Addgene

We narrowed to 151,085 results for: Des

Showing: 2321 - 2360 of 151085 results

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Expression_vector_RO2_6

Plasmid

#158017

Purpose

Expression vector for the de novo designed protein Expression_vector_RO2_6

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO2_6

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector_RO2_9

Plasmid

#158018

Purpose

Expression vector for the de novo designed protein Expression_vector_RO2_9

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO2_9

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector_RO2_10

Plasmid

#158019

Purpose

Expression vector for the de novo designed protein Expression_vector_RO2_10

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO2_10

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector_RO2_15

Plasmid

#158020

Purpose

Expression vector for the de novo designed protein Expression_vector_RO2_15

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO2_15

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector_RO2_20

Plasmid

#158021

Purpose

Expression vector for the de novo designed protein Expression_vector_RO2_20

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO2_20

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector_RO2_25

Plasmid

#158022

Purpose

Expression vector for the de novo designed protein Expression_vector_RO2_25

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO2_25

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector_NT_1

Plasmid

#158023

Purpose

Expression vector for the de novo designed protein Expression_vector_NT_1

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein NT_1

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector_NT_8

Plasmid

#158024

Purpose

Expression vector for the de novo designed protein Expression_vector_NT_8

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein NT_8

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector_NT_9

Plasmid

#158025

Purpose

Expression vector for the de novo designed protein Expression_vector_NT_9

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein NT_9

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
Expression_vector_NT_10

Plasmid

#158026

Purpose

Expression vector for the de novo designed protein Expression_vector_NT_10

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein NT_10

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV CMV-DIO-(mCherry-U6)-shRNA(scrambled)

Plasmid

#214733

Purpose

Expresses mCherry protein in infected and cre positive cells

Depositor

Félix Leroy

Article

de Leon Reyes et al Cell. 2023 Sep 14;186(19):4152-4171.e31.

Insert

anti-Crh shRNA (scrambled) / mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-CMV-GCaMP3-373

Plasmid

#91779

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

Douglas Kim, GENIE Project

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

GCaMP3-373

Use

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-CMV-GCaMP3-44

Plasmid

#91780

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

Douglas Kim, GENIE Project

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

GCaMP3-44

Use

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pUDP010

Plasmid

#101166

Purpose

E. coli/S. cerevisiae amdS shuttle vector expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 in S. pastorianus (HH-gRNASeILV6-HDV)

Depositor

Jean-Marc Daran

Article

de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

Insert

HH-gRNA-HDV targetting SeILV6 in S. pastorianus

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

ScTDH3

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-U6-SA-WTmLdlrEx14-gRNA2-N22-HLP-SACas9-HA-OLLAS-spA

Plasmid

#206860

Purpose

An AAV plasmid with U6 promoter driving a gRNA against LDLR and liver specific HLP promoter driving SaCas9

Depositor

William Lagor

Article

De Giorgi et al Methods Mol Biol. 2022;2419:673-713. doi

Insert

mLdlr gRNA (Ldlr Mouse)

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
tet-pLKO.neo_shGFP

Plasmid

#110470

Purpose

Control, silence GFP gene, doxycycline inducible, neomycin selection

Depositor

Sandra Martha Gomes Dias

Article

Reis et al J Biol Chem. 2019 Jun 14;294(24):9342-93

Insert

GFP

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1/TO (RNA PolIII)

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-CMV-GCaMP6-150

Plasmid

#91777

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

Douglas Kim, GENIE Project

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

GCaMP6-150

Use

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV CMV-DIO-(mCherry-U6)-shRNA(anti-Crh)

Plasmid

#214732

Purpose

Expresses shRNA against CRH, marked with mCherry, in infected and cre positive cells

Depositor

Félix Leroy

Article

de Leon Reyes et al Cell. 2023 Sep 14;186(19):4152-4171.e31.

