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MX01

Plasmid

#158624

Purpose

GATEWAY and BacMam compatible mammalian expression vector with eGFP expression reporter (eGFP is expressed by same promotor but as a separate polypeptide chain).

Depositor

Timm Maier

Article

Scaiola et al Sci Adv. 2020 Nov 6;6(45). pii: 6/45/eab

Type

Empty backbone

Use

Gateway destination vector ; bacmam acceptor vect…

Tags

P2A

Expression

Mammalian

Promoter

CMV-IE

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pDN0604 (pDEST, HygR)

Plasmid

#210483

Purpose

pDEST vector with HygR marker for gene expression in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDN0603 (pDEST, NatR)

Plasmid

#210482

Purpose

pDEST vector with NatR marker for gene expression in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pDGE474

Plasmid

#153265

Purpose

M1 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE475

Plasmid

#153266

Purpose

M2 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta1

Plasmid

#106114

Purpose

Lentiviral expression of PRKRA delta1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Use

Lentiviral

Tags

DDK (FLAG) and myc

Mutation

AA 100-313

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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Gma-pCAMBIA-1301-STTM171

Plasmid

#84080

Purpose

Disturb the expression of miR171 in soybean

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR171

Expression

Bacterial, Plant, and Yeast…

Promoter

2x35S

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pMpGWB427

Plasmid

#68692

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pCB-CBEv1

Plasmid

#221138

Purpose

Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 1 without sgRNA construct. Expresses 3xF-HF-BE3.

Depositor

Craig Roy

Article

Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

Insert

3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)

Use

CRISPR

Tags

3xFLAG

Expression

Bacterial

Promoter

lacIq-Ptac

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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LAMP-OeNL_pcDNA3

Plasmid

#89525

Purpose

Orange color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Tags

LAMP1

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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PROM5_35Sx2

Plasmid

#136112

Purpose

Strong promoter that drives expression everywhere in Mp gemmae but much weaker in the notches

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

PROM_35Sx2 (CaMV)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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PROM5_35S

Plasmid

#136111

Purpose

Strong promoter that drives expression everywhere in Mp gemmae but much weaker in the notches

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

PROM_35S (CaMV)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pLD-puro-CnVA

Plasmid

#24587

Depositor

Jason Moffat

Article

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VA

Expression

Mammalian

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
pDEST-hPDI

Plasmid

#60555

Purpose

enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

Depositor

Han Choe

Article

Song et al Appl Biochem Biotechnol. 2013 May;170(1)

Type

Empty backbone

Tags

His6-hPDI

Expression

Bacterial

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pMpGWB227

Plasmid

#68618

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pR6Kg-Omega1

Plasmid

#165864

Purpose

Omega level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMVS-V1

Plasmid

#183094

Purpose

Basic conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicus

Depositor

Lars Angenent, Bastian Molitor

Article

Fink et al mBio. 2021 Dec 21;12(6):e0276621. doi: 1

Inserts

pME2001
thermostable neomycin phosphotransferase gene

Use

Bacterial propagation and conjugative

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-Cas9-tdTomato

Plasmid

#141013

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Cas9

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pMET7-GAG-GW

Plasmid

#194634

Purpose

Gateway destination vector for mammalian expression of Virotrap bait constructs

Depositor

Sven Eyckerman

Article

Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

Insert

p55 GAG

Tags

PQS1

Expression

Mammalian

Promoter

SRalpha

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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MTK234_032

Plasmid

#123995

Purpose

Encodes the spCas9 sgRNA1 hAAVS1 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA1 -hAAVS1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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