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PHY98-LMNA 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

Plasmid

#164046

Purpose

CRISPR donor plasmid to insert TriTag (mTagBFP) into the N-terminus of human LMNA gene

Depositor

Baohui Chen

Article

Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

Insert

mTagBFG-TriTag

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp10-HF

Plasmid

#157708

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp10-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-FH-Nsp10

Plasmid

#157707

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp10 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-FH-Nsp9

Plasmid

#157704

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp9 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-shEsr2

Plasmid

#120722

Purpose

Lentiviral vector that expresses GFP and an shRNA targeting Esr2 (in pLL3.7).

Depositor

Amy Lasek

Article

Vandegrift et al J Neurosci. 2020 Jul 1;40(27):5196-5207.

Insert

Esr2 (Esr2 Mouse)

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mammalian

Promoter

mouse U6

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF8-2xCSTREP

Plasmid

#136172

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF8

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF8

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pPEE46

Plasmid

#139425

Purpose

Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mRuby3 ORF and a flexible linker at the 5' end.

Depositor

James Fraser

Article

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

mRuby3

Expression

Yeast

Promoter

TEF1

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF74-2xNSTREP

Plasmid

#136233

Purpose

Expresses N-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF74

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF74

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-K8.1-2xCSTREP

Plasmid

#136157

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K8.1

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K8.1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-PB-PGK-tetR-3xFLAG-HA-2A-Puro

Plasmid

#129537

Purpose

PiggyBac transposon carrying tetR-3xFLAG-HA-2A-Puro under the PGK promoter.

Depositor

Hideyuki Okano

Article

Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

Insert

tetR-3xFlag-HA-2A-Puro

Use

Synthetic Biology

Promoter

hPGK promoter

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pWZL-Stat5a1*6

Plasmid

#130669

Purpose

Retroviral vector for the expression of constitutive Stat5a (hygromycin resistance)

Depositor

Gerardo Ferbeyre

Article

Mallette et al J Biol Chem. 2007 Nov 30;282(48):34938-4

Insert

Stat5a (Stat5a Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

changed Histidine 298 to Arginine and Serine 710 …

Promoter

LTR

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pLC-CUL4A WT-P2A-Puro

Plasmid

#124304

Purpose

Lentiviral vector for expression of wild-type CUL4A-P2A-Puro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

CUL4A (CUL4A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCALNL-mutKir2.1

Plasmid

#125579

Purpose

Cre-dependent expression of a non-conducting mutant of Kir2.1 (FLAG-tagged) in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

Kir2.1 (Kcnj2 Mouse)

Use

Cre/Lox

Tags

FLAG

Expression

Mammalian

Mutation

A non-conducting mutant (GYG -> AAA).

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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JC101: pMVP/Ad/PK-DEST

Plasmid

#121845

Purpose

pMVP destination vector, empty pK fiber-modified adenovirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Adenoviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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PCRII apoEb

Plasmid

#121901

Purpose

Plasmid for making zebrafish apoEb in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

Apoeb (apoeb Zebrafish)

Use

Unspecified

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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lentiGuide-RRBP1-1

Plasmid

#92156

Purpose

CRISPR guide RNA targeting human RRBP1

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

RRBP1 sgRNA-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pRSV-p65S276A

Plasmid

#106455

Purpose

NF-kB p65, kinase site 276 mutated

Depositor

Georg Weber

Article

He et al Eur J Biochem. 2003 May . 270(10):2174-8

Insert

NF-kB p65, kinase site 276 mutated (RELA Human)

Use

Lentiviral

Expression

Mammalian

Mutation

kinase site inactivated

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shLacZ-1735

Plasmid

#115454

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shLacZ-1735

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pSum-LacZ-gfp

Plasmid

#109285

Purpose

Expresses gfp in mycobacteria

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

GFP

Expression

Bacterial

Mutation

GFP modified for expression in prokaryotes; inclu…

Promoter

LacZalpha

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEB1-mTurquoise2

Plasmid

#103974

Purpose

Plasmid encoding mTurquoise2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mTurquoise2

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, Y66W, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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