We narrowed to 19,744 results for: Cre

1. pInducer20 2xHA GPR153

   Plasmid

   #226812

   Purpose

   Tet-inducible lentiviral vector for 2xHA GPR153 expression

   Depositor

   Ramiro Iglesias-Bartolome

   Article

   Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

   Insert

   GPR153 (GPR153 Human)
   

   Use

   Lentiviral

   Tags

   2xHA tag followed by a GDPPVAT linker

   Mutation

   Sequence optimized from NP_997253.2

   Available Since

   May 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. RCAS-Flag-BRAFV600E

   Plasmid

   #126615

   Purpose

   Retroviral expression of Flag tagged human oncogenic BRAFV600E

   Depositor

   Yi Li

   Article

   Bu et al Cancer Res. 2019 Jan 1;79(1):61-71. doi:

   Insert

   BRAF (BRAF Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   V600E

   Available Since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. CXCR5-DuET

   Plasmid

   #213222

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CXCR5 (CXCR5 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV-Bhlhe40-3xFLAG-IRES-BFP

   Plasmid

   #117263

   Purpose

   Retroviral overexpression of Bhlhe40 with a 3xFLAG

   Depositor

   Sarah Teichmann

   Article

   Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

   Insert

   Bhlhe40 (Bhlhe40 Mouse)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. EF1a_RUNX1_P2A_Hygro_Barcode

   Plasmid

   #120475

   Purpose

   Barcoded lentiviral vector to express RUNX1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   RUNX1 (RUNX1 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOGS539_haPD-1

   Plasmid

   #226716

   Purpose

   Plasmid enabling yeast-mediated expression and secretion of high affinity PD-1 microbody (haPD-1)

   Depositor

   Gautam Dantas

   Article

   Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

   Insert

   High affinity PD-1 microbody
   

   Tags

   HA tag and Mating factor alpha secretion signal

   Expression

   Yeast

   Promoter

   pTEF1

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOGS539_rPD-L1nb

   Plasmid

   #226717

   Purpose

   Plasmid enabling yeast-mediated expression and secretion of recombinant PD-L1 nanobody (rPD-L1nb)

   Depositor

   Gautam Dantas

   Article

   Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

   Insert

   Recombinant PD-L1 nanobody
   

   Tags

   HA tag, Histidine tag, and Mating factor alpha se…

   Expression

   Yeast

   Promoter

   pTEF1

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. EF1a_MYC deltaCTD_P2A_Hygro_Barcode

   Plasmid

   #120511

   Purpose

   Barcoded lentiviral vector to express MYC deltaCTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   MYC deltaCTD (MYC Human)
   

   Use

   Lentiviral

   Mutation

   Deletion of carboxy-terminal domain

   Promoter

   EF1a

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. EF1a_MYC deltaNTD_P2A_Hygro_Barcode

   Plasmid

   #120510

   Purpose

   Barcoded lentiviral vector to express MYC deltaNTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   MYC deltaNTD (MYC Human)
   

   Use

   Lentiviral

   Mutation

   Deletion of amino-terminal domain

   Promoter

   EF1a

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. EF1a_MYC deltaMBI_P2A_Hygro_Barcode

    Plasmid

    #120504

    Purpose

    Barcoded lentiviral vector to express MYC deltaMBI in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC deltaMBI (MYC Human)
    

    Use

    Lentiviral

    Mutation

    Deletion of MYC Box I domain

    Promoter

    EF1a

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. EF1a_MYC deltab_P2A_Hygro_Barcode

    Plasmid

    #120507

    Purpose

    Barcoded lentiviral vector to express MYC deltab in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC deltab (MYC Human)
    

    Use

    Lentiviral

    Mutation

    Deletion of basic motif

    Promoter

    EF1a

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. EF1a_MYC deltaMBII_P2A_Hygro_Barcode

    Plasmid

    #120505

    Purpose

    Barcoded lentiviral vector to express c-MYC deltaMBII in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    c-MYC deltaMBII (MYC Human)
    

    Use

    Lentiviral

    Mutation

    Deletion of MYC Box II domain

    Promoter

    EF1a

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCas-MmdCas12m-CBE1

    Plasmid

    #192284

    Purpose

    MmdCas12m-CBE1 expressed under a constitutive promoter

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    MmdCas12m-CDA-UGI
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D485A

    Promoter

    PJ23108

    Available Since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. EF1a_KLF6_P2A_Hygro_Barcode

    Plasmid

    #120492

    Purpose

    Barcoded lentiviral vector to express KLF6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    KLF6 (KLF6 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV.EF1α.mEmerald.CD9.miR9T

    Plasmid

    #170452

    Purpose

    Lentiviral plasmid that encodes mEmerald-CD9 fusion protein with microRNA 9 tandem cassette to restrict expression to microglia with EF1-alpha promoter.

    Depositor

    Tsuneya Ikezu

    Article

    Clayton et al Mol Neurodegener. 2021 Mar 22;16(1):18.

    Insert

    CD9 (CD9 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Promoter

    EF1a

    Available Since

    May 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKHH030_sgCtrl#1

    Plasmid

    #174142

    Purpose

    Lentiviral vector expressing a control sgRNA that targets a safe harbor site

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. EDNRB-DuET

    Plasmid

    #213233

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    EDNRB (EDNRB Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.1 puro CXCR4 siRNA-1

    Plasmid

    #12271

    Depositor

    Bob Weinberg

    Article

    Orimo et al Cell. 2005 May 6. 121(3):335-48.

    Insert

    CXCR4 siRNA (CXCR4 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. CRHR2-DuET

    Plasmid

    #213216

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CRHR2 (CRHR2 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGGS-SARS2-S-G614 (C-flag)

    Plasmid

    #156421

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (G614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

    Depositor

    Hyeryun Choe, Michael Farzan

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS CoV2)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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