Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 171,442 results for: addgene

Showing: 11641 - 11660 of 171442 results

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PX459_NRF2-exon5-2

Plasmid

#177782

Purpose

sgRNA for CRISPR/Cas9-mediated deletion of human NRF2

Depositor

Michael Ristow

Article

Thomas et al Nat Commun. 2023 Dec 8;14(1):8142. doi:

Insert

NRF2 (NFE2L2 Human)

Use

CRISPR

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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iPEmaxΔR-D10A

Plasmid

#239381

Purpose

For inverse prime editor using nCas9-D10A-R221K-N394K and M-MLV-RTΔRNase H in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:

Insert

nCas9(D10A-R221K-N394K), M-MLV-RT(ΔRNase H)

Use

CRISPR

Tags

BPNLS and SV40 NLS, c-myc NLS

Expression

Mammalian

Mutation

D10A, R221K, N394K in Cas9; ΔRNase H in M-MLV RT

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_CMV-RFP-p2A-DAAO(R285A)-NES

Plasmid

#238921

Purpose

Expression of mutated DAAO(R285A) with a nuclear export signal and RFP as a reporter in mammalian cells

Depositor

Vsevolod Belousov

Article

Kalinichenko et al Redox Biol. 2023 Apr;60:102604. doi: 10.

Insert

RFP-p2A-DAAO(R285A)-NES

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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hNSE-NF186(K519TAG)-HA

Plasmid

#239782

Purpose

Expresses rat NF186 with a TAG codon at position 519 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K519TAG

Promoter

hNSE (obtained from Addgene plasmid #11606)

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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hNSE-NF186(K534TAG)-HA

Plasmid

#239783

Purpose

Expresses rat NF186 with a TAG codon at position 534 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K534TAG

Promoter

hNSE (obtained from Addgene plasmid #11606)

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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hNSE-NF186(K571TAG)-HA

Plasmid

#239784

Purpose

Expresses rat NF186 with a TAG codon at position 571 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K571TAG

Promoter

hNSE (obtained from Addgene plasmid #11606)

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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hNSE-NF186(K604TAG)-HA

Plasmid

#239785

Purpose

Expresses rat NF186 with a TAG codon at position 604 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K604TAG

Promoter

hNSE (obtained from Addgene plasmid #11606)

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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hNSE-NF186(K680TAG)-HA

Plasmid

#239786

Purpose

Expresses rat NF186 with a TAG codon at position 680 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K680TAG

Promoter

hNSE (obtained from Addgene plasmid #11606)

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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hNSE-NF186(K809TAG)-HA

Plasmid

#239787

Purpose

Expresses rat NF186 with a TAG codon at position 809 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K809TAG

Promoter

hNSE (obtained from Addgene plasmid #11606)

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBS-GMR-eya(shRNA)_EcoRImut

Plasmid

#240222

Purpose

pBS-GMR-eya(shRNA) vector with one of two EcoRI sites mutated (T->A)

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Type

Empty backbone

Expression

Insect

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-ECS-dSpyCatcher003

Plasmid

#234963

Purpose

Cre-dependent ECS-facing destabilized SpyCatcher003 (facing the extracellular space)

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

ECS-dSC

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

None

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-IgKL-SpyCatcher003-GINKO2

Plasmid

#234544

Purpose

to express a potassium sensor tagged with SpyCatcher in the liver

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

IgKL-SpyCatcher003-GINKO2

Use

AAV

Mutation

None

Promoter

P3

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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EHMT1_exon 3_gRNA

Plasmid

#228809

Purpose

CRISPR targeting of human EHMT1

Depositor

Shravanti Rampalli

Article

Biligiri et al PLoS Biol. 2024 Nov 7;22(11):e3002871. d

Insert

gRNA targeting EHMT1 exon 3 (EHMT1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.RMacL5ET-12.EGFP.BCC6.WPRE

Plasmid

#229638

Purpose

L5 extratelencephalic projection neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Article

He et al Neuron. 2025 May 21;113(10):1548-1561.e8

Insert

RMacL5ET-12.EGFP.BCC6

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.RMacL3-05.EGFP.BCB2.WPRE

Plasmid

#229620

Purpose

L3 pyramidal neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Article

He et al Neuron. 2025 May 21;113(10):1548-1561.e8

Insert

RMacL3-05.EGFP.BCB2

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.RMacL3-07.EGFP.BCB4.WPRE

Plasmid

#229622

Purpose

L3 pyramidal neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Article

He et al Neuron. 2025 May 21;113(10):1548-1561.e8

Insert

RMacL3-07.EGFP.BCB4

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.RMacL3-09.EGFP.BCB7.WPRE

Plasmid

#229624

Purpose

L3 pyramidal neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Article

He et al Neuron. 2025 May 21;113(10):1548-1561.e8

Insert

RMacL3-09.EGFP.BCB7

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.RMacL3-10.EGFP.BCB8.WPRE

Plasmid

#229625

Purpose

L3 pyramidal neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Article

He et al Neuron. 2025 May 21;113(10):1548-1561.e8

Insert

RMacL3-10.EGFP.BCB8

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.RMacL3-11.EGFP.BCB12.WPRE

Plasmid

#229626

Purpose

L3 pyramidal neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Article

He et al Neuron. 2025 May 21;113(10):1548-1561.e8

Insert

RMacL3-11.EGFP.BCB12

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.RMacL3-12.EGFP.BCC3.WPRE

Plasmid

#229627

Purpose

L3 pyramidal neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Article

He et al Neuron. 2025 May 21;113(10):1548-1561.e8

Insert

RMacL3-12.EGFP.BCC3

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 11641 - 11660 of 171442 results

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