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Plasmid#73807PurposeExpresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in mammalian cells (and can be packaged as AAV)DepositorInsertPeredox
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFSW GFP IRES GFP
Plasmid#133815PurposeEncodes EGFP that can be used as a control for viral expressionDepositorTypeEmpty backboneUseLentiviralTagsIRES-GFPExpressionMutationPromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMBP-MS2
Plasmid#65104Purposecan be used to isolate RNA binding complexesDepositorInsertMS2
UseTagsMBPExpressionBacterialMutationdouble mutation (V75Q and A81G) that prevents oli…PromotertacAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPSup
Plasmid#149416PurposePlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted just upstream of the minimal promoter.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUbiGUSPlus
Plasmid#64402PurposeUbiquitin promoter driving synthetic optimised GUSPlus gene. Can be used as a reporter of promoter activity in plants by replacing Ubi promoter with sequence of interest.DepositorInsertubiquitin (Ubi-1) promoter
UseTagsGUSPlus reporterExpressionPlantMutationPromoterUbi-1Available sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6-Universal
Plasmid#52694PurposeThis plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.DepositorInsertsCas9
Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
UseCRISPR; Toxoplasma gondiiTags3X FLAG and Nuclear localization signalExpressionMutationPromoterTgTUB1 and Toxoplasma U6Available sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSKI
Plasmid#232484PurposepSKI can be used for single-copy transgene expression within the Universal SKI System or for extrachromosomal arrays. It contains an MCS with 47 single cuts and two synthetic 900 bp homology arms.DepositorInsertpSKI
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-mito
Plasmid#71542PurposeBiomedical ResearchDepositorInsertmyc-mito
UseCytomegalovirusTagsnoneExpressionMutationPromoterCMVAvailable sinceDec. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
PIA900
Plasmid#104401PurposePolycistronic vector for expression of E. coli core RNAPDepositorInsertrpoA, B, C, and Z
UseE. coli expression vectorTagsencodes a C-terminally His-tagged rpoC which can …ExpressionBacterialMutationPromoterT7Available sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV UBC APEX2-3xFLAG-IRES-eGFP
Plasmid#214983PurposeCytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).DepositorInsertAPEX2 (APX2 Glycine max (soybean))
UseLentiviralTags3xFLAGExpressionMutationPromoterUBCAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE-60NA-proinsulin-mEGFP
Plasmid#160464PurposeEncodes a fusion of human proinsulin and the monomeric enhanced EGFP. This construct can be used as a negative control for superfolder activity in bacteriaDepositorInsertproinsulin-mEGFP
UseTagsExpressionBacterialMutationPromoterT5Available sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
bRA77
Plasmid#100953PurposeGAL1 driven Cas9. gRNA can be cloned into BplI sites.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPSint
Plasmid#149421PurposePlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted into the IV2 intron in the GFP reporter gene.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a_LolT_C41S
Plasmid#211789PurposeExpression plasmid in E. coli BL21(DE3) , which can be used for expression and purification to get protein LolT_C41SDepositorInsertlolt mutant
UseTagsHis tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
bRA66
Plasmid#100952PurposeGAL1 driven Cas9. gRNA can be cloned into BplI sites.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pScDD2_ERdd
Plasmid#109047Purposedestabilizing domains for yeast (S. cerevisiae) derived from the human estrogen receptor ligand binding domain. This ERdd can be stabilized with 10 uM 4-hydroxytamoxifen (4-OHT, OHTAM).DepositorInsertER50 (ESR1 H. sapiens gene with yeast enhanced codon)
UseTagsyeast enhanced GFP with 8 amino acids TSGRGEGQ li…ExpressionYeastMutation3 mutations : E380G, R434G, N532SPromoterSOD1Available sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOTag-glmS-4H
Plasmid#106378PurposeCan be used as a PCR template to amplify the glmS-HA fragment for gene specific tagging and regulationDepositorInsertGlmS-3xHA
UseTagsExpressionMutationPromoterAvailable sinceMarch 2, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSWAP_Entry_HAIB
Plasmid#184869PurposeFor scarless integration into the chromosome, HAIB plasmid can be usedin place of the HA, INS and HB modules.DepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMB70
Plasmid#47943PurposeCan be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequencesDepositorInsertguide RNA
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZH516
Plasmid#102669PurposeConstitutive expression of GFPmut2; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only