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pDD0257 pCS2-sfCherry11Mx4-10aa-zH2B-SV40NLS-sfGFP-NPLnls

Plasmid

#251772

Purpose

Nuclear reporter for fluorescent protein reconstitution assay

Depositor

Dave Dingal

Article

Lim Suan et al Nat Commun 17, 3257 (2026).

Insert

H2B (hist2h2l Zebrafish)

Tags

4x sfCherry3C small fragments, NLS (SV40) and NLS…

Expression

Mammalian

Promoter

CMV IE94

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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CRISPRi sgSOS1-2

Plasmid

#253093

Purpose

CRISPRi single-guide against SOS1

Depositor

Harish Vasudevan

Article

Vasudevan et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

SOS1 (SOS1 Human)

Use

CRISPR

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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CRISPRi sgNF1-1

Plasmid

#253096

Purpose

CRISPRi single-guide against NF1

Depositor

Harish Vasudevan

Article

Vasudevan et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

NF1 (NF1 Human)

Use

CRISPR

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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CRISPRi sgSOS1-1

Plasmid

#253092

Purpose

CRISPRi single-guide against SOS1

Depositor

Harish Vasudevan

Article

Vasudevan et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

SOS1 (SOS1 Human)

Use

CRISPR

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pUC-FLAG-V2-REPAIR-mini

Plasmid

#252298

Purpose

Expresses REPAIR-mini (V2) in mammalian cells.

Depositor

Hiroshi Nishimasu

Article

Ishikawa et al Nat Struct Mol Biol. 2025 Aug;32(8):1567

Insert

REPAIR-mini (V2)

Expression

Mammalian

Mutation

dPspCas13b H133A/H1058A/Δ929–1090; 30-aa linker; …

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)_His10x-TEV-Rho-tag

Plasmid

#249665

Purpose

Expression of rhodamine-binding protein tag in bacterial cells

Depositor

Kai Johnsson, Richard Wombacher

Article

Kompa et al J Am Chem Soc. 2025 Dec 30. doi: 10.1021

Insert

Rho-tag

Expression

Bacterial

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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TOMO70_NTD_Delta6_GFP

Plasmid

#253341

Purpose

mitochondrial TOMO70 NTD tagged with Delta6 and GFP

Depositor

Qiangjun Zhou

Article

Luo et al bioRxiv [Preprint]. 2026 Jan 20:2026.01.

Insert

TOMO70-NTD-Delta6-GFP (TOMM70 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

UBC

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pFastBac-V2-REPAIR-mini

Plasmid

#252297

Purpose

Expresses REPAIR-mini (V2) in insect cells.

Depositor

Hiroshi Nishimasu

Article

Ishikawa et al Nat Struct Mol Biol. 2025 Aug;32(8):1567

Insert

REPAIR-mini (V2)

Expression

Insect

Mutation

dPspCas13b H133A/H1058A/Δ929–1090; 30-aa linker; …

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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4932 TRV2-TnpB2xSV40nls-HHreRNA4PDS

Plasmid

#251821

Purpose

Editing Photene desaturase (PDS) genes in Nicotiana benthamiana

Depositor

Savithramma Dinesh-Kumar

Article

Nagalakshmi et al Nat Plants. 2026 Mar;12(3):503-511. doi:

Insert

ISDra2 TnpB with guide targeting NbPDS flanked by HH-HDV ribozymes

Tags

2xSV40nls

Expression

Plant

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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4927 TRV2-TnpB-reRNA4PDS

Plasmid

#251814

Purpose

Editing Photene desaturase (PDS) genes in Nicotiana benthamiana

Depositor

Savithramma Dinesh-Kumar

Article

Nagalakshmi et al Nat Plants. 2026 Mar;12(3):503-511. doi:

Insert

ISDra2 TnpB with guide targeting NbPDS with HDV at 3' end

Tags

AtFDnls

Expression

Plant

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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4931 TRV2-TnpB2xSV40nls-reRNA4PDS

Plasmid

#251820

Purpose

Editing Photene desaturase (PDS) genes in Nicotiana benthamiana

Depositor

Savithramma Dinesh-Kumar

Article

Nagalakshmi et al Nat Plants. 2026 Mar;12(3):503-511. doi:

Insert

ISDra2 TnpB with guide targeting NbPDS with HDV at 3' end

Tags

2xSV40nls

Expression

Plant

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)_His10x-TEV-SiR-tag

Plasmid

#249666

Purpose

Expression of silicon rhodamine-binding protein tag in bacterial cells

Depositor

Kai Johnsson, Richard Wombacher

Article

Kompa et al J Am Chem Soc. 2025 Dec 30. doi: 10.1021

Insert

SiR-tag

Expression

Bacterial

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_CD86-Rho-tag

Plasmid

#249669

Purpose

Expression of monomeric rhodamine-binding protein tag on the cell surface in mammalian cells

Depositor

Kai Johnsson, Richard Wombacher

Article

Kompa et al J Am Chem Soc. 2025 Dec 30. doi: 10.1021

Insert

CD86-Rho-tag

Expression

Mammalian

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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UbSYHRE_pDONR201

Plasmid

#249817

Purpose

Encodes UbSYHRE (UBQ-HRE1-GAL4STE12), Gateway compatible as Donor plasmid

Depositor

Francesco Licausi

Article

Lavilla-Puerta et al bioRxiv 2025.09.01.673502

Insert

UBQ-HRE-GAL4STE (ERF71 Budding Yeast, Mustard Weed)

Use

Unspecified

Tags

GAL4-STE12 and UBQ

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pPD684 EnAsCas12a-BFP

Plasmid

#249157

Purpose

Overexpression of EnAsCas12a-BFP with blasticidin resistance gene. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

EnAsCas12a-TagBFP

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pPD686 A2UCOE-EnAsCas12a-BFP

Plasmid

#249159

Purpose

Overexpression of EnAsCas12a-BFP with blasticidin resistance gene. Contains minimal A2UCOE and Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

A2UCOE-EnAsCas12a-TagBFP

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1/Integrin-b5-FL-SGx3-Halo9

Plasmid

#249166

Purpose

Mammalian expression vector of human ITGB5 fused with Halo9-tag

Depositor

Pakorn Kanchanawong

Article

Nishimura et al J Cell Sci. 2025 Nov 24:jcs.264110. doi:

Insert

Integrin beta-5 (ITGB5 Human)

Tags

Halo9

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pHR Myc-Oskar-FusionRed-FRB-iLID

Plasmid

#223289

Purpose

The Oskar germ granule protein tagged with optogenetic and chemogenetic tags for recruitment of desired cargo. Used to demonstrate condensate dissolution using the OptoMBP system.

Depositor

Jared Toettcher

Article

Brumbaugh-Reed et al Nat Commun. 2024 Aug 7;15(1):6717. doi:

Insert

myc-Oskar-FusionRed-FRB-iLID

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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p-att-ef1a-MS2-RecT-dCas9-BSD

Plasmid

#226108

Purpose

Using ef-1a promoter, expresses dCas9 and RecT protein that intended to be tethered via MS2 gRNA hairpin to dCas9, and Blasticidin selection

Depositor

George Church

Article

Oliynyk et al Commun Biol. 2025 Dec 16;8(1):1778. doi:

Insert

BSD

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-GFP

Plasmid

#243759

Purpose

AAV plasmid expressing GFP under the CAG promoter.

Depositor

Andrew Arrant

Article

Nader et al Sci Rep. 2025 Jul 19;15(1):26217. doi: 1

Insert

Enhanced Green Fluorescent Protein

Use

AAV

Promoter

CAG

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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