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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 21,719 results for: ESC;

Showing: 11761 - 11780 of 21719 results

  1. AA054

    Plasmid
    #215932
    Purpose
    Fragmid fragment: (guide cassette) guide expression; reverse orientation
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    rev{U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v3}
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215932-plasmid-map-sequence-id-431474

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  2. AA183

    Plasmid
    #216025
    Purpose
    Fragmid fragment: (Cas protein) for prime editing; R221K;N394K for increased efficiency
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    nCas9 (H840A)_v2.1 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216025-plasmid-map-sequence-id-431472

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  3. AA285

    Plasmid
    #215947
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; sgCD81_v1; trRNA_v4 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215947-plasmid-map-sequence-id-431462

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  4. AA023

    Plasmid
    #215931
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; sgCD81_v1 [Sp]; trRNA_v2 [Sp]; CS2
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215931-plasmid-map-sequence-id-431459

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  5. AA102

    Plasmid
    #215935
    Purpose
    Fragmid fragment: (guide cassette) guide expression; reverse orientation; enables iBar UMIs
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    rev{U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v5}
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215935-plasmid-map-sequence-id-431435

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  6. AA107

    Plasmid
    #215936
    Purpose
    Fragmid fragment: (guide cassette) guide expression; enables iBar UMIs
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v5
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215936-plasmid-map-sequence-id-431416

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  7. pMV_AA091

    Plasmid
    #216106
    Purpose
    CRISPRa, gRNA expression with modified trRNA to recruit activators via PP7 tags (guide only)
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Insert
    gRNA expression with modified trRNA to recruit activators via PP7 tags
    Use
    CRISPR and Lentiviral; Assembled vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216106-plasmid-map-sequence-id-431410

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  16. pLVX-mCherry-SPASTIN, L177D

    Plasmid
    #180649
    Purpose
    Lentiviral expression vector for SPASTIN. Has L177D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-45.
    Depositor
    Wesley Sundquist
    Article
    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75
    Insert
    SPASTIN
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Starts at M87, L177D mutation, has siRNA resistan…
    Promoter
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    180649-plasmid-map-sequence-id-360019

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Showing: 11761 - 11780 of 21719 results