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Showing: 1161 - 1180 of 1215 results

  1. pEGFPC1-Cerulean3-Cerulean3-FLARE-D1ER

    Plasmid
    #123354
    Purpose
    Cyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator, targeted to endoplasmic reticulum lumen.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    cp-moxCerulean3-moxCerulean3-FLARE-D1ER
    Use
    Tags
    C-terminal ER retention signal (KDEL), cp-moxCeru…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    123354-plasmid-map-sequence-id-311156

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  3. pCAG-2dV-Camui (wt)

    Plasmid
    #220366
    Purpose
    Improved FLIM sensor for CaMK2-alpha activation (mammalian expression, cytosol)
    Depositor
    Ryohei Yasuda
    Article
    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-
    Insert
    mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(wt)-mEGFP(A206K) (Camk2a Synthetic, Rat)
    Use
    Tags
    mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    220366-plasmid-map-sequence-id-434512

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  8. CaV1.3e[8a,11,31b,Δ32,Δ42a]

    Plasmid
    #49332
    Purpose
    Repaired Addgene #26577 to agree with the rat ref sequence. Repairs are @: nt 6224 (GCC->GTC) [Ala->Val]; nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].
    Depositor
    Diane Lipscombe
    Article
    Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.
    Insert
    CACNA1D (Cacna1d Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains exons 8a, 11, and 31b.Exons 32 and 42a a…
    Promoter
    CMV
    Available since
    Feb. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    49332-plasmid-map-sequence-id-215242

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  12. pAAV-hSyn-DIO {hCAR}off-{GCaMP6f}on-W3SL

    Plasmid
    #111394
    Purpose
    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON GCaMP6f (ultrasensitive calcium sensor), and W3SL regulatory cassette (for maximize cloning capacity)
    Depositor
    Adam Kepecs
    Article
    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi
    Inserts
    hCAR (CXADR Human)
    GCaMP6f
    Use
    AAV
    Tags
    6xHis, Myc, T7, and Xpress
    Expression
    Mammalian
    Mutation
    Promoter
    hSynapsin
    Available since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    111394-plasmid-map-sequence-id-212394

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  15. Cav2.1 EA2, R57A, R59A pMT2

    Plasmid
    #206122
    Purpose
    expression of rabbit Cav2.1 calcium channel with a CaV2.1-P1217fs mutation causing a truncated protein, (EA2). R57A R59A mutations prevent dominant-negative suppression of Cav2.1 currents
    Depositor
    Annette Dolphin
    Article
    Dahimene et al Neurobiol Dis. 2016 Sep;93:243-56. doi:
    Insert
    cacna1a (Cacna1a Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Cav2.1-P1217fs, R57A, R59A
    Promoter
    Ad MLP/TPL/SV40
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    206122-plasmid-map-sequence-id-415911

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  16. GFP Cav2.2 human HAgHA-plus exon 18a - Short-Cterm - Arginine -in pcDNA3

    Plasmid
    #198915
    Purpose
    N-terminal GFP tagged, exofacial HAgHA tag in Domain II, contains exon 18a, Short form of alternatively spliced C-teminus, SNP rs2278973 variant Arginine, in pcDNA3 vector
    Depositor
    Annette Dolphin
    Article
    Dahimene et al Function (Oxf). 2023 Oct 19;5(1):zqad060
    Insert
    Cav2.2 (CACNA1B Human)
    Use
    Tags
    N-terminal GFP, internal double HA
    Expression
    Mammalian
    Mutation
    snp rs2278973 Arginine
    Promoter
    CMV
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    198915-plasmid-map-sequence-id-392113

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  17. GFP Cav2.2 human HAgHA -plus exon 18a - LONG-Cterm in pcDNA3

    Plasmid
    #198451
    Purpose
    N-terminal GFP tagged, exofacial HAgHA tag in Domain II, contains exon 18a- Long form of alternatively spliced C-teminus, in pcDNA3 vector
    Depositor
    Annette Dolphin
    Article
    Dahimene et al Function (Oxf). 2023 Oct 19;5(1):zqad060
    Insert
    Cav2.2 (CACNA1B Human)
    Use
    Tags
    N-terminal GFP, internal double HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    198451-plasmid-map-sequence-id-392120

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  18. pCAG-2dV-Camui (T305D/T306D)

    Plasmid
    #220368
    Purpose
    T305D/T306D phosphomimetic mutant of pCAG-2dV-Camui
    Depositor
    Ryohei Yasuda
    Article
    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-
    Insert
    mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T305D/T306D)-mEGFP(A206K) (Camk2a Synthetic, Rat)
    Use
    Tags
    mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)
    Expression
    Mammalian
    Mutation
    changed both Threonine 305 and 306 to aspartic ac…
    Promoter
    CAG
    Available since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    220368-plasmid-map-sequence-id-469102

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  19. pAAV-UBC-S1-GCaMP6f

    Plasmid
    #160722
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter GCaMP6f (calcium indicator), Xpress tag, and S1 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S1-GCaMP6f
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160722-plasmid-map-sequence-id-315521

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  20. pSYC-437

    Plasmid
    #163829
    Purpose
    Foxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.
    Depositor
    Seok-Yong Choi
    Article
    Zhang et al Nat Commun. 2020 Apr 20;11(1):1860. doi:
    Inserts
    Foxj1a Promoter
    GCaMP6s
    Use
    Multisite gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163829-plasmid-map-sequence-id-323196

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Showing: 1161 - 1180 of 1215 results