Insert

anti-Crh shRNA / mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-CMV-GCaMP6-210

Plasmid

#91778

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

Douglas Kim, GENIE Project

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

GCaMP6-210

Use

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pUDP012

Plasmid

#101167

Purpose

E. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)

Depositor

Jean-Marc Daran

Article

de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

Insert

HH-gRNA-HDV targetting SeILV6 in S. pastorianus

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

ScTDH3

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pUDP044

Plasmid

#101168

Purpose

pUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)

Depositor

Jean-Marc Daran

Article

de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

Insert

polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

ScTDH3

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBB-80

Plasmid

#8814

Depositor

Cynthia Wolberger

Article

Batchelor et al Science 1998 Feb 13;279(5353):1037-41.

Insert

GABPa1 (318-430) (Gabpa Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only
MIGS2.1 (FF570)

Plasmid

#35247

Depositor

Detlef Weigel

Article

de Felippes et al Plant J. 2011 Dec 28. doi: 10.1111/j.136

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MIGS5.1 (FF572)

Plasmid

#35253

Depositor

Detlef Weigel

Article

de Felippes et al Plant J. 2011 Dec 28. doi: 10.1111/j.136

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MIGS2.2 (FF573)

Plasmid

#35248

Depositor

Detlef Weigel

Article

de Felippes et al Plant J. 2011 Dec 28. doi: 10.1111/j.136

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MIGS1.1 (JW493)

Plasmid

#35245

Depositor

Detlef Weigel

Article

de Felippes et al Plant J. 2011 Dec 28. doi: 10.1111/j.136

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MIGS4.1 (FF571)

Plasmid

#35251

Depositor

Detlef Weigel

Article

de Felippes et al Plant J. 2011 Dec 28. doi: 10.1111/j.136

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MIGS1.2 (JW640)

Plasmid

#35246

Depositor

Detlef Weigel

Article

de Felippes et al Plant J. 2011 Dec 28. doi: 10.1111/j.136

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MIGS3.2 (FF574)

Plasmid

#35250

Depositor

Detlef Weigel

Article

de Felippes et al Plant J. 2011 Dec 28. doi: 10.1111/j.136

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MIGS3.1 (FF537)

Plasmid

#35249

Depositor

Detlef Weigel

Article

de Felippes et al Plant J. 2011 Dec 28. doi: 10.1111/j.136

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MIGS5.2 (FF576)

Plasmid

#35254

Depositor

Detlef Weigel

Article

de Felippes et al Plant J. 2011 Dec 28. doi: 10.1111/j.136

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MIGS4.2 (FF575)

Plasmid

#35252

Depositor

Detlef Weigel

Article

de Felippes et al Plant J. 2011 Dec 28. doi: 10.1111/j.136

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pFSW FLEx Synapsin-FLAG

Plasmid

#139653

Purpose

Conditional expression of Synapsin-FLAG

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

Synapsin-FLAG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEFS-ccdB-12X MS2_mPGK-Puro

Plasmid

#177809

Purpose

Assembly backbone for tagging RNA transcript with a 12X tandem MS2 stemloop at the 3'

Depositor

Sven Eyckerman

Article

Delhaye et al Sci Rep. 2022 Jan 27;12(1):1513. doi: 10

Type

Empty backbone

Use

Tags

12X MS2 stem loops

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFSW FLEx mCherry-KASH

Plasmid

#139652

Purpose

Conditional expression of mCherry-KASH

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

mCherry-KASH

Use

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBB542

Plasmid

#27395

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 . 7():32.

Insert

dnaK, dnaJ, groESL

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBB550

Plasmid

#27396

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 . 7():32.

Insert

dnaK, dnaJ, GroESL

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBB540

Plasmid

#27393

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 . 7():32.

Insert

grpE, clpB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 26, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBB528

Plasmid

#27390

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 . 7():32.

Insert

lacIq

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBB530

Plasmid

#27391

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 . 7():32.

Insert

grpE

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge

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