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pRK5_mEGFP-CRTC1-MAML2-KS

Plasmid

#237672

Purpose

For overexpression of mEGFP-CRTC1-MAML2-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

CRTC1-MAML2-KS (CRTC1, MAML2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLJC2-Blast-DHFR-3xFLAG

Plasmid

#233892

Purpose

lentiviral overexpression vector for DHFR

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

DHFR (DHFR Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAG416-GAL-hnRNPA2B1

Plasmid

#181714

Purpose

Galactose inducible expression of hnRNPA2B1

Depositor

Alejandro Chavez

Insert

hnRNPA2B1 (HNRNPA2B1 Human)

Expression

Yeast

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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shRNA_circHUWE1_2

Plasmid

#215235

Purpose

Supression of shcircHUWE1(19,20)_2 expression

Depositor

Sefi Rosenbluh

Article

Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

Insert

circHUWE1 shRNA 2 (HUWE1 Human)

Use

Lentiviral

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-intron-DjCas13d-pA/EF1a-mCherry-WPRE-pa

Plasmid

#233033

Purpose

To Express HA tagged DjCas13d the CMV promoter. Also encodes a mCherry gene outside of the viral genome

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d and mCherry

Use

AAV

Tags

mCherry

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-intron-hfCas13d-pA/EF1a-mCherry-WPRE-pa

Plasmid

#233034

Purpose

To Express HA tagged hfCas13d the CMV promoter. Also encodes a mCherry gene outside of the viral genome

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

hfCas13d and mCherry

Use

AAV

Tags

mCherry

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pPBbleo-EGF-ScNeo

Plasmid

#209902

Purpose

To monitor the status of EGF, the plasmid encodes a recombinant EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

EGF-ScNeo (EGF )

Tags

GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pPICZ A-SLC25A46[I349D]-His (SB256)

Plasmid

#227605

Purpose

Inducible expression of His-tagged SLC25A46 with I349D mutation in Pichia pastoris

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

10xHis

Expression

Yeast

Mutation

I349D

Promoter

AOX1

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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dCas9 fused to scFv (anti-spike)

Plasmid

#186421

Purpose

Expression of dCas9 with C-terminal nanobody fusion recognizing spike protein from SARS-CoV-2

Depositor

Stephen Elledge

Article

Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

Insert

dCas9-scFv fusion (anti-SARS-CoV-2 spike)

Use

CRISPR

Tags

6His

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19_GNB1L_dTAG

Plasmid

#224378

Purpose

Generates GNB1L dTAG C terminal knock-in cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Tags

dTAG

Expression

Bacterial

Mutation

Synonymous mutation

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_Puro_SFB_GNB1L

Plasmid

#224381

Purpose

Lenti plasmid for generating GNB1L expressing stable cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Expression

Bacterial and Mammalian

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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Pum NaVB2

Plasmid

#200231

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Pitohui uropygialis meridionalis NaV beta 2

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-ALFA-CD28

Plasmid

#223630

Purpose

Lentiviral expression of human CD28 with ALFA tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

ALFA tag

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-Spy-CD28

Plasmid

#223622

Purpose

Lentiviral expression of human CD28 with SpyTag003 in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

Spytag003

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Pert Light chain

Plasmid

#216310

Purpose

Expresses anti-HER2 Pert Light chain in mammalian cells. To be paired with Pert heavy chain to form the anti-HER2 Pert Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

Pert Light chain

Use

Affinity Reagent/ Antibody

Tags

Signal peptide

Expression

Mammalian

Promoter

T7 promoter

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-Alpha-synuclein-A53T

Plasmid

#181722

Purpose

Galactose inducible expression of Alpha-synuclein-A53T

Depositor

Alejandro Chavez

Insert

Alpha-synuclein (SNCA Human)

Expression

Yeast

Mutation

A53T

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pENN491_TALEcharm_MmT8x

Plasmid

#220836

Purpose

TALEcharm Kv2 expression in mammalian cells; targets Prnp

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

TALEcharm

Use

Lentiviral and TALEN

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

EF1a

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pENN456_TALEcharm_Kv2_MmT8x

Plasmid

#220835

Purpose

TALEcharm expression in mammalian cells

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

TALEcharm Kv2

Use

Lentiviral and TALEN

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

EF1a

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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Src(WT)-SsrA(Y7I)-mCer3

Plasmid

#220998

Purpose

This is microTag (Y7I mutation in SsrA)-Src with mCerulean3 at C-terminal

Depositor

Klaus Hahn

Article

Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

Insert

Src, SsrA, mCerulean3 (Src Mouse)

Tags

mCerulean3

Expression

Mammalian

Mutation

Y7I in SsrA

Promoter

CMV

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5_NLS-Halo-eGFP-BioID

Plasmid

#218448

Purpose

expresses the NLS-Halo-eGFP-BioID fusion protein via stable Flp-In integration

Depositor

Thorsten Stafforst

Article

Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

Insert

NLS-Halo-eGFP-BirA

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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