We narrowed to 12,753 results for: CAR

1. pBABE-3xFLAG-OB D166R

   Plasmid

   #79061

   Purpose

   Retroviral expression of 3xFLAG tagged, nuclear localized TPP1-OB-RR folding domain

   Depositor

   Steven Artandi

   Article

   Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

   Insert

   TPP1 (TPP1 Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Aspartic acid 166 changed to Arginine

   Promoter

   CMV

   Available since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pBW301ParsR-Amp30T

   Plasmid

   #78653

   Purpose

   pSB3K3 carrying araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-30hrpR-30hrpS-t-hrpL-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

   Insert

   araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   see insert

   Available since

   Aug. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

3. GST-PRMT9 deltaC 350 (stop)

   Plasmid

   #67612

   Purpose

   bacterial expression of GST-PRMT9 missing residues 351-845

   Depositor

   Steven Clarke

   Article

   Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

   Insert

   PRMT9 (PRMT9 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Stop codons placed at aa 351 and 352 to create C …

   Promoter

   tac

   Available since

   July 14, 2016

   Availability

   Academic Institutions and Nonprofits only

4. NET33 pEGFP-N2 (594)

   Plasmid

   #61987

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   NET33 (SCARA5 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pFRT-TODestFLAGHA_RBPMS_KDR_K100A

   Plasmid

   #65663

   Purpose

   FLAG-HA expression vector with RBPMS carrying mutation K100A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

   Depositor

   Thomas Tuschl

   Article

   Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

   Insert

   RBPMS (RBPMS Human)
   

   Use

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   changed lysine 100 to alanine (KDR background)

   Promoter

   CMV, 2x Tet

   Available since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFRT-TODestFLAGHA_RBPMS_K36E_R38A_E39A

   Plasmid

   #65658

   Purpose

   FLAG-HA expression vector with RBPMS carrying mutation K36E, R38A, E39A

   Depositor

   Thomas Tuschl

   Article

   Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

   Insert

   RBPMS (RBPMS Human)
   

   Use

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   changed lysine 36 to glutamic acid, arginine 38 t…

   Promoter

   CMV, 2x Tet

   Available since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFRT-TODestFLAGHA_RBPMS_K36A_R38A_E39A

   Plasmid

   #65657

   Purpose

   FLAG-HA expression vector with RBPMS carrying mutation K36A, R38A, E39A

   Depositor

   Thomas Tuschl

   Article

   Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

   Insert

   RBPMS (RBPMS Human)
   

   Use

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   changed lysine 36 to alanine, arguing 38 to alani…

   Promoter

   CMV, 2x Tet

   Available since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFRT-TODestFLAGHA_RBPMS_KDR_F27A

   Plasmid

   #65661

   Purpose

   FLAG-HA expression vector with RBPMS carrying mutation F27A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

   Depositor

   Thomas Tuschl

   Article

   Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

   Insert

   RBPMS (RBPMS Human)
   

   Use

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   changed phenylalanine 27 to alanine (KDR backgrou…

   Promoter

   CMV, 2x Tet

   Available since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. DIRAS1

   Plasmid

   #55669

   Purpose

   expression of mouse DIRAS1

   Depositor

   Julian Downward

   Article

   Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

   Insert

   DIRAS1 (Diras1 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pSC-hXRCC1-K176R-IntE2

    Plasmid

    #52277

    Purpose

    Bacterial expression of human XRCC1-K176R C-terminally fused to GyrA intein, SUMO-E2 and GST

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    XRCC1 (XRCC1 Human)
    

    Use

    Tags

    GyrA-intein-Ubc9-GST

    Expression

    Bacterial

    Mutation

    K176R, SUMO acceptor site mutant

    Promoter

    T7

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHL1530

    Plasmid

    #37569

    Depositor

    Han Lim

    Article

    Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

    Inserts

    GFP
    TetR
    CsrA
    

    Use

    Synthetic Biology

    Tags

    glgC leader region

    Expression

    Bacterial

    Mutation

    start codon removed

    Promoter

    pConNoHind and pLlacO-1

    Available since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pHL1355

    Plasmid

    #37566

    Depositor

    Han Lim

    Article

    Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

    Inserts

    GFP
    TetR
    CsrA
    

    Use

    Synthetic Biology

    Tags

    glgC leader region

    Expression

    Bacterial

    Mutation

    start codon removed

    Promoter

    pConNoHind and pConNoHindM12

    Available since

    July 26, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pDSM-bc-Prps3-CAD-Tcyc1

    Plasmid

    #127728

    Purpose

    Yeast pathway position 3. CAD transcription unit with the RPS3 promoter and CYC1 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    cis-aconitate decarboxylase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Prps3

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDSM-bc-Peft2-CAD-Tklura3

    Plasmid

    #127729

    Purpose

    Yeast pathway position 3. CAD transcription unit with the EFT2 promoter and K. lactis URA3 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    cis-aconitate decarboxylase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Peft2

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDSM-bc-Psmtef1-CAD-Tyhi9

    Plasmid

    #127730

    Purpose

    Yeast pathway position 3. CAD transcription unit with the S. mikatae TEF1 promoter and YHI9 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    cis-aconitate decarboxylase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Psmtef1

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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16. MS2-adRNA (5'GAC)

    Plasmid

    #170127

    Purpose

    Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    MS2-ADAR2(5'GAC)-MS2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 and mouse U6

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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17. MS2-adRNA (3'GAC)

    Plasmid

    #170129

    Purpose

    Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    MS2-ADAR2(3'GAC)-MS2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 and mouse U6

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK5-HA-Ubiquitin-KO

    Plasmid

    #17603

    Purpose

    Mammalian expression of HA tagged ubiquitin carrying zero lysines (K0)

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    KO: all lysines mutated to arginines

    Promoter

    Available since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. LZ10 PBREBAC-H2BHalo

    Plasmid

    #91564

    Purpose

    piggybac based mammalian expression vector for histone H2B HaloTag protein. Uses the EF1 promoter, confers Amp resistance in bacteria, and carrying NeoR for mammalian selection.

    Depositor

    James Zhe Liu

    Article

    Li et al Elife. 2016 Aug 3;5. pii: e17056. doi: 1

    Insert

    histone H2B-HaloTag
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. RRL.sin.cPPT.SFFV/Flag-Firefly luciferase.IRES-puro.WPRE (MT05)

    Plasmid

    #139446

    Purpose

    Lentiviral vector genome to ectopically express Firefly luciferase

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    FLAG-tag Firefly luciferase
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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