We narrowed to 11,697 results for: nts

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pTwist-SARS-CoV-2 Spike-deltaC18 BQ.1.1

Plasmid

#212507

Purpose

Encodes SARS-CoV-2 variant BQ.1.1 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike BQ.1.1 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 BA.2.75.2

Plasmid

#212508

Purpose

Encodes SARS-CoV-2 variant BA.2.75.2 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike BA.2.75.2 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 JN.1.18

Plasmid

#223271

Purpose

Encodes SARS-CoV-2 spike JN.1.18 for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike JN.1.18 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 XBB.2.3

Plasmid

#216529

Purpose

Encodes SARS-CoV-2 spike for XBB.2.3 pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike XBB.2.3 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pJT241 Lenti pEF-dCas9-3XFLAG-WT ZNF10 KRAB-T2A-tagBFP-P2A-Blast

Plasmid

#187326

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-WT ZNF10 KRAB-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF1a

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pJT244 Lenti pEF-dCas9-3XFLAG-RYBP-T2A-tagBFP-P2A-Blast

Plasmid

#195537

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dCas9-3XFLAG-RYBP-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF1a

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pJT342 Lenti pEF-dCas9-3XFLAG-RYBP + MGA1+2-T2A-tagBFP-P2A-Blast

Plasmid

#195541

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dCas9-3XFLAG-RYBP + MGA1+2-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF1a

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP8f L27A

Plasmid

#204140

Purpose

Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with L27A mutation

Depositor

Katalin Torok

Article

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f L27A

Tags

N-terminal His tag

Expression

Mammalian

Mutation

Leucine 27 changed to Alanine

Promoter

CMV

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pUC18_BCR_GUSB_e19a2

Plasmid

#190884

Purpose

molecular monitoring of atypical BCR::ABL1 fusion transcripts

Depositor

Nicholas Cross

Article

Schafer et al J Cancer Res Clin Oncol. 2021 Oct;147(10

Inserts

e19a2 BCR::ABL1 fusion cDNA
GUSB cDNA (GUSB Human)
BCR cDNA (BCR Human)

Use

Cloned cdna transcripts for use in rt-qpcr bcr::a…

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pUC18_BCR_GUSB_e8a2

Plasmid

#190883

Purpose

molecular monitoring of atypical BCR::ABL1 fusion transcripts

Depositor

Nicholas Cross

Article

Schafer et al J Cancer Res Clin Oncol. 2021 Oct;147(10

Inserts

e8a2 BCR::ABL1 fusion cDNA
GUSB cDNA (GUSB Human)
BCR cDNA (BCR Human)

Use

Cloned cdna transcripts for use in rt-qpcr bcr::a…

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3-4mut-siRNAres_E

Plasmid

#146193

Purpose

Insect Expression of DmEDC3-4mut-siRNAres

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmEDC3-4mut-siRNAres (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3-5mut-siRNAres_E

Plasmid

#146194

Purpose

Insect Expression of DmEDC3-5mut-siRNA res

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmEDC3-5mut-siRNA res (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-XBB-AVI

Plasmid

#196619

Purpose

Expresses SARS-CoV-2 RBD protein from the XBB variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-XBB

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

G339H, R346T, L368I, S371F, S373P, S375F, T376A, …

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-AVPR2-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194373

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-AVPR2-V2R-NTEV-TCS-GV-2xHA (AVPR2 Synthetic, Human)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmPop2a-D53AE55A-dsRNAres-V5His6_K

Plasmid

#146718

Purpose

Insect Expression of DmPop2a-D53AE55A-dsRNAres

Depositor

Elisa Izaurralde

Insert

DmPop2a-D53AE55A-dsRNAres (Pop2 Fly)

Expression

Insect

Mutation

ORF extracted from CG5684-Pa; NM_140281.2, with o…

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE BI_GFP11-14-SEPT7_SEPT9_i3-14-GFP10

Plasmid

#180349

Purpose

mammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT7 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN9 (SEPTIN9 Human)
IRES-TagBFP

Use

Lentiviral

Tags

GFP10 and GFP11

Expression

Mammalian

Promoter

bidirectional TRE

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pmirGlo-RBM8A-3'UTR 240-546

Plasmid

#158108

Purpose

reporter 3'UTR hRBM8A (short)

Depositor

Emanuela Felley-Bosco

Article

Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

Insert

3'UTR of hRBM8A (RBM8A Human)

Use

Luciferase

Expression

Mammalian

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Spike Display_Part 5 Spacer

Plasmid

#172733

Purpose

Encodes Part 5 of HexaPro-D614G spike to be used in the Spike Display system

Depositor

IIya Finkelstein

Article

Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

Insert

SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)

Use

Synthetic Biology

Mutation

Ectodomain only (AAs 1-1208); 682-685 (furin site…

Available Since

Aug. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Spike Display_Part 4 Spacer

Plasmid

#172732

Purpose

Encodes Part 4 of HexaPro-D614G spike to be used in the Spike Display system

Depositor

IIya Finkelstein

Article

Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

Insert

SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)

Use

Synthetic Biology

Mutation

Ectodomain only (AAs 1-1208); 682-685 (furin site…

Available Since

Aug. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Spike Display_Part 3 Spacer

Plasmid

#172731

Purpose

Encodes Part 3 of HexaPro-D614G spike to be used in the Spike Display system

Depositor

IIya Finkelstein

Article

Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

Insert

SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)

Use

Synthetic Biology

Mutation

Ectodomain only (AAs 1-1208); 682-685 (furin site…

Available Since

Aug. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge