We narrowed to 159,237 results for: addgene

1. AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)_FS

   Plasmid

   #191527

   Purpose

   Donor for targeted integration of 3xFLAG-2xSTREP tagged S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus

   Depositor

   Yannick Doyon

   Insert

   hGEM(1-110)_mNG_hSLBP(18-108)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   mNeonGreen (mNG) + 3xFLAG_TwinStrep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. ChR2-YFP-NavII-III

   Plasmid

   #26057

   Depositor

   Matthew Grubb

   Article

   Grubb et al PLoS One. 2010 . 5(10):e13761.

   Insert

   NavII-III
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

3. CBX7

   Plasmid

   #25241

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CBX7_04 (CBX7 Human)
   

   Use

   Tags

   6x His and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 13, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

4. JMJD1CA-c089

   Plasmid

   #79644

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   JMJD1C (JMJD1C Human)
   

   Use

   Tags

   6x His and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. p-dCas9-p300-Hygro

   Plasmid

   #104411

   Purpose

   transient expression of dCas9-p300 fusion protein

   Depositor

   Stephan Beck

   Insert

   p300 (EP300 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJUNB.1.0-gDNA

   Plasmid

   #112409

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor JUNB

   Depositor

   Kevin White

   Insert

   JUNB (JUNB Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDB4279

   Plasmid

   #98698

   Purpose

   A control plasmid containing the intact bsdMX marker

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. ACE2 g2

   Plasmid

   #153012

   Purpose

   A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry

   Depositor

   Jason Sheltzer

   Insert

   ACE2 gRNA (ACE2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pINDUCER20-FLAG-hPAX3 (#735)

   Plasmid

   #78336

   Purpose

   Doxycycline-inducible lentiviral overexpression of human PAX3 ORF with a FLAG tag

   Depositor

   Matthew Alexander, Louis Kunkel

   Insert

   PAX3 (PAX3 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   TRE2

   Available since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

10. 6C1A (MBD2)

    Plasmid

    #119958

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    MBD2 (MBD2 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pNM3732

    Plasmid

    #153086

    Purpose

    mec-4p GFP-C1 positive control for RMCE integration

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    mec-4p GFP-C1 tbb-2 3'
    

    Use

    Rmce

    Tags

    Expression

    Worm

    Mutation

    Promoter

    not applicable

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. USP21 (3MTN)

    Plasmid

    #28112

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    USP21 (USP21 Human)
    

    Use

    Tags

    His tag with Thrombin cleavage

    Expression

    Bacterial

    Mutation

    catalytic domain only (amino acid residues 209-56…

    Promoter

    Available since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

13. ACE2 g3

    Plasmid

    #153013

    Purpose

    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry

    Depositor

    Jason Sheltzer

    Insert

    ACE2 (ACE2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCM130

    Plasmid

    #45828

    Depositor

    Mary Lidstrom

    Article

    Marx et al Microbiology. 2001 Aug;147(Pt 8):2065-75

    Type

    Empty backbone

    Use

    Low-background broad-host-range promoter-probe ve…

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG306-GPD-empty chr I

    Plasmid

    #41895

    Depositor

    Dan Gottschling

    Article

    Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    April 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. CBX4

    Plasmid

    #25237

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CBX4_02 (CBX4 Human)
    

    Use

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

17. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1

    Plasmid

    #118157

    Purpose

    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDS

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-P2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. FGD5

    Plasmid

    #28109

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    FGD5:HPC09X-H08:C212499 (FGD5 Human)
    

    Use

    Tags

    His-tag with TEV site

    Expression

    Bacterial

    Mutation

    contains amino acid residues 889-1304.

    Promoter

    Available since

    March 21, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pRL488

    Plasmid

    #70263

    Purpose

    designed to facilitate transcriptional fusions to luxAB, encoding luciferase, on a plasmid that can replicate in both Escherichia coli and strains of filamentous cyanobacteria.

    Depositor

    Peter Wolk

    Article

    Elhai et al EMBO J. 1990 Oct;9(10):3379-88.

    Type

    Empty backbone

    Use

    Luciferase; Cyanobacteria expression

    Tags

    luxAB

    Expression

    Mutation

    Promoter

    none

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIVT-myc-T58A-S62D

    Plasmid

    #138540

    Purpose

    For making myc-T58A-S62D RNA

    Depositor

    Carmen Williams

    Insert

    Myelocytomatosis oncogene T58A S62D (Myc Mouse)
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Threonine 58 changed to alanine and serine 62 cha…

    Promoter

    T7

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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