We narrowed to 21,719 results for: ESC;

1. P789_pY026_EnAsCpf1_CLYBL_T1_catRNA

   Plasmid

   #202760

   Purpose

   Encodes for CRISPR-Cas12a (EnAsCpf1) with gRNA for targeting the CLYBL safe harbor site

   Depositor

   Karl Wahlin

   Article

   Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

   Insert

   huAsCpf1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CaMKIIa-HA-eLACCO2.1-NGR

   Plasmid

   #207989

   Purpose

   Biosensor for extracellular L-lactate

   Depositor

   Yuki Kambe, Robert Campbell

   Article

   Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

   Insert

   eLACCO2.1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CaMKII

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG876 pDisplay-pFAST

   Plasmid

   #172868

   Purpose

   expresses pFAST at the surface of mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

   Insert

   pFAST-PDGFR
   

   Use

   Tags

   IgK leader sequence (N terminal on insert) and My…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PiggyBac EF1a-Tet-on 3G-Puro U6 BbsI

   Plasmid

   #159756

   Purpose

   PiggybacTransposon-based expression control of Tet-on 3G, Puro and Cas13d U6 gRNA backbone

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biomaterials. 2022 May 6;285:121569. doi

   Type

   Empty backbone

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-NES-NIR-GECO1

   Plasmid

   #113683

   Purpose

   Express NIR-GECO1 preferentially in neurons

   Depositor

   Robert Campbell

   Article

   Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

   Insert

   NES-NIR-GECO1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA 3.1(-) mouse Egr2

   Plasmid

   #107997

   Purpose

   Expresses mouse Egr2 in mammalian cells

   Depositor

   Peter Johnson

   Article

   Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

   Insert

   Egr2 (Egr2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Gluc-LHCGR

   Plasmid

   #221781

   Purpose

   Measurement of glycoprotein-glycoprotein receptor binding using the BRET phenomenon and measurement of membrane expression of the Gluc-LHCGR

   Depositor

   Adolfo Rivero-Muller

   Article

   Adamczuk et al Endocrinology. 2024 Apr 29;165(6):bqae05

   Insert

   LHCGR (LHCGR Human)
   

   Use

   Tags

   Gaussia Luciferase and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. LENTICRISPR-TKT_sgRNA2

   Plasmid

   #201625

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

   Insert

   TKT (TKT Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. LENTICRISPR-TKT_sgRNA1

   Plasmid

   #201624

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

   Insert

   TKT (TKT Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTwist-SARS-CoV-2 Δ18 P.1

    Plasmid

    #173476

    Purpose

    Encodes SARS-CoV-2 P.1 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 Spike Truncated to remove 18 amino acids
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-gfaABC1D-HA-deLACCO1-NGR

    Plasmid

    #208099

    Purpose

    Control biosensor for extracellular L-lactate

    Depositor

    Marie-Eve Paquet, Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    R-iLACCO1.1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV6-G1-ATP6-Myc-FLAG (puromycin)

    Plasmid

    #86851

    Purpose

    mammalian expression of ATP6 with mitochondrial targeting sequence, Myc, and FLAG tags

    Depositor

    Matthew O'Connor

    Article

    Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

    Insert

    ATP6 (ATP6 Human)
    

    Use

    Tags

    ATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…

    Expression

    Mammalian

    Mutation

    codon corrected

    Promoter

    CMV

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-HA-PQBP1

    Plasmid

    #25804

    Depositor

    Marius Sudol

    Article

    Tapia et al J Biol Chem. 2010 Apr 21. ():.

    Insert

    PQBP1 (PQBP1 Human)
    

    Use

    Tags

    6xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-CaMKIIa-HA-deLACCO1-NGR

    Plasmid

    #207990

    Purpose

    Control biosensor for extracellular L-lactate

    Depositor

    Yuki Kambe, Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    deLACCO1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKII

    Available since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CaMKIIa-DIO ChRmine-oScarlet-KV 2.1-WPRE

    Plasmid

    #183536

    Purpose

    Optogenetics

    Depositor

    Karl Deisseroth

    Article

    Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

    Insert

    ChRmine-oScarlet-Kv2.1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    CaMKIIa

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. TOP2A sgRNA1

    Plasmid

    #138190

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TOP2A

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    TOP2A (TOP2A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. TOP2A sgRNA2

    Plasmid

    #138191

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TOP2A

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    TOP2A (TOP2A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDSRaD-sdi1

    Plasmid

    #219566

    Purpose

    Mammalian expression of human sdi1 (p21 CDK inhibitor)

    Depositor

    Asao Noda, James R Smith

    Article

    Noda et al Exp Cell Res. 1994 Mar;211(1):90-8. doi:

    Insert

    sdi1 (CDKN1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SRa

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. LSB-hsa-miR-29b-3p

    Plasmid

    #103394

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-29b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-29b-3p target
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. ATG-1929

    Plasmid

    #108712

    Purpose

    CBR2opt in pF4Ag expression vector with T7 and CMV promoter

    Depositor

    Keith Wood

    Article

    Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10

    Insert

    CBR2opt
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promega used directed evolution to create CBR (Cl…

    Promoter

    T7, CMV

    Available since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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