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GFP-Myo 1g

Plasmid

#134991

Purpose

Expression of mouse Myo 1g in mammalian cells.

Depositor

Martin Bähler

Article

Olety et al FEBS Lett. 2010 Feb 5;584(3):493-9. doi:

Insert

Myo 1g (Myo1g Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pMB22

Plasmid

#122244

Purpose

Lentivirus delivery for stable expression of AsCas12a-VPR

Depositor

Darjus Tschaharganeh

Article

Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

Insert

mammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator

Use

Lentiviral

Tags

NLS and VPR activator

Expression

Mutation

Promoter

EFs

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pBSFI-p110δ

Plasmid

#34893

Depositor

Peter Vogt

Article

Kang et al Proc Natl Acad Sci U S A. 2006 Jan 31;10

Insert

p110δ (PIK3CD Human)

Use

Adaptor plasmid

Tags

Expression

Mutation

See depositor comments below.

Promoter

Available since

Feb. 23, 2012

Availability

Academic Institutions and Nonprofits only

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FUW-mTurquoise

Plasmid

#193092

Purpose

constitutive expression of mTurquoise in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

mTurquoise

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Ubc

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV Syn intron Psam4GlyR m3m4 HA

Plasmid

#196041

Purpose

AAV for Chemogenetic plasmids

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Insert

Psam4GlyR m3m4 HA

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pICH47732:PtFCP:ShBle

Plasmid

#104893

Purpose

Expresses ShBle with FCP promoter/terminator, Golden Gate Assembly, for Phaeodactylum transformation.

Depositor

Thomas Mock

Article

Graff van Creveld et al Front Microbiol. 2021 Sep 8;12:688199. d

Insert

ShBle

Use

Phaeodactylum; golden gate assembly

Tags

PtFCP promoter and terminator

Expression

Mutation

Promoter

Available since

Dec. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-mIFITM1

Plasmid

#58415

Purpose

Expresses murine IFITM1 with an N-terminal HA tag in mammalian cells.

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only
pTORKm43GWC

Plasmid

#112898

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mTFP1.

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Tags

Expression

Mutation

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTH

Plasmid

#56659

Purpose

Dual-expression vector for P. tricornutum

Depositor

Philip Weyman

Article

Weyman et al Plant Biotechnol J. 2014 Oct 10. doi: 10

Insert

KanR

Use

Synthetic Biology; Diatom expression

Tags

Expression

Mutation

Promoter

Available since

Oct. 14, 2014

Availability

Academic Institutions and Nonprofits only

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FCK-Arch-CFP

Plasmid

#22221

Purpose

Lentiviral expression of Arch-CFP for optogenetic inhibition

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Arch

Use

Lentiviral

Tags

CFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pTORKm43GWY

Plasmid

#112901

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mCitrine.

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Tags

Expression

Mutation

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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MPC020: CamKII CMV_NovArch_citrine

Plasmid

#153193

Purpose

Encodes Novarch

Depositor

Adam Cohen

Article

Chien et al Sci Adv. 2021 May 5;7(19). pii: 7/19/eab

Insert

NovArch

Use

Tags

citrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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RAB14 3'UTR WT

Plasmid

#61489

Purpose

luciferase reporter containing RAB14 3' UTR

Depositor

Curt Civin

Article

Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

Insert

RAB14 3' UTR (RAB14 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

contains position 837-1637 (numbering relative to…

Promoter

PGK

Available since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only
HIPK1-myc-his

Plasmid

#1850

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

HIPK1 (Hipk1 Mouse)

Use

Tags

his and myc

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
pMH-HT_CtCHAD

Plasmid

#127785

Purpose

Expresses CtCHAD in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

CtCHAD (CT0884 Chlorobium tepidum)

Use

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Residues 208-522 (CHAD domain)

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pPT-PCR C2

Plasmid

#141247

Purpose

PCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 2

Depositor

Bogumil Karas

Article

Cochrane et al Algal Research, 2020. 50, 102014

Insert

Mitochondrial Genome of Phaeodactylum tricornutum

Use

Synthetic Biology; Diatom expression

Tags

Expression

Bacterial and Yeast

Mutation

Repeat region removed, additional minor mutations…

Promoter

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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DAXX-myc

Plasmid

#1851

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

DAXX (Daxx Mouse)

Use

Tags

myc

Expression

Bacterial

Mutation

Promoter

Available since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
pMH-HT_SsCHAD

Plasmid

#127783

Purpose

Expresses SsCHAD in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

SsCHAD (SULM_RS11045 Sulfolobus solfataricus)

Use

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCASP-HilA empty vector

Plasmid

#153333

Purpose

Triggers HilA overexpression from Salmonella upon induction with arabinose without any synthetic protein expressed

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Type

Empty backbone

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBM23-pyrF-NT

Plasmid

#174384

Purpose

pBM23 contained pyrF and mini-CRISPR.

Depositor

Jing Han

Article

Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

Insert

pyrF-NT

Use

Template for cloning fragments for chromosomal in…

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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