164,839 results

1. pLPCX mito roGFP2-Orp1

   Plasmid

   #64992

   Purpose

   Mammalian expression of mitochondrial roGFP2-Orp1 (retroviral vector)

   Depositor

   Tobias Dick

   Article

   Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Use

   Retroviral

   Tags

   roGFP2

   Expression

   Mammalian

   Mutation

   mitochondrial targeting sequence

   Promoter

   Available Since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-HA-UBE3A-IRES-Puro

   Plasmid

   #107349

   Purpose

   Expresses HA tagged UBE3A in mammalian cells.

   Depositor

   Robert Sobol

   Article

   Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

   Insert

   UBE3A (UBE3A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 23, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pLexM-SLC7A11

   Plasmid

   #197993

   Purpose

   Expresses SLC7A11 with an N-terminal FLAG tag in mammalian cells

   Depositor

   Simon Newstead

   Article

   Parker et al Nat Commun. 2021 Dec 8;12(1):7147. doi:

   Insert

   SLC7A11 (SLC7A11 Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBT44c

   Plasmid

   #122600

   Purpose

   BE PACE selection, express C-intein-Cas9-ugi fusion

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   intein-dCas9-ugi fusion
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR_SFFV_H2B_miRFP703

   Plasmid

   #231951

   Purpose

   Lentiviral vector for constitutive expression of the far-red fluorescent protein miRFP703 fused with the H2B nuclear localization tag (SFFV promoter)

   Depositor

   Leonardo Morsut

   Insert

   H2B_miRFP703
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-5'-ENGRAM

   Plasmid

   #179157

   Purpose

   piggyBac plasmid for 5' ENGRAM recorder

   Depositor

   Jay Shendure

   Article

   Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   minP

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF6a-CD28-PafA

   Plasmid

   #113400

   Purpose

   Expresses CD28-PafA in mammalian cell

   Depositor

   Min Zhuang

   Article

   Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

   Insert

   CD28-PafA
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAVS1P-iCLHN

   Plasmid

   #66579

   Purpose

   AAVS1 safe harbor gene targeting donor expressing CAG-driven Nanoluc-HaloTag, compatible with pZT-AAVS1 TALENs

   Depositor

   Jizhong Zou

   Article

   Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

   Insert

   Nanoluc-HaloTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSET mNG-GECO1

   Plasmid

   #201933

   Purpose

   Expresses mNG-GECO1 indicator in bacteria

   Depositor

   Robert Campbell

   Article

   Zarowny et al ACS Sens. 2020 Jul 24;5(7):1959-1968. do

   Insert

   mNeonGreenGECO1
   

   Use

   Tags

   HIS tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1-shLacZ-1735

    Plasmid

    #115454

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shLacZ-1735
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. ERmoxGFP

    Plasmid

    #68072

    Purpose

    mammalian expression of ER localized moxGFP

    Depositor

    Erik Snapp

    Article

    Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

    Insert

    moxGFP
    

    Use

    Tags

    KDEL ER retention sequence and bovine prolactin s…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. SIV_Gag_Pol

    Plasmid

    #236243

    Purpose

    3rd generation SIV-based lentiviral packaging plasmid; Contains SIV Gag and Pol

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    SIV Gag and Pol
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-IRES2-p75NTR-GFP

    Plasmid

    #182947

    Purpose

    To express the rat p75NTR neurotrophin receptor. The gene contains an HA tag at the N-Terminal

    Depositor

    Pantelis Tsoulfas

    Insert

    p75NTR-mGFP
    

    Use

    Tags

    HA tag and mGFP

    Expression

    Mammalian

    Mutation

    The leader sequence is from preprotrypin

    Promoter

    CAG

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-Puro-TDP-43-A315T

    Plasmid

    #133755

    Purpose

    Lentiviral expression of human TDP-43 A315T disease mutant in mammalian cells.

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed alanine 315 to threonine

    Promoter

    CMV

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLentiCRISPRv2-STING_gRNA3

    Plasmid

    #127640

    Purpose

    Knock-out of human STING

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRetrosuper USP28 shRNA-1

    Plasmid

    #15663

    Depositor

    Martin Eilers

    Article

    Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

    Insert

    USP28 shRNA (USP28 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 2, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. Str-KDEL_SBP-EGFP-Ecadherin

    Plasmid

    #65286

    Purpose

    synchronize trafficking of Ecadherin from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ecadherin
    

    Use

    Tags

    EGFP and IL-2 Signal Sequence and Streptavidin Bi…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221_SLC7A5

    Plasmid

    #132197

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC7A5 (SLC7A5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 28, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pET-29b(+)_His-CBD-SpEndoH

    Plasmid

    #218196

    Purpose

    Bacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoH

    Depositor

    Florence Pojer

    Insert

    EndoH
    

    Use

    Tags

    tufA-His-CBD

    Expression

    Bacterial

    Mutation

    codon optimized Ecoli, residues 45-313

    Promoter

    T7

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAPM-D4 miR30-TRIM28 ts3

    Plasmid

    #115864

    Purpose

    TRIM28 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    TRIM28 shRNA (TRIM28 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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