We narrowed to 24,051 results for: Nes

1. pLSC-5

   Plasmid

   #62889

   Purpose

   Lenti vector for expression of inducible split Cas9

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Inserts

   SpCas9(574-1368)
   SpCas9(2-573)
   

   Use

   CRISPR and Lentiviral

   Tags

   FFBP12, FRB, NES PTK2, NLS SV40, and P2A

   Expression

   Mammalian

   Mutation

   *see comment below

   Promoter

   EFS

   Available since

   March 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX855

   Plasmid

   #62887

   Purpose

   N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa 2-535)
   

   Use

   CRISPR

   Tags

   FRB and NES PTK2

   Expression

   Mammalian

   Mutation

   Aspartic acide 10 to Alanine (D10A)

   Promoter

   CBh

   Available since

   March 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Cyto-GCaMP3

   Plasmid

   #64853

   Purpose

   Cytosol-targeted version of the Ca2+ indicator GCaMP3.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   GCaMP3
   

   Use

   Tags

   Nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmax-3xFLAG_3xNLS_dPspCas13b(AAAA)_2xNLS-Clover

   Plasmid

   #196829

   Purpose

   Express dpdpCas13b with AAAA mutation and Clover

   Depositor

   Albert Cheng

   Article

   Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

   Insert

   dpspCas13b(AAAA)
   

   Use

   Tags

   3XFLAG and NES

   Expression

   Mammalian

   Mutation

   K367A, D369A and K370A

   Promoter

   Available since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTL

   Plasmid

   #203176

   Purpose

   Centromeric yeast expression vector, leucine selection

   Depositor

   William Sessa

   Article

   Galosi et al Brain. 2022 Mar 29;145(1):208-223. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)-Cyto-CaNARi

   Plasmid

   #64849

   Purpose

   Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CaNARi
   

   Use

   Tags

   Cerulean, Nuclear export signal (NES), and Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. MCP-ADAR2-DD

   Plasmid

   #170124

   Purpose

   Lentiviral vector carrying the MCP-ADAR2 with a nuclear export signal

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   MCP-ADAR2-DD-NES
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a core promoter

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQP-NiRISB

   Plasmid

   #138977

   Purpose

   Light-controlled tri-directional targeting of endogenous proteins (nucleus-shifted)

   Depositor

   Vladislav Verkhusha

   Article

   Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

   Insert

   NcoI-BphP1-CAAX-IRES2-NES- iB(GFP)- mCherry-Q-PAS1-AsLOV2cNLS-NotI
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAV0479

   Plasmid

   #133499

   Purpose

   Expression of sfGFP excluded from the nucleus with two NES signals

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pHis5(StuI)-p(tdh1)-NESwis1-sfGFP-NESmia1-natMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Split-RESCUE-C

    Plasmid

    #170154

    Purpose

    AAV vector carrying the RESCUE-DDC-λN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    RESCUE-DDC-λN-NES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQP-iRISB

    Plasmid

    #138979

    Purpose

    Light-controlled tri-directional targeting of endogenous proteins

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    NcoI-BphP1-CAAX-IRES2-NES-SGlinkeriB( GFP)-mCherry-Q-PAS1-AsLOV2cNLS-NotI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTL-DHDDS

    Plasmid

    #203177

    Purpose

    Centromeric yeast expression vector for hDHDDS

    Depositor

    William Sessa

    Article

    Galosi et al Brain. 2022 Mar 29;145(1):208-223. doi:

    Insert

    DHDDS (DHDDS Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    RER2

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. SLF063: Lenti-fCAG-jRGECO1a

    Plasmid

    #124775

    Purpose

    For general lentiviral or direct expression of cytosolic jRGECO1a

    Depositor

    Adam Cohen

    Article

    Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

    Insert

    jRGECO1a
    

    Use

    Lentiviral

    Tags

    NES-6xHIS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

14. FKBP-NTEVp(WT)

    Plasmid

    #137831

    Purpose

    Cytosolic split-TEV protease chain with FKBP Rapamycin sensing domain, N-terminal WT TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FKBP-NTEVp(WT)
    

    Use

    Tags

    3x FLAG and NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. FRB-CTEVp(WT)

    Plasmid

    #137832

    Purpose

    Cytosolic split-TEV protease chain with FRB Rapamycin sensing domain, C-terminal WT TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FRB-CTEVp(WT)
    

    Use

    Tags

    3x FLAG and NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-cytoEKAR4

    Plasmid

    #174438

    Purpose

    Cytosol-targeted EKAR4.

    Depositor

    Jin Zhang

    Article

    Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

    Insert

    cytoEKAR4
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cyto-ABKAR

    Plasmid

    #61510

    Purpose

    Expression of AMPK biosensor

    Depositor

    Jin Zhang, Takanari Inoue

    Article

    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

    Insert

    AMPK Biosensor
    

    Use

    Tags

    NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. MCP-ADAR2-DD(E488Q)

    Plasmid

    #170125

    Purpose

    Lentiviral vector carrying the MCP-ADAR2(E488Q) with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    MCP-ADAR2-DD(E488Q)-NES
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a core promoter

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. LAMP1-APEX2

    Plasmid

    #215555

    Purpose

    Stable, inducible expression in lysosome

    Depositor

    Nevan Krogan

    Article

    Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

    Insert

    LAMP1-EGFP-Flag tag-APEX2-NES
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Cyto-APEX2

    Plasmid

    #215552

    Purpose

    Stable, inducible expression in cytoplasm

    Depositor

    Nevan Krogan

    Article

    Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

    Insert

    EGFP-Flag tag-APEX2-NES
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge