We narrowed to 13,583 results for: TIM

1. pmNeonGreenHO-3xFLAG-G

   Plasmid

   #127914

   Purpose

   a mammalian expression backbone vector for humanized mNeonGreen-3xFlag

   Depositor

   Isei Tanida

   Article

   Tanida-Miyake et al PLoS One. 2018 Jan 17;13(1):e0191108. do

   Insert

   humanized mNeonGreen
   

   Use

   Tags

   mNeonGreen-3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO_LAMP1_HaloTag7

   Plasmid

   #175531

   Purpose

   Lyzosomal expression of HaloTag7 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   LAMP1-HaloTag7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFH54

   Plasmid

   #128183

   Purpose

   Level1 AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mCyRFP1-mMaroon

   Plasmid

   #84486

   Purpose

   Direct FRET pair Fusion mCyRFP1-mMaroon

   Depositor

   Ryohei Yasuda

   Article

   Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   mCyRFP1-mMaroon1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. COVID-SARS2 NSP16/10

   Plasmid

   #159615

   Purpose

   Bacterial co-expression vector fo Covid-SARS2 NSP16 and NSP10

   Depositor

   Opher Gileadi

   Inserts

   Covid-SARS2 Nsp16
   Covid-SARS2 Nsp10
   

   Use

   Tags

   His6-Zb-TEV and none

   Expression

   Bacterial

   Mutation

   Codon-optimized for E. coli expression

   Promoter

   (Bicistronic) and T7 - LacO

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. NLS-LgBiT-CaM

   Plasmid

   #183401

   Purpose

   Expresses nuclear-localized LgBiT-CaM

   Depositor

   Alice Ting

   Article

   Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

   Insert

   LgBiT-CaM
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET-51b-HaloTag10

   Plasmid

   #175520

   Purpose

   Expression of HaloTag10 in bacterial cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   HaloTag10
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   HaloTag10 = HaloTag7-Q165W

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEB1-T-Sapphire

   Plasmid

   #103977

   Purpose

   Plasmid encoding T-Sapphire

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   T-Sapphire
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (Q69M, C70V, …

   Promoter

   proC

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CIp10 - ffDronpa ATGme

   Plasmid

   #163118

   Purpose

   Expresses in Candida albicans

   Depositor

   Patrick Van Dijck

   Article

   Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

   Insert

   ffDronpa ATGme
   

   Use

   Synthetic Biology; Candida albicans expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GPI_2xmCherry

    Plasmid

    #127812

    Purpose

    Homo-dimer mCherry-mCherry expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

    Insert

    glycosylphosphatidylinositol-mCherry-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. BCJJ345

    Plasmid

    #117504

    Purpose

    YAO:Cas9:E9 Golden Gate Level 1 Position 2 reverse

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:tagt:YAO:Cas9-3(intron):E9:ttgc:BpiI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtYAO

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIBB-mEGFP-IRES-puro2b

    Plasmid

    #26462

    Depositor

    Daniel Gerlich

    Article

    Held et al Nat Methods. 2010 Sep . 7(9):747-54.

    Insert

    pIBB
    

    Use

    Tags

    mEGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5/FRT/TO_HaloTag7_SKL

    Plasmid

    #175532

    Purpose

    Peroxysomal expression of HaloTag7 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    HaloTag7-SKL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. beta2 (px330:2-guideCas9)

    Plasmid

    #169435

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMSCVII-BFP

    Plasmid

    #162755

    Purpose

    Retroviral (MSCV) expression of mTagBFP2 fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Insert

    mTagBFP2
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCIS.MTRIA-DA

    Plasmid

    #184603

    Purpose

    For expression of a fluorescent sensor for dopamine in mammalian cells

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-DA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pmTurquoise-N1

    Plasmid

    #60559

    Purpose

    Expresses the cyan fluorescent protein variant mTurquoise

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Methods. 2010 Feb;7(2):137-9. doi: 1

    Insert

    mTurquoise
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJKR-L-cdaR

    Plasmid

    #62558

    Purpose

    Glucarate biosensor. Low copy plasmid that expresses GFP proportional to glucarate concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pZE-PpadC-yveF-yveG-egfp

    Plasmid

    #201791

    Purpose

    the promoter can be recognized by PadR

    Depositor

    Yajun Yan

    Article

    Jiang et al ACS Synth Biol. 2021 Jan 15;10(1):132-14

    Insert

    PpadC-yveF-yveG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GluA3 (px330:2-guideCas9)

    Plasmid

    #169424

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJKR-L-acuR

    Plasmid

    #62568

    Purpose

    Acrylate biosensor. Low copy plasmid that expresses GFP proportional to acrylate concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJKR-L-mphR

    Plasmid

    #62560

    Purpose

    Macrolide biosensor. Low copy plasmid that expresses GFP proportional to macrolide concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1-HisFlag-CAD

    Plasmid

    #31615

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al Nucleic Acids Res. 2004 . 32(15):4524-3

    Insert

    CAD
    

    Use

    Tags

    3xFlag, His, and T7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

24. DL147_plenti_TRE_HT-iPAK4

    Plasmid

    #187447

    Purpose

    Tet-On inducible expression of HaloTag-iPAK4 in a lentiviral backbone

    Depositor

    Adam Cohen

    Article

    Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    INKA-box Pak4
    

    Use

    Lentiviral

    Tags

    halo tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEB1-mTurquoise2

    Plasmid

    #103974

    Purpose

    Plasmid encoding mTurquoise2

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mTurquoise2
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F64L, Y66W, …

    Promoter

    proC

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CS2 Flag-Smad3 (3A)

    Plasmid

    #14964

    Depositor

    Joan Massague

    Article

    Kretzschmar et al Genes Dev. 1999 Apr 1. 13(7):804-16.

    Insert

    Smad3 (3A) (SMAD3 Human)
    

    Use

    Xenopus/mammalian/avian/zebrafish

    Tags

    Flag

    Expression

    Mutation

    Three Ser to Ala mutations in the phosphorylation…

    Promoter

    Available since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEB1-mTurquoise

    Plasmid

    #103972

    Purpose

    Plasmid encoding mTurquoise

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mTurquoise
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F64L, Y66W, …

    Promoter

    proC

    Available since

    Dec. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEB1-mEmerald

    Plasmid

    #103982

    Purpose

    Plasmid encoding mEmerald

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mEmerald
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F64L, S65T, …

    Promoter

    proC

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pmTurquoise-C1

    Plasmid

    #60558

    Purpose

    Expresses the cyan fluorescent protein variant mTurquoise

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Methods. 2010 Feb;7(2):137-9. doi: 1

    Insert

    mTurquoise
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pYES2.1-pomSua1

    Plasmid

    #107446

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    Sua1
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    See depositor comments below.

    Promoter

    GAL1

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge