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pWZ414-F55

Plasmid

#23079

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 9 to Arginine, H3 K9R …

Available Since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-P41nmt1-mEGFP

Plasmid

#105140

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

Insert

kanMX6-P41nmt1-mEGFP

Tags

41nmt1-mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

41nmt1

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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CMYA5/pACT2

Plasmid

#97208

Purpose

CMYA5 (aa 4039 – 4069) in a GAL4 AD vector, used for yeast-two hybrid

Depositor

Rita Shiang

Article

Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

Insert

CMYA5 (CMYA5 Human)

Expression

Yeast

Available Since

Aug. 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pRS406 ATG1

Plasmid

#166846

Purpose

Expresses Atg1 with ATG1 3'UTR in yeasts.

Depositor

Daniel Klionsky

Article

Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

Insert

ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Promoter

ATG1 Promoter

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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A102 ΔAmpH Δ2x+ve

Plasmid

#129484

Purpose

Expression of Glo3 ΔAmpH lacking the COPI-binding motif in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

ADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)

Tags

His and MBP

Expression

Bacterial

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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A102-GAP-BoCCS-GRM

Plasmid

#123286

Purpose

Expression of Glo3 ΔAmpH (1-459) in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

ADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)

Tags

His and MBP

Expression

Bacterial

Promoter

T5

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pJT23_GalL_BE-PAPAP

Plasmid

#143486

Purpose

Expresses BE-PAPAP in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

BE-PAPAP

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation

Plasmid

#58830

Purpose

Expresses GFP-Tagged and mutated Osh2 PH domain in yeast

Depositor

Tim Levine

Article

Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

Insert

2 x Osh2 PH domain (OSH2 Budding Yeast)

Tags

GFP and Myc

Expression

Yeast

Mutation

changed Lysine 307 to Glutamate and Arginine 309 …

Promoter

PHO5

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-P3nmt1-mEGFP

Plasmid

#105137

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

Insert

kanMX6-P3nmt1-mEGFP

Tags

3nmt1-mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

3nmt1

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pLYS1- DdMCU_AIIA, Cterm Flag

Plasmid

#253538

Purpose

Expresses C-terminal Flag tagged DdMCU_AIIA

Depositor

Vamsi Mootha

Article

Kovacs-Bogdan et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

DdMCU (DDB_G0286429 Dictyostelium discoideum)

Use

Lentiviral

Tags

Flag

Mutation

changed D192A and E195

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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