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We narrowed to 11,594 results for: EMB

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Showing: 11521 - 11540 of 11594 results

  1. pTRIP TRE BI_GFP11-14-SEPT7 Gmut1_SEPT9_i1 Gmut-14-GFP10

    Plasmid
    #180355
    Purpose
    mammalian co-expression of human SEPT9_i1 Gmut fused to GFP10 and of human SEPT7 Gmut1 fused to GFP11
    Depositor
    Manos Mavrakis
    Article
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN7 (SEPTIN7 Human)
    SEPTIN 9 (SEPTIN9 )
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    SEPT7 W269A, H279D and SEPT9_i1 W520A, H530D
    Promoter
    bidirectional TRE
    Available Since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180355-plasmid-map-sequence-id-357948

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  2. pTRIP TRE BI_GFP11-14-SEPT7 Gmut1_SEPT9_i3 Gmut-14-GFP10

    Plasmid
    #180356
    Purpose
    mammalian co-expression of human SEPT9_i3 Gmut fused to GFP10 and of human SEPT7 Gmut1 fused to GFP11
    Depositor
    Manos Mavrakis
    Article
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN7 (SEPTIN7 Human)
    SEPTIN 9 (SEPTIN9 )
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    SEPT7 W269A, H279D and SEPT9_i3 W502A, H512D
    Promoter
    bidirectional TRE
    Available Since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180356-plasmid-map-sequence-id-358007

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  3. pTRIP TRE BI_SEPT9_i3 NCmut-14-GFP11_SEPT9_i3 NCmut-14-GFP10

    Plasmid
    #180360
    Purpose
    mammalian co-expression of human SEPT9_i3 NCmut fused to GFP10 and of human SEPT9_i3 NCmut fused to GFP11
    Depositor
    Manos Mavrakis
    Article
    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301
    Inserts
    SEPTIN9_v3 (SEPTIN9 Human)
    SEPTIN9_v3 (SEPTIN9 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    SEPT9_i3 I263D, M270D
    Promoter
    bidirectional TRE
    Available Since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    180360-plasmid-map-sequence-id-357944

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  5. ATX3 13Q- I77K Q78K W87K

    Plasmid
    #185908
    Purpose
    Expresses ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-I77K, Q78K,W87K (N-terminal ) fused with His-Tag and TEV cleavage sequence (C-terminal).
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track and point mutations I77K, Q78K and W87K (ATXN3 Synthetic, Human)
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Point mutations in Atx3 gene I77K Q78K and W87K
    Available Since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    185908-plasmid-map-sequence-id-370341

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  6. pHsSyx3-MBP

    Plasmid
    #179286
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with C-terminal TEV protease cleavage site + mature maltose binding protein + His6
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Tags
    TEV protease cleavage site + mature maltose bindi…
    Expression
    Bacterial
    Mutation
    residues 393-792 from accession number NP_0010361…
    Promoter
    T7
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179286-plasmid-map-sequence-id-354920

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  7. pMBP-HsSyx3

    Plasmid
    #179287
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Tags
    His6 + mature maltose binding protein + TEV prote…
    Expression
    Bacterial
    Mutation
    residues 393-792 from accession number NP_0010361…
    Promoter
    T7
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179287-plasmid-map-sequence-id-354925

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  8. pJ143

    Plasmid
    #29490
    Depositor
    Jonathan Tait
    Article
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Available Since
    June 6, 2011
    Availability
    Academic Institutions and Nonprofits only

  12. pSB-CMV-MCS-Puro ArfGAP1_ALPS1_ALPS2-mKate2-P2A-EGFP-LMNB1

    Plasmid
    #250905
    Purpose
    Dual color expression of mKate2 tagged human ARFGAP1 ALPS 1-2 and EGFP tagged zebrafish LaminB1 in mammallian cell lines
    Depositor
    Philipp Niethammer
    Article
    Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/
    Insert
    ArfGAP1-mKate2-P2A-EGFP-Lamin B1 (lmnb1, ARFGAP1 Human, Zebrafish)
    Tags
    EGFP on LMNB1 and mKate2 on ArfGAP1
    Expression
    Mammalian
    Mutation
    ARFGAP1 amino acids 192–304 only
    Promoter
    CMV
    Available Since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    250905-plasmid-map-sequence-id-498468

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  13. pSB-CMV-MCS-Puro-ArfGAP1_ALPS1-mKate2-3NLS-P2A-eGFP-LMNB1

    Plasmid
    #250914
    Purpose
    Dual color expression of mKate2-3xNLS tagged rat ALPS1 and eGFP tagged zebrafish Lamin B1 in mammalian cell lines
    Depositor
    Philipp Niethammer
    Article
    Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/
    Insert
    ArfGAP1-mKate2-3xNLS-P2A-EGFP-LMNB1 (lmnb1, Arfgap1 Rat, Zebrafish)
    Tags
    EGFP on LMNB1 and mKate2 on ArfGAP1
    Expression
    Mammalian
    Mutation
    ARFGAP1 amino acids 196–234 only
    Promoter
    CMV
    Available Since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    250914-plasmid-map-sequence-id-498467

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  14. pLV-RnSyt1-sgRNA2-dCas9-KRAB-TagBFP2 (identifier AAAA-0246)

    Plasmid
    #202555
    Purpose
    Synaptotagmin-1 sgRNA2 with TagBFP2
    Depositor
    Frederic Meunier
    Article
    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:
    Insert
    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCAGTACTCGCGTGCCTCGCACCGG) (Syt1 Rat)
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202555-plasmid-map-sequence-id-404691

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  15. pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

    Plasmid
    #202556
    Purpose
    Synaptotagmin-1 sgRNA3 with TagBFP2
    Depositor
    Frederic Meunier
    Article
    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:
    Insert
    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202556-plasmid-map-sequence-id-404689

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  16. pLV-RnSyt1-sgRNA1-dCas9-KRAB-TagBFP2 (identifier AAAA-0245)

    Plasmid
    #202554
    Purpose
    Synaptotagmin-1 sgRNA1 with TagBFP2
    Depositor
    Frederic Meunier
    Article
    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:
    Insert
    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ -CACCGCGTGCCTCGCACCGGTCCGCGG) (Syt1 R. norvegius (gRNA))
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202554-plasmid-map-sequence-id-404687

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  17. pTRE3G-mouse cGASdeltaN-GFP

    Plasmid
    #186878
    Purpose
    Doxycycline-dependent expression of mouse cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Mouse cGAS truncation mutant (148-508 a.a.) with C-terminal GFP fusion (Cgas Synthetic, Mouse)
    Use
    Retroviral
    Tags
    GFP
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available Since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186878-plasmid-map-sequence-id-366111

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  18. pMBP-HsSyx-375-792

    Plasmid
    #179291
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Tags
    His6 + mature maltose binding protein + TEV prote…
    Expression
    Bacterial
    Mutation
    residues 375-792 from accession number NP_0010361…
    Promoter
    T7
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179291-plasmid-map-sequence-id-354915

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  19. pcDNA3.1 Flag YFP hNKCC1 P676C I730C (NT809)

    Plasmid
    #50866
    Purpose
    Expresses human NKCC1 P676C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    P676C I730C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50866-plasmid-map-sequence-id-76339

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  20. pcDNA3.1 Flag YFP hNKCC1 I674C (NT475)

    Plasmid
    #50875
    Purpose
    Expresses human NKCC1 I674C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    I674C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50875-plasmid-map-sequence-id-76348

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Showing: 11521 - 11540 of 11594 results