We narrowed to 6,084 results for: nls

1. P2A-NLS-tdtomato-EPG backbone

   Plasmid

   #215548

   Purpose

   An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of puromycin-EGFP

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. phage UbiC NLS HA stdMCP stdHalo

   Plasmid

   #104999

   Purpose

   Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labeling

   Depositor

   Jeffrey Chao

   Article

   Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

   Insert

   stdMCP-stdHalo
   

   Use

   Lentiviral

   Tags

   HA tag, NLS, Two synonymous copies of the Halo-ta…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   UbiC

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-NLS-iPASnFR-L-mScarlet3-3xFLAG

   Plasmid

   #225246

   Purpose

   Mammalian expression of iPASnFR-L in the nucleus

   Depositor

   Loren Looger

   Article

   Tamura et al bioRxiv 2024.08.21.609037

   Insert

   iPASnFR-L
   

   Use

   Tags

   3xFLAG and mScarlet3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-2xflox-BRX-eGFP-NLS

   Plasmid

   #217535

   Purpose

   AAV vector to drive the expression of eGFP in genetically defined cell populations under the control of the 4xBRE regulatory element of SMAD1 transcription factor and miniXon splicing casette in vivo

   Depositor

   Peter Scheiffele

   Article

   Okur et al bioRxiv 2023

   Insert

   4X BRE reporter and miniXon casette (Smad1 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   BMP response element (BRE)

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. phage-ubc-nls-ha-tdPCP-gfp

   Plasmid

   #40650

   Depositor

   Robert Singer

   Article

   Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

   Insert

   tdPCP-gfp
   

   Use

   Lentiviral

   Tags

   EGFP, HA, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   human ubiquitin C (UBC)

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-EFS-PCP-3xSNAP-NLS

   Plasmid

   #236388

   Purpose

   Overexpression of PCP-3xSNAP in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

   Insert

   PCP-3xSNAP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP

   Plasmid

   #31484

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   EF1s HA NLS Sce(opt) T2A IFP1.4
   

   Use

   Lentiviral

   Tags

   HA and NLS

   Expression

   Mutation

   "Opt" indicates the I-SceI coding seque…

   Promoter

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. phage-ubc-nls-ha-tdMCP-gfp

   Plasmid

   #40649

   Depositor

   Robert Singer

   Article

   Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

   Insert

   tdMCP-gfp
   

   Use

   Lentiviral

   Tags

   EGFP, HA, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   human ubiquitin C (UBC)

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV 4xSTAR-mNeonGreen-NLS-blast

   Plasmid

   #136258

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

   Insert

   stem cell Ascl2 reporter, 4 repeats
   

   Use

   Lentiviral

   Tags

   mNeonGreen-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. His-CL7-2NLS-SpyCas9(dS)-A22p3star

    Plasmid

    #222970

    Purpose

    Bacterial expression of A22p-fused SpyCas9 construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

    Insert

    His-CL7-2NLS-SpyCas9(dS)-A22p3
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. NLS-PyronicSF/pCMV-myc-nuc

    Plasmid

    #124814

    Purpose

    Highly resposive GFP-based pyruvate nanosensor with a nuclear localization signal.

    Depositor

    Luis Felipe Barros

    Article

    Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

    Insert

    PyronicSF
    

    Use

    Tags

    Nuclear Localization Signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-NES-DEVD-mCardinal-NLS

    Plasmid

    #164052

    Purpose

    Fluorescent reporter for caspase-3

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    NES-mCardinal-NLS
    

    Use

    Tags

    kozak sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promotor

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CAG-SV40NLSf-mScarletP2A-hLuc

    Plasmid

    #218793

    Purpose

    AAV reporter genome plasmid

    Depositor

    Ben Deverman

    Article

    Huang et al Science. 2024 Jun 14;384(6701):1220-1227

    Insert

    NLSf-mScarletP2A-hLuc
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV Marina-T2A-nls-mCherry

    Plasmid

    #74216

    Purpose

    genetically-encoded fluorescent voltage indicator

    Depositor

    Vincent Pieribone

    Article

    Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

    Insert

    fluorescent protein voltage sensor
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Ci-VSP contains R217Q mutation; super ecliptic pH…

    Promoter

    CMV

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS

    Plasmid

    #199252

    Purpose

    Expression construct encoding the high specificity eSpCas9(1.1)-D10A nickase

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Insert

    High specificity eSpCas9(1.1)-D10A nickase
    

    Use

    CRISPR

    Tags

    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A K848A K1003A R1060A

    Promoter

    CAG promoter

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PET-HiFi SpCas9-NLS-6xHis

    Plasmid

    #207376

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HiFi SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R691A

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. NLS-mCherry-NES reporter (pDN160)

    Plasmid

    #72660

    Purpose

    NLS-mCherry-NES 17 reporter for light-inducible nuclear export block with pDN157 and pDN158

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

    Insert

    NLS-mCherry-NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLR5-CAG-hMCP-mScarlet-I-NLS

    Plasmid

    #174878

    Purpose

    PiggyBac transposon vector for expression of MCP-mScarlet-I-NLS

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    MCP-mScarlet-I-NLS
    

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmCherry-C1 actin-3XNLS P2A mCherry

    Plasmid

    #58475

    Purpose

    Expresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

    Inserts

    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. phage UbiC NLS HA stdPCP stdGFP

    Plasmid

    #104099

    Purpose

    Lentiviral vector expressing synonymous tandem PP7 coat protein fused to synonymous tandem GFP. Used to label reporter mRNAs.

    Depositor

    Jeffrey Chao

    Article

    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

    Insert

    stdPCP-stdGFP
    

    Use

    Lentiviral

    Tags

    N-terminal NLS, Two synonymous copies of GFP (C-t…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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