We narrowed to 9,223 results for: Coli

1. pKP2008

   Plasmid

   #167601

   Purpose

   Express ExbB (205-214 Ala),ExbD

   Depositor

   Kathleen Postle

   Insert

   ExbB, ExbD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   exbB gene with ala codons replacing codons 205-21…

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNeae2

   Plasmid

   #168300

   Purpose

   Bacterial surface display of polypeptides (nanobodies) in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Salema et al PLoS One. 2013 Sep 23;8(9):e75126. doi:

   Insert

   Enterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
   

   Use

   Tags

   6xHis, E-tag, and myc-tag

   Expression

   Bacterial

   Mutation

   Promoter

   LacI-Plac

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCH1/RAD51o

   Plasmid

   #102562

   Purpose

   Express Human RAD51 protein codon optimzed for E.coli and the E.coli GorE Operon

   Depositor

   Maria Spies

   Article

   Subramanyam et al Proc Natl Acad Sci U S A. 2016 Oct 11;11

   Inserts

   Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Synthetic, Human)
   E. coli groE operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli Expression

   Promoter

   T7

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSEVA228-pro4IUPi

   Plasmid

   #122018

   Purpose

   Expresses IUP pathway genes (ChK, IPK, idi) under constitutive promoter. Kan. resist.

   Depositor

   Gregory Stephanopoulos

   Article

   Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

   Inserts

   Choline kinase (CKI1 Budding Yeast)
   Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
   isopentenyl-diphosphate Delta-isomerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E. coli

   Promoter

   pro4

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-TRSter

   Plasmid

   #78865

   Purpose

   Expresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS element

   Depositor

   Elias Arnér

   Article

   Arner et al J Mol Biol. 1999 Oct 8;292(5):1003-16.

   Insert

   Thioredoxin Reductase (Txnrd1 Rat)
   

   Use

   Tags

   Mutated E. coli-type SECIS element (3’ end of ins…

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTrc99S-ssDsbA-MBP-4xDQNAT

   Plasmid

   #128398

   Purpose

   Periplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Maltose binding protein
   

   Use

   Tags

   4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

   Expression

   Bacterial

   Mutation

   Y342H- please see depositor comments

   Promoter

   trc

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-ITR-EcYtR-mCherry

   Plasmid

   #217362

   Purpose

   Expresses E. coli tyrosine tRNA and a wild-type mCherry reporter; can be packaged into AAV

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

   Insert

   E. coli tyrosine tRNA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCK301

   Plasmid

   #87767

   Purpose

   E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest to express using L-rhamnose, three terminators, ampR, pBR322 origin, lacI

   Depositor

   John Heap

   Article

   Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

   Insert

   PrhaBAD-sfGFP
   

   Use

   Synthetic Biology

   Tags

   6xHis Tag

   Expression

   Bacterial

   Mutation

   Promoter

   PrhaBAD rhamnose-inducible promoter from E. coli

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTET-IUPi

   Plasmid

   #122019

   Purpose

   Expresses IUP pathway genes (ChK, IPK, idi) under inducible promoter. aTC inducible. Kan. resist.

   Depositor

   Gregory Stephanopoulos

   Article

   Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

   Inserts

   Choline kinase (CKI1 Budding Yeast)
   Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
   isopentenyl-diphosphate Delta-isomerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E. coli

   Promoter

   pTET

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYihI-N-term D-N/E-Q

    Plasmid

    #233095

    Purpose

    Expression of GST-YihI with N-term aspartic acids (D) mutated to asparagine (N) and glutamic acids (E) mutated to glutamine (Q)

    Depositor

    Michael Downey

    Article

    Baijal et al bioRxiv 2025.02.12.637445

    Insert

    YihI with N-term aspartic acids (D) mutated to asparagine (N) and glutamic acids (E) mutated to glutamine (Q)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    N-term aspartic acids (D) mutated to asparagine (…

    Promoter

    Available since

    May 7, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pYihI-N-term D-A/E-L

    Plasmid

    #233097

    Purpose

    Expression of GST-YihI with N-term aspartic acids (D) mutated to alanine (A) and glutamic acids (E) mutated to leucine (L)

    Depositor

    Michael Downey

    Article

    Baijal et al bioRxiv 2025.02.12.637445

    Insert

    YihI with N-term aspartic acids (D) mutated to alanine (A) and glutamic acids (E) mutated to leucine (L)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    N-term aspartic acids (D) mutated to alanine (A) …

    Promoter

    Available since

    May 7, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pRAR051

    Plasmid

    #185029

    Purpose

    400 nt l31 promoter region, l31 5'UTR with nt. 47-51 and 80-84 mutated, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Nucleotides 47-51 & 80-84 mutated rpmE 5'…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRAR052

    Plasmid

    #185030

    Purpose

    400 nt l31 promoter region, l31 5'UTR with nt. 56-61 and 68-73 mutated, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Nucleotides 56-61 and 68-73 mutated in rpmE 5…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRAR104

    Plasmid

    #185034

    Purpose

    400 nt l31 promoter region, l31 5'UTR A74 and A76 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A74 and A76 deletion of rpmE 5'UTR, Only fir…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRAR105

    Plasmid

    #185035

    Purpose

    400 nt l31 promoter region, l31 5'UTR G52 and G79 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G52 and G79 deletion of rpmE 5'UTR, Only fir…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRAR107

    Plasmid

    #185037

    Purpose

    400 nt l31 promoter region, l31 5'UTR AGA74-76GAG mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AGA74-76GAG mutation of rpmE 5'UTR, Only fir…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRAR109

    Plasmid

    #185038

    Purpose

    400 nt l31 promoter region, l31 5'UTR G60 and C69 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G60 and C69 deletion of rpmE 5'UTR, Only fir…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRAR110

    Plasmid

    #185039

    Purpose

    400 nt l31 promoter region, l31 5'UTR G62 and G67 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G62 and G67 deletion of rpmE 5'UTR, Only fir…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRAR039

    Plasmid

    #185025

    Purpose

    400 nt l31 promoter region, l31 5'UTR with nt. 1-31 deleted, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Nucleotides 1-31 deleted from rpmE 5'UTR, On…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRAR040

    Plasmid

    #185026

    Purpose

    400 nt l31 promoter region, l31 5'UTR with nt. 35-46 deleted, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Nucleotides 35-46 deleted from rpmE 5'UTR, O…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge