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pTol1-uas-mAKT-2a-nlstdTomato

Plasmid

#67706

Purpose

Tol1 expression plasmid with UAS-driven activated Akt-2A-myc tagged nlsTdTomato

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

mAKT-2A-nlstdTomato

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only
LZF1723: mI12b-EGFP

Plasmid

#104123

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

mI12b

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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AByF

Plasmid

#111084

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Inserts

Y homology arm left
gypsy insulator
3xP3 promoter
tdTomato
SV40 3'UTR
CTCF insulator
Y homology arm right

Expression

Insect

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBACgus_Sumostar/LRRK2 1867-2186_T2031E

Plasmid

#40389

Depositor

Michael J Fox Foundation MJFF

Inserts

SUMO
LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and HisTEV

Mutation

aa1867-2186, T2031E

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pMZ381

Plasmid

#59899

Purpose

Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-L469

Depositor

Mikel Zaratiegui

Article

Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

Inserts

Cas9
rrk1:sgRNA

Use

CRISPR

Expression

Yeast

Mutation

Silent muation of the CspCI site

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pZS1(pLtetO-TP901-aav-proA-tetR)-katGp-PhiC31-proD-oxyR

Plasmid

#78228

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only
pXcX-G4BS-3.6TPH-EGFP

Plasmid

#32572

Depositor

Sergey Kasparov

Article

Benzekhroufa et al BMC Biotechnol. 2009 Mar 19;9:23.

Insert

EGFP

Use

Adenoviral

Expression

Mammalian

Promoter

3.6TPH

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pTLR-donor-UAS

Plasmid

#119001

Purpose

Repair template for the TLR reporter with 10 UAS binding sites for yeast Gal4

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

mVenus

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-M-RAN-RFP6

Plasmid

#106428

Purpose

M-RAN-RFP6 (RANbody RFP6::Sm-MYCtag)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

M-RAN-RFP6

Tags

HA, His, and Multiple MYC

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pERB160

Plasmid

#58285

Purpose

const. Mis12-FKBP3; ind. FRB-mCH-Mad1

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

Mis12-FKBPx3
inducible FRB-mCh-Mad1
FKBP-targeted miRNA

Tags

mCherry

Expression

Mammalian

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pJJH1242

Plasmid

#36909

Depositor

Jürgen Heinisch

Article

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Insert

ScURA3

Use

Lentiviral

Expression

Bacterial, Mammalian, and Y…

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ31f

Plasmid

#39257

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ31 system (no terminator for the transRNA)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1+pLtetO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pERB111

Plasmid

#58281

Purpose

constitutive miRempty_Mis12-GFP-FKBP3; inducible mCh-FRB

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

empty miRNA
Mis12-GFP-FKBPx3
inducible mCh-FRB

Tags

GFP and mCherry

Expression

Mammalian

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pFLLFLN

Plasmid

#40030

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

FRT-Lox5171-Lox2272-FRT-loxP
Neo

Expression

Bacterial and Mammalian

Promoter

None and SV40

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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SK-YFP-ST1-A

Plasmid

#48665

Purpose

Bacterial ST1 repression YFP reporter: protospacer A

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

ST1 prototspacer A/PAM with reporter EYFP

Use

CRISPR

Promoter

lambda pR promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pMST694_BB3_pTet-on_mttA_trpC_pcoxA:pyrGtrunc_AMA2.8

Plasmid

#90292

Purpose

BB3 for mttA integration with homologous flanks at pyrG locus, hygromycin resistance

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

mttA

Use

A niger

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pRAJ11

Plasmid

#39244

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ11 system

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1+pLtetO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ12

Plasmid

#39246

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ12 system

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1+pLtetO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ21

Plasmid

#39249

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ21 system

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1+pLtetO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAWp7-12

Plasmid

#72744

Purpose

pFUGW-UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-124]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-124]

Use

Lentiviral

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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