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pDisplay sHRPa-KDEL

Plasmid

#73149

Purpose

Large fragment of split HRP targeted for expression in the lumen of the endoplasmic reticulum (ER). CMV promoter.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPa-KDEL

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pDisplay sHRPb-KDEL

Plasmid

#73150

Purpose

Small fragment of split HRP targeted for expression in the lumen of the endoplasmic reticulum (ER). CMV promoter.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPb-KDEL

Tags

V5 tag

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pSpyC-GFP

Plasmid

#220191

Purpose

sgRNA expression vector - pBG42 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG42 promoter with GFP in sgRNA site

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG42

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-SlugCas9-P2A-puro

Plasmid

#214353

Purpose

Expresses SlugCas9 and puromycin resistance genes

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SlugCas9 and puromycin resistance genes

Expression

Mammalian

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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SaCas9_sgRNA_expression_in_pBluescript

Plasmid

#122090

Purpose

U6 driven SaCas9 sgRNA expression vector for cloning own guides

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SaCas9 tracr (NEWENTRY S. pyogenes, Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-mVenus

Plasmid

#154899

Purpose

Expresses a fluorescent protein, mVenus

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Insert

mVenus

Expression

Mammalian

Promoter

EF-1α

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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HA-sav

Plasmid

#32834

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

Sav (SAV1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pMBP-SpyCatcher

Plasmid

#79053

Purpose

Expresses maltose binding protein (MBP) SpyCatcher fusion

Depositor

James Bowie

Article

Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi

Insert

MBP-SpyCatcher

Tags

6xHis and Maltose binding protein

Expression

Bacterial

Mutation

Derived from pDEST14-SpyCatcher (Plasmid #35044)…

Promoter

T7

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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SB52

Plasmid

#21487

Depositor

Shoumo Bhattacharya

Article

Bhattacharya et al Genes Dev. 1999 Jan 1. 13(1):64-75.

Insert

Cited2 (CITED2 Human)

Expression

Mammalian

Available Since

July 23, 2009

Availability

Academic Institutions and Nonprofits only
Lenti-DAG1

Plasmid

#205149

Purpose

Overexpress DAG1

Depositor

Monkol Lek

Insert

Dystroglycan 1 (DAG1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Carries common missense variant c.41C>G (p.Ser…

Promoter

EF-1a

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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E1b-GFP-Tol2-Gateway

Plasmid

#37846

Depositor

Nadav Ahituv

Article

Birnbaum et al Genome Res. 2012 Jun;22(6):1059-68. Epub

Type

Empty backbone

Use

Zebrafish enhancer assay vector

Promoter

E1b

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd8

Plasmid

#42260

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 8 (Fzd8 Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pSV Sport PPAR gamma 1

Plasmid

#8886

Depositor

Bruce Spiegelman

Article

Tontonoz et al Genes Dev. 1994 May 15. 8(10):1224-34.

Insert

PPAR gamma (Pparg Mouse)

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pJMP8923

Plasmid

#220904

Purpose

MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PG-dCas9-novo

Depositor

Jason Peters

Article

Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

Insert

dCas9-novo

Use

CRISPR

Tags

3xMyc

Expression

Bacterial

Mutation

D10A, H840A

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pFYF1327 EGFP Site#2

Plasmid

#47512

Purpose

human gRNA expression vector targeting EGFP

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-EGFP site 2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd3

Plasmid

#42255

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 3 (Fzd3 Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pME-Gal80ts

Plasmid

#51619

Purpose

vertebrate expression of full-length temperature-sensitive Gal80 with stop

Depositor

Joshua Bonkowsky

Article

Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

Insert

Gal80ts (GAL80 Budding Yeast)

Use

Vertebrate expression

Mutation

temperature sensitive (G203V, G323R)*

Available Since

March 27, 2014

Availability

Academic Institutions and Nonprofits only

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pCDH1-hPRMT7-GFP

Plasmid

#87103

Purpose

express hPRMT7 in mammalian cells

Depositor

Min Gyu Lee

Article

Lee et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

Protein arginine methyltransferase 7 (PRMT7 Human)

Expression

Mammalian

Promoter

EF1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pMC_mClover3_BSDminus_F2

Plasmid

#184167

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mClover3
bsd

Use

CRISPR

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pMC_mClover3_BSDminus_F1

Plasmid

#184166

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mClover3
bsd

Use

CRISPR

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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