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pET21b - S. thermophilus DltB

Plasmid

#132607

Purpose

expresses S. thermophilus DltB in E. coli

Depositor

Wenqing Xu

Article

Ma et al Nature. 2018 Oct;562(7726):286-290. doi:

Insert

Dltb

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-mAIM2-GST

Plasmid

#51533

Purpose

Express GST-tagged mAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

lac operator

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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pAR69_STRI_m(KS_MAT)_H8_pET22b

Plasmid

#122849

Purpose

Expresses the condensing part of murine type I fatty acid synthase (FASN) consisting of the KS and MAT domain in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

Depositor

Martin Grininger

Article

Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

Insert

m(KS_MAT) (Fasn Mouse)

Use

Tags

His8 tag and Twin-Strep tag

Expression

Bacterial

Mutation

wildtype

Promoter

T7 promoter

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-A762V

Plasmid

#174795

Purpose

Bacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-A762V (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

A762V

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28b-trAtCCD8(pYL736)

Plasmid

#178287

Purpose

Expresses trAtCCD8 in E. coli. pET28b carrying t N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

trAtCCD8

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-PKM2-H391Y

Plasmid

#122692

Purpose

Express His-tagged PKM2 (H391Y) protein in bacteria.

Depositor

Wen-Ching Wang

Article

Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

Insert

Pyruvate kinase M2 isoform (PKM Human)

Use

Tags

6His-tag

Expression

Bacterial

Mutation

H391Y

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a His6Flag2AUF1(p42)

Plasmid

#135941

Purpose

Expresses AUF1p42 in E. coli

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Use

Tags

Flag2 and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyTag002 DA-MBP

Plasmid

#102832

Purpose

Bacterial expression of unreactive version of SpyTag002 fused to maltose binding protein

Depositor

Mark Howarth

Article

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

SpyTag002 DA-MBP

Use

Tags

His6 and Thrombin cleavage site

Expression

Bacterial

Mutation

Reactive aspartic acid has been mutated to an ala…

Promoter

T7

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pET28-At_PRORP1-His

Plasmid

#67869

Purpose

bacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tag

Depositor

Walter Rossmanith

Article

Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

Insert

PRORP1 (PRORP1 Mustard Weed)

Use

Tags

His

Expression

Bacterial

Mutation

Residues 36-572 (see comments)

Promoter

T7

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-2

Plasmid

#172739

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-2

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-PTBP1-RRM12CCtoSS

Plasmid

#89155

Purpose

Recombinant expression of PTBP1-RRM12 C250S,C251S in E.coli

Depositor

Frédéric Allain

Article

Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

Insert

Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S (PTBP1 Human)

Use

Tags

hexa His

Expression

Bacterial

Mutation

mutations: Cys 250 to Ser, Cys 251 to Ser

Promoter

T7

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-ClbN-NHis

Plasmid

#51497

Purpose

NRPS module containing CATE domains with N-terminal 6His tag

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbN

Use

Tags

6x His tag and T7 tag

Expression

Bacterial

Mutation

contains aa30-1094 of NP_754347.1

Promoter

T7

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pET28-p53 (1-73) (72R)

Plasmid

#62082

Purpose

Expression of human p53 (1-73) (72R) in e. coli

Depositor

Gary Daughdrill

Article

Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

Insert

p53 (TP53 Human)

Use

Tags

HIs

Expression

Bacterial

Mutation

contains TAD residues 1-73; 72R

Promoter

T7

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
pET28a-Hook3 aa 1-239

Plasmid

#74609

Purpose

Expresses Hook domain and first coiled coil of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239 (HOOK3 Human)

Use

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pET28b+R3(R112C/T180C)

Plasmid

#108195

Purpose

Expresses PdR-PCNA3(R112C/T180C) fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PdR-PCNA3(R112C/T180C) fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

R112C/T180C(PCNA3)

Promoter

T7 promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET28 mSmo-Cterm

Plasmid

#58703

Purpose

used for anti-mouse Smoothened antibody production (for affinity purification)

Depositor

Matthew Scott

Article

Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

Insert

Smo (Smo Mouse)

Use

Tags

6X-HIS

Expression

Bacterial

Mutation

contains C terminus (aa 543-793)

Promoter

Available since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only
pET29NK-LGK/mGFPmut3

Plasmid

#81168

Purpose

GFP fusion to levoglucosan kinase

Depositor

Timothy Whitehead

Article

Wrenbeck et al ACS Synth Biol. 2019 Mar 15;8(3):474-481

Insert

Levoglucosan kinase wild-type

Use

Tags

His6x and mGFPmut3

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pET28b Me31B-3Myc

Plasmid

#113001

Purpose

For protein expression and purification of Myc-tagged full-length Drosophila me31B

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length me31B (me31B Fly)

Use

Tags

Myc

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-Ava3858-3857

Plasmid

#45744

Depositor

Emily Balskus, Christopher T Walsh

Article

Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

Insert

Ava3858-3857

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only
pET22b-ecDHFR-G51PEKN

Plasmid

#118582

Purpose

Bacterial expression of E.coli DHFR G51PEKN mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Use

Tags

Expression

Bacterial

Mutation

G51 mutated to P51; Glu-Lys-Asn residues inserted…

Promoter

T7

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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