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Pr1-T7RNAP

Plasmid

#67738

Purpose

OR2-OR1-Pr1 promoter expressing T7 RNAP

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-T7RNAP-T500

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

OR2-OR1-Pr1

Available since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 PSMC1

Plasmid

#67087

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

PSMC1 (PSMC1 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

E275G, I287V and R294G

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
GTL-g

Plasmid

#81099

Purpose

Gene Tagging vector LEU/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

sfGFP

Use

Cre/Lox

Tags

sfGFP

Expression

Yeast

Mutation

Promoter

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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YFP(1-158)-STOP

Plasmid

#66107

Purpose

This vector attaches YFP(1-158) to the C-terminus of a protein

Depositor

Catherine Berlot

Article

Hu et al J Biol Chem. 2013 Apr 19;288(16):11416-3

Insert

YFP(1-158)STOP

Use

Tags

Expression

Mammalian

Mutation

Gln69 was changed to Met.

Promoter

CMV

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pRUSH

Plasmid

#54479

Purpose

Mouse genomic ROSA26 targeting vector, expresses EGFP, U6promoter to drive short RNAs, pgkneo, cassette flanked by loxP sites

Depositor

Laurie Jackson-Grusby

Article

Brown et al Genesis. 2014 Jan;52(1):39-48. doi: 10.1

Type

Empty backbone

Use

Cre/Lox, Mouse Targeting, and RNAi

Tags

SA-EGFP-polyA, U6 promoter, loxP, and pgk-neo

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only
pBbdCas9S_Psyn-sgRNAflaB

Plasmid

#149642

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30, Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_Psyn-sgRNAmreB

Plasmid

#149653

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30, Psyn

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pMK1224

Plasmid

#84221

Purpose

Lentiviral expression of shRNAs, compatible with double-shRNA expression, SFFV promoter, BFP

Depositor

Jonathan Weissman, Martin Kampmann

Article

Kampmann et al Proc Natl Acad Sci U S A. 2015 Jun 30;11

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA

Plasmid

#133344

Purpose

for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets cpaA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_cpaA

Use

CRISPR

Tags

Expression

Mutation

Promoter

constitutive

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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Pr2-T7RNAP

Plasmid

#67739

Purpose

OR2-OR1-P2 promoter expressing T7 RNAP

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-T7RNAP-T500

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

OR2-OR1-Pr2

Available since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pISH-Galpha-o

Plasmid

#15930

Depositor

Peter Mombaerts

Article

Rodriguez et al Nat Neurosci. 2002 Feb . 5(2):134-40.

Insert

Galpha-o (Gnao1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only
pAN-PBAD-sgRNA-A2T

Plasmid

#62258

Purpose

expression of A2T sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A2T sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pHW-CIBN-MP-HRW-CRY2olig-VHH(GFP)

Plasmid

#126026

Purpose

express RFP-tagged LARIAT variant for optogenetic clustering

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CIBN-MP; CRY2 PHRolig-VHH(GFP)

Use

Tags

RFP

Expression

Insect

Mutation

CRY2 E490G

Promoter

Hsp70

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TCF3

Plasmid

#67110

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TCF3 (TCF3 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Truncated isoform

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pLL3.7 Mor2 shRNA

Plasmid

#59298

Purpose

3rd generation lentiviral vector expressing shRNA to mouse Opmr1

Depositor

Ulrike Heberlein

Article

Lasek et al Genes Brain Behav. 2007 Nov;6(8):728-35.

Insert

Mor2 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1-p19-T7-GagB500

Plasmid

#46314

Depositor

Judy Lieberman

Article

Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

Inserts

p19
GagB500 DNA-1
linker
GagB500 DNA-2

Use

Pro-sirna

Tags

6XHis and GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 YWHAB

Plasmid

#67063

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

YWHAB (YWHAB Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pAN-PBAD-sgRNA-A1T

Plasmid

#62256

Purpose

expression of A1T sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A1T sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pHW-CIBN-mCerulean-MP-HRW-CRY2-VHH(GFP)

Plasmid

#126025

Purpose

express RFP- and mCer-tagged LARIAT for optogenetic clustering

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CIBN-mCerulean-MP; CRY2 PHR-VHH(GFP)

Use

Tags

RFP

Expression

Insect

Mutation

WT

Promoter

Hsp70

Available since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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Pr-delRBS-deGFP

Plasmid

#67744

Purpose

Pr promoter expressing a deGFP that is missing a ribosomal binding site

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

deGFP-T500

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

OR2-OR1-Pr

Available since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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