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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,794 results for: PORA

Showing: 1161 - 1180 of 1794 results

  4. RGB-S reporter

    Plasmid
    #207841
    Purpose
    A three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coli
    Depositor
    Christof Niemeyer
    Article
    Zoheir et al NPJ Biofilms Microbiomes. 2023 Aug 21;9(
    Inserts
    RpoH sensing construct
    SOS sensing construct
    RpoS sensing construct
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PosmY, PsulA, and grpE
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    207841-plasmid-map-sequence-id-426759

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  5. pAAV-CAG-JNKKTRrsGreenF-E-2A-ERKKTRrsFastLime-IRES-PKAKTRClover-2A-P38KTRDronpa

    Plasmid
    #205766
    Purpose
    Expression of JNK KTR rsGreenF-E, ERK KTR rsFastLime, PKA KTR Clover, and P38 KTR Dronpa in mamalian cells
    Depositor
    Edward Boyden
    Article
    Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.
    Insert
    JNK KTR rsGreenF-E-2A-ERK KTR rsFastLime-IRES-PKA KTR Clover-2A-P38 KTR Dronpa
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    205766-plasmid-map-sequence-id-459162

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  11. cyt-GafD-mTurboID-V5

    Plasmid
    #184640
    Purpose
    Expresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome tagging
    Depositor
    Charlie Fehl
    Article
    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021
    Insert
    cyt-GlycoID-V5
    Use
    Tags
    Nuclear export sequence and V5 tag
    Expression
    Mammalian
    Mutation
    GafD -> expressed aa22-178 ; BirA* -> expre…
    Promoter
    CMV
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184640-plasmid-map-sequence-id-366751

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  12. pSC-cUnaG-P2A-nUnaG-ST-P2A-mCh

    Plasmid
    #207634
    Purpose
    A plasmid encoding photocaged SpyCatcher (pSC)-cUnaG, nUnaG-SpyTag (ST), and mCherry separated by P2A cleaving sequences for mammalian expression.
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    photocaged SpyCatcher-cUnaG-P2A-nUnaG-SpyTag-P2A-mCh
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Amber stop codon at SC's critical lysine
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207634-plasmid-map-sequence-id-413133

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  14. nuc-GafD-mTurboID-HA

    Plasmid
    #184641
    Purpose
    Expresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome tagging
    Depositor
    Charlie Fehl
    Article
    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021
    Insert
    nuc-GlycoID-HA
    Use
    Tags
    3xHA tag and Nuclear localization sequence
    Expression
    Mammalian
    Mutation
    GafD -> expressed 22-178 ; BirA* -> express…
    Promoter
    CMV
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184641-plasmid-map-sequence-id-366747

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  16. pAAV-hSyn-GRAB_OTmut

    Plasmid
    #185388
    Purpose
    Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in neurons
    Depositor
    Yulong Li
    Article
    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Has Service
    AAV1 and AAV9
    Insert
    GPCR activation based oxytocin control sensor GRAB_OTmut
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    Sept. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185388-plasmid-map-sequence-id-372581

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  18. LUC01 (L/Sec/P)

    Plasmid
    #112144
    Purpose
    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity
    Depositor
    Paul Copeland
    Article
    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.
    Insert
    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Rat, Photinus pyralis)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Cysteine 258 mutated to selenocysteine (U)
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112144-plasmid-map-sequence-id-214867

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Showing: 1161 - 1180 of 1794 results