Addgene

We narrowed to 4,334 results for: 187

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Myc-PSFΔPQ

Plasmid

#35187

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Deletion of residues 34-113

Promoter

CMV

Available since

Oct. 2, 2012

Availability

Academic Institutions and Nonprofits only
sgRNA AAVS1

Plasmid

#128119

Purpose

Co-expresses sgRNA AAVS1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA AAVS1 + GFP

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

RSV/U6

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Rca_Cas13a_WT

Plasmid

#91870

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

RcaCas13a (RcaC2c2) WT

Use

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pXS-dTomato

Plasmid

#117387

Purpose

pGEN-mcs with a modular dTomato expression scaffold

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Insert

dTomato

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pNLS-LSSmKate1-N1

Plasmid

#31870

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

NLS

Use

Tags

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Promoter

CMV

Available since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only

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Cas-Acr (insertion 5)

Plasmid

#128116

Purpose

Expresses SpyCas9 fused to attenuated AcrIIA4 (insertion 5)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

CascAID (insertion 5)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Cas-Acr (N39A)

Plasmid

#128117

Purpose

Expresses SpyCas9 fused to attenuated AcrIIA4 (N39A)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

CascAID (N39A)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pNLS-LSSmKate2-N1

Plasmid

#31871

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

NLS

Use

Tags

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

Promoter

Available since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only

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Cas-Acr (wtAcrIIA4)

Plasmid

#128115

Purpose

Expresses SpyCas9 fused to wild-type AcrIIA4

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

CascAID (wtAcrIIA4)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA EMX1

Plasmid

#128121

Purpose

Co-expresses sgRNA EMX1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA EMX1 + GFP

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

RSV/U6

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pSuperRetro mouse Hic-5

Plasmid

#11187

Depositor

Bruce Spiegelman

Article

Drori et al Genes Dev. 2005 Feb 1. 19(3):362-75.

Insert

Hic-5 shRNA (Tgfb1i1 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pFOSGE_CUS1E04

Plasmid

#71076

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Sirt4 (Sirt4 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pc3XB-ZF137

Plasmid

#13187

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF137

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 solvvol W

Plasmid

#231878

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 solvvol W

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 solvvol W

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

T7

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 solvvol

Plasmid

#231877

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 solvvol

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 solvvol

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

T7

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 ILVtoED W

Plasmid

#231871

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED W

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 ILVtoED W

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

N126W, I128E, V130E, V135E, L137D, I142D

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-G-Gal4-VPR

Plasmid

#229187

Purpose

lvl 0 5 gene

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

Gal4-VPR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCF.111

Plasmid

#227187

Purpose

pORTMAGE-Ec1 + supplement E. coli SSB

Depositor

Seth Shipman

Article

Fishman et al Nat Biotechnol. 2024 Sep 5. doi: 10.1038

Insert

CspRecT, mutL_E32K, E. coli SSB

Use

Tags

Expression

Bacterial

Mutation

mutL E32K

Promoter

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-Vamp2-GeneTRAP

Plasmid

#218187

Purpose

To KO and genetrap mouse Vamp2 simultaneously

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Vamp2 Mouse)

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-BBS18

Plasmid

#218718

Purpose

Expresses N-terminally EGFP-tagged BBS18 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

BBS18

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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