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pLV-HKGT4_short_mCherry2-NLS-PEST_Puro

Plasmid

#213774

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT4_short_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-HKGT4_mCherry2-NLS-PEST_Puro

Plasmid

#213773

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT4_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-HKGT3_short_mCherry2-NLS-PEST_Puro

Plasmid

#213772

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT3_short_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-HKGT2_mCherry2-NLS-PEST_Puro

Plasmid

#213769

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT2_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-HKGT2_short_mCherry2-NLS-PEST_Puro

Plasmid

#213770

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT2_short_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-HKGT1_mCherry2-NLS-PEST_Puro

Plasmid

#213767

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT1_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-HKGT1_short_mCherry2-NLS-PEST_Puro

Plasmid

#213768

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT1_short_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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eA3Ai-Cas9n-UGI-NLS

Plasmid

#207165

Purpose

Express A3A with an N57G point mutation and an intron in a BE3-based base editor

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3A (APOBEC3A Human)

Tags

Cas9-UGI--NLS

Expression

Mammalian

Mutation

N57G

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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PET-HypaSpCas9-NLS-6xHis

Plasmid

#207384

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HypaSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N692A, M694A, Q695A, H698A

Promoter

T7

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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PET-HeFSpCas9-NLS-6xHis

Plasmid

#207391

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HeFSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

Promoter

T7

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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PET-evoSpCas9-NLS-6xHis

Plasmid

#207389

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

evoSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

M495V, Y515N, K526E, R661Q

Promoter

T7

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR1a-jAspSnFR3-mRuby3-NLS

Plasmid

#203489

Purpose

Nuclear directed jAspSnFR3-mRuby3 in Gateway cloning entry vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3-mRuby3-NLS

Use

Gateway Cloning

Tags

Nuclear localization signal and mRuby3

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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iB22 PB-zim3-mycNLS-mApple

Plasmid

#204718

Purpose

Constitutive CRISPRi by Zim3-dCas9

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Zim3-dCas9

Expression

Mammalian

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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MCS-T2a-GFPnls-ER-Cre-ER

Plasmid

#200104

Purpose

Vector For Twist

Depositor

Prayag Murawala

Type

Empty backbone

Use

Unspecified

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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Twist3cterm-GFPnls-T2A-ER-Cre-ER

Plasmid

#200105

Depositor

Prayag Murawala

Insert

Twist3:cTer-KI-eGFPnls-T2A-ER-Cre-ER

Use

Unspecified

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT IAA17-6FLAG (no NLS)

Plasmid

#193322

Purpose

C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration and does not have NLS tags..

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

IAA17

Tags

6FLAG

Expression

Yeast

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-2X(CMV-eBFP2-3XNLS-pA)

Plasmid

#195867

Purpose

to express nuclear localized eBFP2

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

eBFP2

Use

AAV

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV2-UbC-3xNLS-mScarlet-I

Plasmid

#191099

Purpose

To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

Depositor

Murray Blackmore, Pantelis Tsoulfas

Article

Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

Insert

3xNLS-mScarlet-I

Use

AAV and Synthetic Biology

Tags

Three repeats of the nuclear localizzation seque…

Expression

Mammalian

Mutation

optimized to human codon usage

Promoter

UbC

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-3x-NLS-mScarlet-AMPKα2

Plasmid

#192452

Purpose

mScarlet-tagged alpha2 isoform of AMPK catalytic subunit targeted to the nucleus.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

3x-NLS-mScarlet-AMPKalpha2 (Prkaa2 Rat)

Tags

3x Nuclear localization signal (NLS) and mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-ArgSen (-)NLS

Plasmid

#184689

Purpose

Lentiviral transfer plasmid for expression of ArgSen (-)NLS

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

ArgSen (-)NLS

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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