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pHAGE-MAP3K4

Plasmid

#116759

Purpose

Lentiviral expression of MAP3K4

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MAP3K4 (MAP3K4 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-G719C

Plasmid

#116254

Purpose

Lentiviral expression of EGFR G719C

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

G719C

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERBB3-D297Y

Plasmid

#116360

Purpose

Lentiviral expression of ERBB3 D297Y

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB3 (ERBB3 Human)

Use

Lentiviral

Mutation

D297Y

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_ZBTB24_WT_V5

Plasmid

#83004

Purpose

Gateway Donor vector containing ZBTB24, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ZBTB24 (ZBTB24 Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-A289T

Plasmid

#116229

Purpose

Lentiviral expression of EGFR A289T

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

A289T

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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PLEX-PGK-ANXA11-mCerulean

Plasmid

#164214

Purpose

pLEX lentivirus backbone expresses mCerulean tagged ANXA11 under PGK promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mCerulean

Promoter

PGK

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEx-iSeroSnFR

Plasmid

#128486

Purpose

Fluorescent reporter for serotonin (conditional + membrane localization tag)

Depositor

Lin Tian

Article

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Has Service

AAV9

Insert

iSeroSnFR

Use

AAV

Tags

Ig-kappa leader, Myc, PDGFR, and cpsfGFP

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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P521_VSX2_p2A-h2b_mRuby3

Plasmid

#239104

Purpose

Used in CRISPR gene editing to Insert a p2A-h2b_mRuby3 sequence before the stop codon of the endogenous human VSX2 gene

Depositor

Karl Wahlin

Article

Jones et al Cells. 2022 Oct 28;11(21):3412. doi: 10.

Insert

VSX2 (VSX2 Human)

Use

CRISPR

Tags

-p2A-h2b-mRuby3

Expression

Mammalian

Promoter

Endogenous VSX2 promoter

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pdest METTL1-CM Neo

Plasmid

#223061

Purpose

METTL1-CM gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1-CM (METTL1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

160-163 (LFPD>AFPA)

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-EGR3-P2A-mCherry

Plasmid

#203861

Purpose

Tet-inducible lentiviral plasmid expressing EGR3-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

EGR3 (EGR3 Human)

Expression

Mammalian

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-SMO

Plasmid

#116792

Purpose

Lentiviral expression of SMO

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

SMO (SMO Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PDGFRA-Y288C

Plasmid

#116453

Purpose

Lentiviral expression of PDGFRA Y288C

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PDGFRA (PDGFRA Human)

Use

Lentiviral

Mutation

Y288C

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMX-Flag-GFP_SLC45A1

Plasmid

#227951

Purpose

Co-localisation experiment

Depositor

Alessandro Ori

Article

Ghoochani et al Cell. 2026 Feb 5;189(3):765-782.e31. doi

Insert

SLC45A1 (SLC45A1 Human)

Use

Lentiviral

Tags

3xFLAG-EGFP

Expression

Mammalian

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-M766_A767insAI

Plasmid

#116279

Purpose

Lentiviral expression of EGFR M766_A767insAI

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

M766_A767insAI

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCDH-3_Flag-YTHDF2-D-MUT (nonsense mutation of sgYTHDF2-1)

Plasmid

#232947

Purpose

Expresses the domain-mutant YTHDF2 with synonymous mutations for resistance against sgYTHDF2-1-mediated knockdown

Depositor

Jianjun Chen

Article

Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

Insert

YTHDF2-D-MUT (YTHDF2 Human)

Use

Lentiviral

Mutation

5 site mutation (Y418A+D422A+W432A+W486A+W491A), …

Available Since

May 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-3_Flag-YTHDF2 (nonsense mutation of sgYTHDF2-1)

Plasmid

#232945

Purpose

Expresses YTHDF2 with synonymous mutations rendering it resistant to sgYTHDF2-1-mediated knockdown

Depositor

Jianjun Chen

Article

Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

Insert

YTHDF2 (YTHDF2 Human)

Use

Lentiviral

Mutation

synonymous mutation

Available Since

May 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-3_Flag-YTHDF2-m(6)A-MUT (nonsense mutation of sgYTHDF2-1)

Plasmid

#232946

Purpose

Expresses the m6A-binding-deficient mutant of YTHDF2 with synonymous mutations for resistance against sgYTHDF2-1-mediated knockdown

Depositor

Jianjun Chen

Article

Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

Insert

YTHDF2-m(6)A-MUT (YTHDF2 Human)

Use

Lentiviral

Mutation

2 site mutation (W432A+W486A), synonymous mutation

Available Since

May 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-ALK-R1275Q

Plasmid

#116111

Purpose

Lentiviral expression of ALK R1275Q

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ALK (ALK Human)

Use

Lentiviral

Mutation

R1275Q

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-MAP2K1-K57N

Plasmid

#116427

Purpose

Lentiviral expression of MAP2K1 K57N

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MAP2K1 (MAP2K1 Human)

Use

Lentiviral

Mutation

K57N

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_ARAF_WT

Plasmid

#82900

Purpose

Gateway Donor vector containing ARAF, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

NM_001654 (ARAF Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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