We narrowed to 7,122 results for: ef1a

1. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R_pp

   Plasmid

   #185061

   Purpose

   Ef1a driven PE2 with pegRNA for editing C201R mutation (including silent PAM mutation) in KCNQ2. See Addgene plasmid #184445

   Depositor

   Bobby Koeleman

   Insert

   U6:pegRNA:scaffold:PBS+RT template
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKLV2-EF1a-ArgSen (-)P2A (-)NLS

   Plasmid

   #184690

   Purpose

   Lentiviral transfer plasmid for expression of ArgSen (-)P2A (-)NLS

   Depositor

   Brian Van Tine

   Article

   Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

   Insert

   ArgSen (-)P2A (-)NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLV-EF1a-ArgSen (-)P2A (-)NLS

   Plasmid

   #184699

   Purpose

   Lentiviral transfer plasmid for expression of ArgSen (-)P2A (-)NLS

   Depositor

   Brian Van Tine

   Article

   Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

   Insert

   ArgSen (-)P2A (-)NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-EF1a-fDIO-tdTomato-WPRE

   Plasmid

   #187112

   Purpose

   FLP-dependent expression of tdTomato under EF1a promoter

   Depositor

   Yongling Zhu

   Article

   Xu et al Cell Rep. 2022 Jul 5;40(1):111036. doi:

   Insert

   tdTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLV-Hygro-EF1A-RG-Linker-eGFP

   Plasmid

   #176071

   Purpose

   EGFP fused to the C-terminus of a RG domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   RG
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLV-Hygro-EF1A-KR-Linker-eGFP

   Plasmid

   #176066

   Purpose

   EGFP fused to the C-terminus of a KR domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   KR
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LV_EF1a_RalA(G23V,full)-P2A-Hygro_Barcode

   Plasmid

   #170249

   Purpose

   Barcoded lentiviral vector to express RalA(G23V, full) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   RALA (RALA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   G23V

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLV-EF1a-KIF5AR280H-HA-IRES-Puro

   Plasmid

   #166953

   Purpose

   Lentiviral plasmid expressing HA-tagged KIF5A R280H protein with IRES-Puro from the EF1a promoter

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   KIF5A-R280H (Kif5a Mouse)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   KIF5A-R280H

   Promoter

   eF1a

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJM466 EF1a VP16-ZF43 (TUPV2)

   Plasmid

   #138730

   Purpose

   mMoClo TUPV, with EF1alpha promoter and VP16-ZFa in the MCS

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   VP16-ZF43
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hEF1alpha

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EF1a-3NLS-Klf4 TALE-GCN4

    Plasmid

    #120546

    Purpose

    Express 3xNLS-Klf4 TALE-GCN4 engineered to bind a site in the human KLF4 gene

    Depositor

    Yu Wang

    Article

    Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

    Insert

    NLS, Klf4 TALE, GCN4 (KLF4 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pOTTC1079 - pAAV TH gRNA A EF1a EGFP

    Plasmid

    #113159

    Purpose

    An AAV vector that expresses guide RNA targeting rat TH and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    gRNA for rat TH
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHAGE2-EF1alpha-HA-TFAP4(E135A/S139A)

    Plasmid

    #110159

    Purpose

    Lentiviral vector for expression of human TFAP4 (E135A/S139A) in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

    Insert

    TFAP4 (transcription factor AP-4) (TFAP4 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E135A/S139A

    Promoter

    CMV

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRRLNeo-pEF1a-p53ashL344A-mKate2-splitmVenusN

    Plasmid

    #69584

    Purpose

    Expresses mutated p53-L344A tagged with mKate2 and split N-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53-L344A (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - N term

    Expression

    Mammalian

    Mutation

    p53 L344A mutation that prevents tetramerization …

    Promoter

    EF1alpha

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRRLHygro-pEF1a-p53ashL344A-mKate2-splitmVenusC

    Plasmid

    #69585

    Purpose

    Expresses mutated p53-L344A tagged with mKate2 and split C-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53-L344A (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - C term

    Expression

    Mammalian

    Mutation

    p53 L344A mutation that prevents tetramerization …

    Promoter

    EF1alpha

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-EF1a-hChR2(H134R)-EYFP-WPRE

    Plasmid

    #20942

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    EF1alpha

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV-EF1A-DIO-Gephyrin.FingR-eGFP-CCR5TC

    Plasmid

    #126217

    Purpose

    Labeling of inhibitory synapses (green fluorescence) in cre-expressing neurons

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    Gephyrin.FingR-eGFP-CCR5TC
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLVX-EF1a-EGFP-VAMP5-IRES-Puromycin

    Plasmid

    #134662

    Purpose

    Expresses EGFP-tagged plasma membrane marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    vesicle-associated membrane protein 5 (VAMP5 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV-EF1A-DTX2-1-178-EGFP-Hygro

    Plasmid

    #238094

    Purpose

    Expression of structurally predicted WWE domain of DTX2 with EGFP on C terminal

    Depositor

    Robert Sobol

    Article

    Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

    Insert

    DTX2 (DTX2 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 1-178 only

    Promoter

    EF1alpha

    Available since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJM456 EF1alpha EBFP2-P2A-BlastR (TUPV7)

    Plasmid

    #139236

    Purpose

    mMoClo TUPV7, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    EBFP2-P2A-Blasticidin S Deaminase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

    Plasmid

    #118156

    Purpose

    Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-P2A-EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge