We narrowed to 6,905 results for: crispr cas9 plasmids

1. pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A7-gRNA_(CJT89)

   Plasmid

   #226986

   Purpose

   pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A7)

   Depositor

   Benjamin Kleinstiver

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   SpCas9 gRNA A7 to create OPA1-R194G
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2b_(CJT93)

   Plasmid

   #226992

   Purpose

   pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2b must be used with gRNA 1a or 1b

   Depositor

   Benjamin Kleinstiver

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   SpCas9 gRNA 2b to create OPA1-del_exon5
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1b_(CJT92)

   Plasmid

   #226990

   Purpose

   pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1b must be used with gRNA 2a or 2b

   Depositor

   Benjamin Kleinstiver

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   SpCas9 gRNA 1b to create OPA1-del_exon5
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A9-gRNA_(CJT87)

   Plasmid

   #226988

   Purpose

   pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A9)

   Depositor

   Benjamin Kleinstiver

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   SpCas9 gRNA A9 to create OPA1-R194G
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSaCas9-1xPP7-2x3'UTR

   Plasmid

   #122947

   Purpose

   For expressing SaCas9 mRNA with one copy of PP7 aptamer and 2 copies of HBB 3' UTR

   Depositor

   Baisong Lu

   Article

   Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

   Insert

   SaCas9-PP7-HBB 3' UTR
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSaCas9-1xms2-2x3’UTR

   Plasmid

   #122946

   Purpose

   For expressing SaCas9 mRNA with one copy of MS2 aptamer and 2 copies of HBB 3' UTR

   Depositor

   Baisong Lu

   Article

   Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

   Insert

   SaCas9-MS2-HBB 3' UTR
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pX459-HypaCas9-mR2-ACTB_sgRNA

   Plasmid

   #183884

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ACTB sgRNA for N-terminal tagging of beta-actin in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ACTB sgRNA spacer (ACTB Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX330-U6-E690_guide-CBh-hSpCas9

   Plasmid

   #188545

   Purpose

   Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid E690

   Depositor

   Darrell Kotton

   Article

   Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   T2A-EGFP

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX330-U6-W308_guide-CBh-hSpCas9

   Plasmid

   #188546

   Purpose

   Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid W308

   Depositor

   Darrell Kotton

   Article

   Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   T2A-EGFP

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX330-U6-L101_guide-CBh-hSpCas9

    Plasmid

    #188547

    Purpose

    Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid L101

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    T2A-EGFP

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSEVA47_dCas9-GFP_NOT_i1

    Plasmid

    #231416

    Purpose

    This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-1.

    Depositor

    Manish Kushwaha

    Article

    Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

    Inserts

    dCas9
    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-CMV-FLEX-SaCas9-U6-sgCnr1

    Plasmid

    #209196

    Purpose

    Mutagenesis of Cnr1

    Depositor

    Larry Zweifel

    Article

    Gunduz-Cinar et al Neuron. 2023 Jul 19:S0896-6273(23)00482-

    Insert

    Cnr1 (Cnr1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-CMV-FLEX-SaCas9-U6-sgKcnma1

    Plasmid

    #209199

    Purpose

    Mutagenesis of Kcnma1

    Depositor

    Larry Zweifel

    Article

    Juarez et al Sci Adv. 2023 Aug 11;9(32):eadg8869. doi

    Insert

    Kcnma1 (Kcnma1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSpCas9_T2A_mCherry sg1-sg2

    Plasmid

    #192479

    Purpose

    All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Use

    Tags

    T2A mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Cas9_YTHDF2_sgRNA

    Plasmid

    #186673

    Purpose

    YTHDF2 sgRNA plasmid

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    YTHDF2 KO sgRNA Plasmid (YTHDF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pINDUCER dCas9-TET1CDMut

    Plasmid

    #101920

    Purpose

    The dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.

    Depositor

    Danwei Huangfu

    Article

    Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

    Insert

    dCas9-TET1CD Mut
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Mutated TET1 catalytic domain

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX459_V2.0_pSpCas9(BB)-2A-Puro_T-REX17_Ex1_KI

    Plasmid

    #195504

    Purpose

    Cas9-2A-Puro expression vector bearing a sgRNA targeting Exon 1 of human lncRNA T-REX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    3XFLAG, Puro, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSLQ2818_Blasti_pPB: CAG-PYL1-KRAB-IRES-Blasti-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9-FKBP_F36V

    Plasmid

    #187959

    Purpose

    Expressed ABA-inducible dimerizing KRAB-dCas9 system, with KRAB-IRES-Blasticidin resistance under CAG promoter and tagBFP-dCas9-FKBP12 (F36V mutant) degron under PGK promoter in a piggyBac plasmid.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Inserts

    Blasticidin resistance
    FKBP12_F36V
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Icam 2 SaCas9 YAP sgRNA3

    Plasmid

    #99736

    Purpose

    Expresses SaCas9 and a gRNA targeting mouse YAP

    Depositor

    Yu Huang

    Article

    Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

    Insert

    SaCas9 gRNA targeting Yap1 (Yap1 Mouse)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-CMV-SauriCas9-KKH-Puro

    Plasmid

    #135966

    Purpose

    Expresses SauriCas9-KKH and puromycin resistance genes, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge