We narrowed to 12,205 results for: 112

1. P521_VSX2_p2A-h2b_mRuby3

   Plasmid

   #239104

   Purpose

   Used in CRISPR gene editing to Insert a p2A-h2b_mRuby3 sequence before the stop codon of the endogenous human VSX2 gene

   Depositor

   Karl Wahlin

   Article

   Jones et al Cells. 2022 Oct 28;11(21):3412. doi: 10.

   Insert

   VSX2 (VSX2 Human)
   

   Use

   CRISPR

   Tags

   -p2A-h2b-mRuby3

   Expression

   Mammalian

   Promoter

   Endogenous VSX2 promoter

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. SmoM2 (W535L)-pcw107-V5

   Plasmid

   #64628

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   SMO (SMO Human)
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   W535L

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLX304-DCK*-GW-IRES-GFP

   Plasmid

   #176289

   Purpose

   Gateway vector for use in generating DCK*-POI fusion reporter

   Depositor

   William Kaelin

   Article

   Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

   Insert

   Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   S74E R104M D133A

   Promoter

   CMV

   Available Since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. MEK1 (S218D,S222D)-pcw107

   Plasmid

   #64604

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Use

   Lentiviral

   Mutation

   S218D, S222D

   Promoter

   PGK

   Available Since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-sgAMPKa2-Cas9-GFP

   Plasmid

   #208050

   Purpose

   Lentiviral vector expressing Cas9 and an sgRNA targeting AMPKa2

   Depositor

   Naama Kanarek

   Article

   Maynard et al Sci Adv. 2024 Feb 2;10(5):eadj9479. doi:

   Insert

   sgPRKAA2 (PRKAA2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. beta-catenin (S33Y)-pcw107

   Plasmid

   #64615

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   CTNNB1 (CTNNB1 Human)
   

   Use

   Lentiviral

   Mutation

   S33Y

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZ M2rtTA_CAGG TetON-Sox10 with GFP

   Plasmid

   #115241

   Purpose

   RMCE donor vector for inducible expression of SOX10 and GFP and constitutive expression of m2rtTA (generated from plasmid #112668)

   Depositor

   Catherine Verfaillie

   Article

   Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

   Inserts

   SOX10 (SOX10 Human)
   eGFP
   

   Tags

   GFP (separate polypeptide)

   Expression

   Mammalian

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-ABE8e-SpCas9-NRCH-P2A-EGFP (RMD57)

   Plasmid

   #197504

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-ABE8e-NRCH-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA and nSpCas9-NRCH(D10A/I32…

   Promoter

   CMV and T7

   Available Since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-dnMCAK

   Plasmid

   #208650

   Purpose

   Mammalian expression plasmid expressing eGFP-dnMCAK driven by CMV promoter

   Depositor

   Samuel Bakhoum

   Article

   Li et al Nature. 2023 Aug;620(7976):1080-1088. do

   Insert

   Kif2c (Kif2c Cricetulus griseus (Chinese hamster))
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   S92A , S106A , S108A, S112A , S186A, H530A, R534A…

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. RRL.sin.cPPT.SFFV/Ace2.IRES-puro.WPRE (MT126)

    Plasmid

    #145839

    Purpose

    Lentiviral vector genome to ectopically express human Ace2

    Depositor

    Caroline Goujon

    Article

    Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

    Insert

    human Ace2 (ACE2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. BCL-XL-pcw107-V5

    Plasmid

    #64631

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    BCL2L1 (transcript variant 1) (BCL2L1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

12. RRL.sin.cPPT.SFFV/TMPRSS2 (variant 1).IRES-neo.WPRE (MT130)

    Plasmid

    #145843

    Purpose

    Lentiviral vector genome to ectopically express human TMPRSS2 variant 1

    Depositor

    Caroline Goujon

    Article

    Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

    Insert

    human TMPRSS2 variant 1 (TMPRSS2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    G8V

    Promoter

    SFFV

    Available Since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX304-DCK*-IRES-GFP

    Plasmid

    #176297

    Purpose

    Expresses DCK* and GFP from the same transcript

    Depositor

    William Kaelin

    Article

    Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

    Insert

    Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    S74E R104M D133A

    Promoter

    CMV

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC0053-CMV-dPspCas13b-GS-ADAR2DD(E488Q)-delta-984-1090

    Plasmid

    #103869

    Purpose

    dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Inserts

    PspCas13b
    huADAR2DD (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    GS and HIV NES

    Expression

    Mammalian

    Mutation

    Changed histidne 133 to alanine and histidine 105…

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMIH-mRuby2-BAX

    Plasmid

    #111628

    Purpose

    Fluorescent fusion protein used to visualise mouse BAX, with hygromycin selection

    Depositor

    Benjamin Kile

    Article

    McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

    Insert

    Bax (Bax Mouse)
    

    Use

    Retroviral

    Tags

    mRuby2

    Expression

    Mammalian

    Promoter

    MSCV

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. TDP-REGv2(mScarlet-FLAG)#11 in pTwist-CMV backbone

    Plasmid

    #216155

    Purpose

    Expresses mScarlet in cells with TDP-43 loss-of-function. Very bright and decent dynamic range (>10-fold), slightly leaky. It uses a single intron. [Code 'B12']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    mScarlet with cryptic splice site and C-terminal FLAG tag
    

    Expression

    Mammalian

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTetOne-FLAG-human cGASdeltaN-HA

    Plasmid

    #186899

    Purpose

    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Human cGAS truncation mutant (160-522 a.a.) with N-terminal FLAG and C-terminal HA tag (Adamts1 Human, Synthetic)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Mutation

    N-terminal truncation

    Promoter

    TRE3G

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLX304-DCK*-IRES-Td

    Plasmid

    #176298

    Purpose

    Expresses DCK* and TdTomato from the same transcript

    Depositor

    William Kaelin

    Article

    Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

    Insert

    Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    S74E R104M D133A

    Promoter

    CMV

    Available Since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-Neo-CMV-MCL1

    Plasmid

    #202668

    Purpose

    MCL1 overexpression vector with G418 resistance

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    MCL1 apoptosis regulator, BCL2 family member (MCL1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPaGE Pyl TAG FliC T248TAG

    Plasmid

    #160089

    Purpose

    Encodes for MmPyl tRNA synthetase/tRNA pair and for flagellin protein (FliC) with TAG mutation at site 248, for unnatural amino acid incorporation into the flagellin protein in Pseudomonas aeruginosa.

    Depositor

    Lital Alfonta

    Article

    Ozer et al Sci Adv. 2021 Jun 11;7(24). pii: 7/24/ea

    Inserts

    Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
    Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
    Flagellin
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Changed Threonine 248 to TAG stop-codon.

    Promoter

    Pseudomonas aeruginosa Leu-tRNA native promoter a…

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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