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pCMV-mGold2s-Rab4a-C-7

Plasmid

#231773

Purpose

Visualization of endosome in mammalian cells using mGold2s

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s-Rab4a-C-7 (RAB4A Human)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(7CNW)RS

Plasmid

#222874

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Cyano-L-Tryptophan (7-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pM-mKate2

Plasmid

#228629

Purpose

Mammalian expression of mKate2 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mKate2

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pM-LSSmOrange

Plasmid

#228619

Purpose

Mammalian expression of LSSmOrange and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

LSSmOrange

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hGRN2

Plasmid

#213683

Purpose

Expresses human granulin-2 with an N-terminal twin-Strep-FLAG tag

Depositor

Thomas Kukar

Article

Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi

Insert

human Granulin-2

Use

AAV

Tags

twin-Strep-FLAG

Promoter

cytomegalovirus enhancer/chicken β-actin promoter

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pM-mBeRFP

Plasmid

#228620

Purpose

Mammalian expression of mBeRFP and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mBeRFP

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pM-mTFP1

Plasmid

#228621

Purpose

Mammalian expression of mTFP1 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mTFP1

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pM-CyOFP1

Plasmid

#228623

Purpose

Mammalian expression of CyOFP1 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

CyOFP1

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pM-mOrange2

Plasmid

#228627

Purpose

Mammalian expression of mOrange2 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mOrange2

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pM-mRuby3

Plasmid

#228628

Purpose

Mammalian expression of mRuby3 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mRuby3

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pIN3 (gpdA-cTerm-Citrine2-ptrA)

Plasmid

#188737

Purpose

Expresses Citrine2 for C-terminal tagging

Depositor

Michael Bromley

Article

Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

Insert

gdpA-Citrine2 PtrA

Use

Filamentous fungal expression

Tags

Citrine2

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pIN4 (gpdA-nTerm-Citrine2-ptrA)

Plasmid

#188738

Purpose

Expresses Citrine2 for N-terminal tagging

Depositor

Michael Bromley

Article

Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

Insert

gdpA-Citrine2 PtrA

Use

Filamentous fungal expression

Tags

Citrine2

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pIN5 (gpdA-cTerm-mTurqoise2-ptrA)

Plasmid

#188739

Purpose

Expresses mTurqoise2 for C-terminal tagging

Depositor

Michael Bromley

Article

Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

Insert

gdpA-mTurquoise PtrA

Use

Filamentous fungal expression

Tags

mTurquoise

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pIN6 (gpdA-nTerm-mTurqoise2-ptrA)

Plasmid

#188740

Purpose

Expresses mTurqoise2 for N-terminal tagging

Depositor

Michael Bromley

Article

Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

Insert

gdpA-mTurqoise2 PtrA

Use

Filamentous fungal expression

Tags

mGreenlantern

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG3-drAgo2D-A

Plasmid

#221734

Purpose

Expression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-A (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Mutation

D683A in active site

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG3-drAgo2Y-F

Plasmid

#221732

Purpose

Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2Y-F (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Mutation

Y680F in active site

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG3-drAgo2D-E

Plasmid

#221731

Purpose

Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-E (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Mutation

D651E in active site

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG3-drAgo2

Plasmid

#221730

Purpose

Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2 (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-hsAgo2

Plasmid

#221718

Purpose

Expression of human Ago2 in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2 (AGO2 Human)

Use

Fish expression

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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PtetA-PBAD-PLacO3O1 200bp with pBELO_L6-PT3-T4_CAMR_TAN+

Plasmid

#225649

Purpose

tetA, araBAD and LacO3O1 promoters in tandem conformation with 200bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

Depositor

Andre Sanches Ribeiro

Article

Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

Insert

tetR/tetA, araBAD and LacO3O1 in tandem conformation

Tags

mCherry

Expression

Bacterial

Promoter

tetA, araBAD and LacO3O1 promoters

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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