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DNA-PKcs T3950D

Plasmid

#83320

Purpose

DNA-PKcs T3950A

Depositor

Katheryn Meek

Article

Douglas et al Mol Cell Biol. 2007 Mar;27(5):1581-91. E

Insert

human DNA-PKcs (PRKDC Human)

Expression

Mammalian

Mutation

Activation loop phosphorylation site 3950 substit…

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3xFLAG-V5-ccdB

Plasmid

#87064

Purpose

Mammalian Gateway-compatible expression vector backbone with an N-terminal 3xFLAG-V5 tag

Depositor

Susan Lindquist, Mikko Taipale

Article

Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

Type

Empty backbone

Tags

3xFLAG-V5

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ2817 pPB: CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

Plasmid

#84239

Purpose

Expresses ABA-inducible VPR-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

ABI-tagBFP-Sp dCas9
PYL1-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RIPK1

Plasmid

#78834

Purpose

To overexpress RIPK1 in Mammalian Cell

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK1 (RIPK1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA1-2XPP7

Plasmid

#75390

Purpose

sgRNA1-2XPP7

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-2XPP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pORTMAGE-3

Plasmid

#72678

Purpose

Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.

Depositor

Csaba Pál

Article

Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

Insert

mutL E32K

Tags

none

Expression

Bacterial

Mutation

E32K mutation conferring dominant mutator phenoty…

Available Since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-mOXT-hM3Dq-mCherry-WPRE

Plasmid

#70717

Purpose

Expresses DREADDs Gq receptor under oxytocin promoter control

Depositor

Daniel Geschwind

Article

Penagarikano et al Sci Transl Med. 2015 Jan 21;7(271):271ra

Insert

hM3Dq-mCherry

Use

AAV

Tags

mCherry

Promoter

mouse oxytocin

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pRetroX-Tight-MCS_PGK-GpNLuc

Plasmid

#70185

Purpose

Constiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

Depositor

Antonio Amelio

Article

Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-Nrf2

Plasmid

#67636

Purpose

AAV vector expressing Nrf2 gene

Depositor

Connie Cepko

Article

Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

Insert

Nrf2 (Nfe2l2 Mouse)

Use

AAV

Promoter

CMV

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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CAG-FLEx(FRT)-TC

Plasmid

#67827

Purpose

Flp-dependent expression of TVA-mCherry fusion to mediate modifed rabies virus expression in cells

Depositor

Liqun Luo

Article

Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

Insert

TVA-mCherry fusion

Use

AAV

Expression

Mammalian

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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