We narrowed to 29,405 results for: des.2

1. SARS-CoV-2 Spike-d18

   Plasmid

   #164583

   Purpose

   A mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.

   Depositor

   Jason Sheltzer

   Insert

   SARS-CoV-2 Spike (S SARS-CoV-2)
   

   Expression

   Mammalian

   Mutation

   Deleted amino acids 1256-1273

   Promoter

   EF1a

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1 RagD shRNA #2

   Plasmid

   #30322

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   RagD shRNA (RRAGD Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   June 2, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pSF11-wmiRFP670-2-T2A-HO1

   Plasmid

   #197250

   Purpose

   Expresses the protein of wmiRFP670-2-T2A-HO1 in neurons of C. elegans

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   wmiRFP670-2-T2A-HO1
   

   Expression

   Worm

   Available Since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-ef1a-DIO-eGCaMP(2+)

   Plasmid

   #201150

   Purpose

   AAV virus production for Cre recombinase dependent expression of eGCaMP(2+)

   Depositor

   Andre Berndt

   Article

   Wait et al Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi:

   Insert

   eGCaMP_2+
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Expression

   Mammalian

   Available Since

   June 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1 RagB shRNA #2

   Plasmid

   #26628

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   RagB shRNA (RRAGB Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pGEX6-2 SNAP-Syk tSH2

   Plasmid

   #113028

   Purpose

   for purification of Syk tSh2. Purified protein will have a snap tag that can be conjugated to fluorescent dyes.

   Depositor

   Ron Vale

   Article

   Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

   Insert

   SNAP-Syk tSH2
   

   Tags

   SNAP

   Expression

   Bacterial

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEBB HA BIR 2-3

   Plasmid

   #25691

   Depositor

   Colin Duckett

   Article

   Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

   Insert

   XIAP (XIAP Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   amino acid 124RDH-LEE350 (BIR 2&3)

   Available Since

   Aug. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pSUPER-retro-puro-shNup133-2

   Plasmid

   #87333

   Purpose

   To express shRNA against human Nup133

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   shRNA against human Nup133
   

   Use

   RNAi

   Expression

   Mammalian

   Promoter

   H1

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. psiCHECK-2-nMyc-m2_3'UTR

   Plasmid

   #31860

   Depositor

   Robert Blelloch

   Article

   Melton et al Nature. 2010 Feb 4;463(7281):621-6. Epub

   Insert

   nMyc mutant-1 3'UTR
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Second Let-7c Seed changed from TACCTC to TAACGC

   Promoter

   SV40

   Available Since

   Feb. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-H11r1-2-DD-Cas9

    Plasmid

    #85978

    Purpose

    Expresses DD-SpCas9 in mammalian cells and targets the H11 locus in human cells

    Depositor

    Michele Calos

    Article

    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

    Insert

    DD-SpCas9
    

    Use

    CRISPR

    Tags

    FKBP12 L106P DD

    Expression

    Mammalian

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. p8390 LentiCRISPRv2 Neo sgNT-2

    Plasmid

    #221650

    Purpose

    Expression of non-targeting control sgRNA

    Depositor

    Elizabeth White

    Article

    Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

    Insert

    non-targeting sgNT-2
    

    Use

    CRISPR and Lentiviral

    Available Since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGTag Hygro EGFP nLAP -2

    Plasmid

    #194322

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

    Insert

    Hygro-P2A-nLAP(EGFP)
    

    Use

    Bacterial cloning vector

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGTag Hygro BFP nLAP -2

    Plasmid

    #194325

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

    Insert

    Hygro-P2A-nLAP(BFP)
    

    Use

    Bacterial cloning vector

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGTag Hygro BFP nLAP +2

    Plasmid

    #194319

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

    Insert

    Hygro-P2A-nLAP(BFP)
    

    Use

    Bacterial cloning vector

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGTag Hygro EGFP nLAP +2

    Plasmid

    #194307

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

    Insert

    Hygro-P2A-nLAP(EGFP)
    

    Use

    Bacterial cloning vector

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVH230-2-Tier1-PmPGK1-YPet

    Plasmid

    #169521

    Purpose

    Tier-1 vector encoding PmPGK1-driven YPet expression (PmPGK1-YPet-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven yellow fluorescent protein Ypet
    

    Expression

    Mammalian

    Promoter

    PPGK

    Available Since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVH237-2-Tier1-PmPGK1-TtgA

    Plasmid

    #169584

    Purpose

    Tier-1 vector encoding PmPGK1-driven TtgA expression (PmPGK1-TtgA-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    Regulatory protein TtgR fused to a VP16 domain
    

    Expression

    Mammalian

    Promoter

    PPGK

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPD187 Tri-AND Gate, 2 repeats

    Plasmid

    #138940

    Purpose

    EYFP reporter vector for the Logic 37 AND 43 AND 158 2 repeats promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    Logic 37 AND 43 AND 158 2 repeats
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    Logic 37 AND 43 AND 158 2 repeats

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. psiCHECK-2-nMyc-m1_3'UTR

    Plasmid

    #31859

    Depositor

    Robert Blelloch

    Article

    Melton et al Nature. 2010 Feb 4;463(7281):621-6. Epub

    Insert

    nMyc mutant-1 3'UTR
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    first Let-7c Seed changed from TACCTC to TAACGC

    Promoter

    SV40

    Available Since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pBbS5a-Opto-Cre-Vvd-2

    Plasmid

    #160400

    Purpose

    Updated light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.

    Depositor

    Mary Dunlop

    Article

    Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

    Insert

    Opto-Cre-Vvd-2
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Bacterial

    Promoter

    lacUV5

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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