We narrowed to 31,702 results for: FER

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pAAV-EF1a-FLuc-WPRE-HGHpA

Plasmid

#87951

Purpose

Recombinant single-stranded AAV transfer vector expressing Firefly Luciferase under the EF1a promoter

Depositor

Mark Kay

Article

Paulk et al Mol Ther. 2017 Sep 25. pii: S1525-0016(1

Insert

Firefly Luciferase

Use

AAV

Promoter

EF1a

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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MLRV

Plasmid

#118069

Purpose

Multi-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reporters

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Inserts

TB:5xNF-κβ:RedF:bGHpA
TB:4xTGF-β:FLuc:bGHpA
TB:5xE-box:Renilla:bGHpA
TB:2xp53:NLuc:bGHpA
TB:6xAP-1:GrRenilla:bGHpA
CMV:ELuc:bGHpA

Use

Luciferase and Synthetic Biology; Assembly of gb2…

Expression

Mammalian

Promoter

2xp53::miniP, 4xTGF-B::miniP, 5xEbox::miniP, 5xNF…

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pKGmDUET-NgBR/hCIT

Plasmid

#203173

Purpose

Expression of human cis-prenyltransferase complex for purification

Depositor

William Sessa

Article

Grabinska et al J Biol Chem. 2017 Oct 20;292(42):17351-1

Inserts

NUS1 (NUS1 Human)
DHDDS (DHDDS Human)

Tags

Strep-Tag II-TEV and internally 6HIS tagged

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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mini-YFP-hDF-C2F-TM

Plasmid

#72600

Purpose

mini human dysferlin (from domain F to transmembrane ) tagged with YFP

Depositor

Renzhi Han

Article

Cheng et al Nat Med. 2014 Oct;20(10):1187-92. doi: 1

Insert

dysferlin (DYSF Human)

Tags

EYFP

Expression

Mammalian

Mutation

from domain F to transmembrane domain

Promoter

CMV

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only
pelB-MBP-BB0323-ss

Plasmid

#137067

Purpose

E. coli expression clone (T7lac promoter) for mature BB0323 (aa 20-377) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Insert

AAC66700.1

Tags

PelB signal peptide + His10 + maltose binding pro…

Expression

Bacterial

Mutation

mature BB0323: lacks the BB0323 signal peptide

Promoter

T7lac

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-camk2-Sthk-p2a-bpac(WT) minWPRE

Plasmid

#118274

Purpose

Expresses the SthK channel & bPAC(WT)-mCherry under a minimal CamKII promoter

Depositor

Dietmar Schmitz

Article

Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

Inserts

bPAC(WT)
SthK

Use

AAV

Tags

His tag, P2A, mCherry, and myc tag

Expression

Mammalian

Promoter

CamK II

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pOmnibac_ZZ_linker_ybbR_MAP7

Plasmid

#159718

Purpose

Expresses MAP7 in Sf9 cells with a ZZ affinity tag and a ybbR tag

Depositor

Ahmet Yildiz

Article

Ferro et al Science. 2022 Jan 21;375(6578):326-331.

Insert

MAP7 (Microtubule Associated Protein 7) (MAP7 Human)

Tags

ZZ affinity tag, ybbR tag

Expression

Insect

Available Since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-HALO-hLRRK2 (LIR1 mutant WEVL -> AEVA)

Plasmid

#229742

Purpose

Expresses Halo tagged human LRRK2 with LIR1 point mutations in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

LRRK2 (LRRK2 Human)

Tags

Halo

Expression

Mammalian

Mutation

LIR1 mutant WEVL -> AEVA

Promoter

EF1A

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-HALO-hLRRK2 (LIR2 mutant WTFI -> ATFA)

Plasmid

#229743

Purpose

Expresses Halo tagged human LRRK2 with LIR2 point mutations in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

LRRK2 (LRRK2 Human)

Tags

Halo

Expression

Mammalian

Mutation

LIR2 mutant WTFI -> ATFA

Promoter

EF1A

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-ACSL4-(-1899)

Plasmid

#202514

Purpose

Expressing luciferase driven by human ACSL4 promoter (bases -1 to -1899 with respect to exon1 start site)

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

ACSL4 promoter (partial) (ACSL4 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-ACSL4-(-706/-277)

Plasmid

#202511

Purpose

Expressing luciferase driven by human ACSL4 promoter (bases -277 to -706 with respect to exon1 start site)

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

ACSL4 promoter (partial) (ACSL4 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-ACSL4-(-254)

Plasmid

#202512

Purpose

Expressing luciferase driven by human ACSL4 promoter (bases -1 to -254 with respect to exon1 start site)

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

ACSL4 promoter (partial) (ACSL4 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-ACSL4-(-706)

Plasmid

#202513

Purpose

Expressing luciferase driven by human ACSL4 promoter (bases -1 to -706 with respect to exon1 start site)

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

ACSL4 promoter (partial) (ACSL4 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pU6-chiRNA_dCrkgRNA1

Plasmid

#131139

Purpose

Encodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

dCrk gRNA1 (Crk Fly)

Use

CRISPR

Expression

Insect

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-chiRNA_dCrkgRNA2

Plasmid

#131140

Purpose

Encodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

dCrk gRNA1 (Crk Fly)

Use

CRISPR

Expression

Insect

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pOmnibac_ZZ_linker_K963_GFP_SNAP

Plasmid

#159727

Purpose

Expresses kinesin-1 (amino acids 1-963) in Sf9 cells with a C-terminal GFP and SNAP tag

Depositor

Ahmet Yildiz

Article

Ferro et al Science. 2022 Jan 21;375(6578):326-331.

Insert

KIF5B (full length, 1-963 amino acids), K963 (KIF5B Human)

Tags

GFP and SNAP tags and ZZ affinity tag

Expression

Insect

Available Since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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CLYBL-TO-hNGN2-BSD-mApple

Plasmid

#124229

Purpose

Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.

Depositor

Michael Ward

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

NGN2 (NEUROG2 Human)

Expression

Mammalian

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti PGK FKBP12(F36V)-OGT (Neo)

Plasmid

#154294

Purpose

Lentiviral vector encoding FKBP12(F36V)-2xHA-OGT (Neomycin resistant) off PGK promoter

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Lentiviral

Tags

2x HA and FKBP12(F36V)

Expression

Mammalian

Promoter

PGK

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pF CAG luc IRES neo

Plasmid

#98294

Purpose

For constitutive expression of firefly luciferase from the CAG promoter together with an IRES-linked neo resistance gene

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Inserts

Firefly luciferase
IRES
Neomycin resistance gene (NeoR)

Use

Lentiviral and Luciferase

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pelB-MBP-HtrA-ss

Plasmid

#137066

Purpose

E. coli expression clone (T7lac promoter) for mature HtrA (aa 28-474) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Insert

AAC66500.2 (BB_0104 Borrelia burgdorferi B31)

Tags

PelB signal peptide + His10 + maltose binding pro…

Expression

Bacterial

Mutation

mature HtrA: lacks the HtrA signal peptide

Promoter

T7lac

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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