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pet23a_RBPMS_20_196

Plasmid

#65734

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 20-196

Promoter

T7

Available since

July 13, 2015

Availability

Academic Institutions and Nonprofits only

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pet23a_RBPMS_1_129

Plasmid

#65731

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-129

Promoter

T7

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pet23a_RBPMS_1_111

Plasmid

#65729

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-111

Promoter

T7

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pet28a_RBPMS_20_196

Plasmid

#65741

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 20-196

Promoter

T7

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-2TK-WW-EF1+EF2

Plasmid

#18080

Depositor

Marius Sudol

Article

Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

Insert

Dystrophin (DMD Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pEC3115 (pSpy0K6-P23_mKate2~*ssrA)

Plasmid

#218509

Purpose

integrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombination

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

mKate2

Use

Tags

ssrA degradation tag

Expression

Bacterial

Mutation

Deletion of Plac_mrfp cassette, introduction of a…

Promoter

P23

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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HDAC4 Flag

Plasmid

#13821

Purpose

Mammalian expression of human histone deacetylase 4 with flag tag

Depositor

Eric Verdin

Article

Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

Insert

HDAC4 (HDAC4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pY003 (pFnCpf1_delta Cas)

Plasmid

#69974

Purpose

Expresses FnCpf1 and spacers 1-4 of CRISPR array. Cas genes are removed.

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 locus without Cas genes

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Cas genes are deleted from locus

Promoter

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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MLF2-GFP-3xFLAG

Plasmid

#230986

Purpose

Expresses GFP tagged MLF2 in mammalian cells

Depositor

Christian Schlieker

Article

Prophet et al Nat Cell Biol. 2022 Nov;24(11):1630-1641

Insert

MLF2 (MLF2 Human)

Use

Tags

3xFLAG and GFP

Expression

Mammalian

Mutation

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1alpha-soCoChR-tdTomato

Plasmid

#107714

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoter

Depositor

Edward Boyden

Article

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Insert

soCoChR-tdTomato

Use

AAV

Tags

KA2 and tdTomato

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pY002 (pFnCpf1_min)

Plasmid

#69975

Purpose

Expresses FnCpf1 and spacers 1-4 of CRISPR array under artificial promoter. Cas genes are removed.

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 locus without Cas genes nd artificial promoter

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/FLAG/HA-DEST DICER

Plasmid

#19881

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Oct 31. ():.

Insert

Dicer 1, ribonuclease type III (DICER1 Human)

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin-FLEX-soCoChR-GFP

Plasmid

#107712

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Cre-dependent virus

Depositor

Edward Boyden

Article

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Has Service

AAV9

Insert

soCoChR-GFP

Use

AAV

Tags

EGFP and KA2

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin- soCoChR-GFP

Plasmid

#107708

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Human Synapsin (hSynapsin) promoter

Depositor

Edward Boyden

Article

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Has Service

AAV9

Insert

soCoChR-GFP

Use

AAV

Tags

EGFP and KA2

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_G3BP1_G1

Plasmid

#127114

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA G3BP1 (G3BP1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_eGFP_CELF1

Plasmid

#106100

Purpose

Inducible gene expression of EGFP - CELF1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

CELF1 (CELF1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pNAD1

Plasmid

#154347

Purpose

pUC19 backbone with editing template for homologous recombination mediated deletion of Nitrate reductase gene of N. oceanica and selection based on zeocin resistance

Depositor

John van der Oost

Article

Naduthodi et al Biotechnol Biofuels. 2019 Mar 25;12:66.

Insert

ShBle

Use

Microalgae

Tags

Expression

Bacterial

Mutation

none

Promoter

Violaxanthin Chlorophyll a Binding Protein Precur…

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsEDC4

Plasmid

#66597

Purpose

Expresses lambdaN-HA-tagged EDC4 in mammalian cells

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

EDC4 (EDC4 Human)

Use

Tags

lambdaN-HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pOP22-5'UTR-BBa_B0034

Plasmid

#216626

Purpose

NanoLuc reporter plasmid to optimize chloroplast cell-free systems with RBS BBa_B0034 in 5'UTR

Depositor

Henrike Niederholtmeyer

Article

Bohm et al ACS Synth Biol. 2024 Aug 16;13(8):2412-2

Insert

T7p-5'B0034-NanoLuc-3'TMV

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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pOP01-UTC-7.0

Plasmid

#216625

Purpose

NanoLuc reporter plasmid to optimize chloroplast cell-free systems, universal test construct

Depositor

Henrike Niederholtmeyer

Article

Bohm et al ACS Synth Biol. 2024 Aug 16;13(8):2412-2

Insert

T7p-5'g10-NanoLuc-3'TMV

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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