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pMSCV-miR30-shJAG1 #4

Plasmid

#171197

Purpose

Retroviral vector for U6 promoter driven expression empty miR30 based shJAG1 #4 (to be used in conjunction with Phoenix packaging cells).

Depositor

Masashi Narita

Article

Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do

Insert

shJAG1 #4

Use

Retroviral

Expression

Mammalian

Promoter

U6

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-mGFAP(ABC1D)-ECS-SpyCatcher003

Plasmid

#234545

Purpose

to express SpyCatcher on the extracellular side of astrocytic plasmamembrane

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

ECS-SC

Use

AAV

Mutation

None

Promoter

mGFAP(ABC1D)

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-IgKL-SpyCatcher003-GINKO2

Plasmid

#234544

Purpose

to express a potassium sensor tagged with SpyCatcher in the liver

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

IgKL-SpyCatcher003-GINKO2

Use

AAV

Mutation

None

Promoter

P3

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-mGFAP(ABC1D)-ECS-dSpyCatcher003

Plasmid

#234546

Purpose

to express dSpyCatcher on the extracellular side of astrocytic plasmamembrane

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

ECS-dSC

Use

AAV

Mutation

None

Promoter

mGFAP(ABC1D)

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-IgKL-SpyCatcher003-SEP

Plasmid

#234541

Purpose

to express a pH sensor tagged with SpyCatcher in the liver

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

IgKL-SpyCatcher003-SEP

Use

AAV

Mutation

None

Promoter

P3

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS∆tum

Plasmid

#225198

Purpose

Used to create a markerless deletion of the E. coli HS tum gene

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

tum homology arms

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS_lexA(Ind-)

Plasmid

#225199

Purpose

Used to create a non-inducible lexA by introducing a G85D mutation in E. coli HS

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

lexA homology arms

Expression

Bacterial

Mutation

lexA G85D

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS_gpN:SpyTag

Plasmid

#225193

Purpose

Used for adding C-terminal SpyTag to the E. coli HS P2 phage major capsid protein

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SpyTag

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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GB4635

Plasmid

#215263

Purpose

Module for the expression of HISPS under the copper inducible CBS:mDFR promoter and H3H under the red light regulated Etr8:CMVmin promoter and the constitutive expression of LUZ, CPH,EGFP and p19

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CBS4x:mDFR:HISPS-Etr4:mDFR:H3HSF-LUZ-CPH-EGFP-p19

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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p25.pA.NGFR.mCMV.hPGK.Rab35(WT).wpre[sequenced]

Plasmid

#226764

Purpose

rab35 (WT)

Depositor

Ferdinando Pucci

Article

Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

Insert

rab35 (RAB35 )

Expression

Mammalian

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-GPHN-FingR-mGold-CCR5TC

Plasmid

#231784

Purpose

Visualization of inhibitory post-synapse using GPHN-FingR-mGold

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

GPHN-FingR-mGold-CCR5TC

Expression

Mammalian

Promoter

Actin promoter with CMV enhancer

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-GPHN-FingR-mVenus-CCR5TC

Plasmid

#231783

Purpose

Visualization of inhibitory post-synapse using GPHN-FingR-mVenus

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

GPHN-FingR-mVenus-CCR5TC

Expression

Mammalian

Promoter

Actin promoter with CMV enhancer

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-GPHN-FingR-mGold2t-CCR5TC

Plasmid

#231786

Purpose

Visualization of inhibitory post-synapse using GPHN-FingR-mGold2t

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

GPHN-FingR-mGold2t-CCR5TC

Expression

Mammalian

Promoter

Actin promoter with CMV enhancer

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2t-Rab4a-C-7

Plasmid

#231781

Purpose

Visualization of endosome in mammalian cells using mGold2t

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t-Rab4a-C-7 (RAB4A Human)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2s-Rab4a-C-7

Plasmid

#231773

Purpose

Visualization of endosome in mammalian cells using mGold2s

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s-Rab4a-C-7 (RAB4A Human)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(7CNW)RS

Plasmid

#222874

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Cyano-L-Tryptophan (7-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pM-mKate2

Plasmid

#228629

Purpose

Mammalian expression of mKate2 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mKate2

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pM-LSSmOrange

Plasmid

#228619

Purpose

Mammalian expression of LSSmOrange and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

LSSmOrange

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S724A

Plasmid

#224735

Purpose

For expression of HA-tagged GTSE1 protein with S724A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 724 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S734A

Plasmid

#224736

Purpose

For expression of HA-tagged GTSE1 protein with S734A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 734 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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