression of PARP1 lacking BRCT domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delBRCT (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 374-520; V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pSUSL001T:pTRE3G-dCas9-2xKRAB-p2a-tdTomato

Plasmid

#209298

Purpose

A lentiviral vector with tetracycline-inducible system to control expression of S. aureus dCas9 (with tdTomato) to silencing specific genes due to interference with gene transcription machinery.

Depositor

Birgitt Schuele

Article

Sastre et al Sci Rep. 2023 Oct 18;13(1):17796. doi: 1

Inserts

dCas9
tdTomato

Use

Lentiviral

Expression

Mammalian

Promoter

tetracycline-inducible promoter (TRE3G)

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PTK7DN-VSV

Plasmid

#65251

Purpose

Expression of VSV tagged dominant negative PTK7 in mammalian cells

Depositor

Axel Ullrich

Article

Gartner et al PLoS One. 2014 Jan 7;9(1):e84472. doi: 1

Insert

PTK7DN (PTK7 Human)

Tags

VSV

Expression

Mammalian

Mutation

deleted AA 737-1071

Promoter

CMV

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only
pCAGGS 3xFLAG Mbnl2

Plasmid

#217032

Purpose

Overexpression of Mbnl2 in mammalian cells

Depositor

Chaolin Zhang

Article

Weyn-Vanhentenryck et al Nat Commun. 2018 Jun 6;9(1):2189. doi: 1

Insert

Mbnl2 (Mbnl2 Mouse)

Expression

Mammalian

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCW-Cdt1-mCherry-Puro

Plasmid

#193763

Purpose

Doxycyline-inducible (TetOn) human Cdt1 (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

Cdt1-mCherry (CDT1 Human)

Use

Lentiviral

Tags

WT

Mutation

human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

Promoter

TRE

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-hPhyB-mCherry-HRasCT

Plasmid

#100281

Purpose

Expresses Phytochrome B (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells

Depositor

Kazuhiro Aoki

Article

Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

Insert

Phytochrome B (PHYB Mustard Weed)

Tags

mCherry fused with HRas C-terminus for plasma mem…

Expression

Mammalian

Mutation

Human codon optimized PhyB (1-908 aa)

Promoter

CAG promoter

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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PB-PE

Plasmid

#162796

Purpose

All-in-one prime editor piggyBac transposon

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9_ H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

SV40 NLS

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCW-Cdt1ΔPIP-mCherry-Puro

Plasmid

#193765

Purpose

Doxycyline-inducible (TetOn) human Cdt1ΔPIP (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

Cdt1ΔPIP-mCherry (CDT1 Human)

Use

Lentiviral

Tags

mCherry

Mutation

PIP degron removed (a.a.1-19 removed)

Promoter

TRE

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDONOR223 RpH-LAMP1-3xFLAG

Plasmid

#163019

Purpose

gateway donor vector encoding ratiometric sensor of lysosomal lumenal pH

Depositor

Massimiliano Stagi

Article

Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

Insert

RpH-LAMP1-3xFLAG (Lamp1 Mouse)

Use

Gateway system

Tags

3XFLAG

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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MiniCoopR mitfa:BRAF

Plasmid

#118846

Purpose

Expresses human BRAF V600E mutant and zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

BRAF V600E (BRAF Human, Zebrafish)

Use

Zebrafish expression, tol2

Mutation

BRAF V600E

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delZn2

Plasmid

#173935

Purpose

Bacterial expression of PARP1 lacking Zn2 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn2 (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 97-213 (V762A)

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-ART

Plasmid

#174810

Purpose

Bacterial expression of isolated PARP1 ART subdomain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-ART (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-BRCT

Plasmid

#174807

Purpose

Bacterial expression of isolated PARP1 BRCT domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-BRCT (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-WGR

Plasmid

#174808

Purpose

Bacterial expression of isolated PARP1 WGR domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-WGR (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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lenti-EGFR-L858R-T790M-dual-epegRNA

Plasmid

#214100

Purpose

Lentiviral vector expressing epegRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two epegRNAs using independent U6 promoters.

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

EGFR L858R epegRNA/EGFR T790M epegRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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SGyA

Plasmid

#160292

Purpose

Drives expression of Cas9 and a TdTomato fluorescent marker

Depositor

Omar Akbari

Article

Gamez et al Nat Commun. 2021 Dec 10;12(1):7202. doi:

Inserts

Vas promoter
hSpCas9
tdTomato
CTCF insulator
Gypsy insulator
Left homology arm Y chromosome
Right homology arm Y chromosome

Tags

T2A GFP

Expression

Insect

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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R2pH-LAMPx3ALFA

Plasmid

#225136

Purpose

R2pH-LAMP1-labelled lysosomes with 3xLFA

Depositor

Massimiliano Stagi

Article

Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

Insert

Lamp1 (Lamp1 Mouse)

Expression

Mammalian

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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