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pGEX-2TK cyclin T1 (1-551) (P#422)

Plasmid

#14609

Depositor

Matija Peterlin

Article

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (1-551) (CCNT1 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-551

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pAG69 G6PD 3'UTR mut

Plasmid

#12053

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

G6PD 3'UTR mut (G6PD Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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GST-CHMP4C (216-233) (pGEX2T(TEV))

Plasmid

#80635

Purpose

Residues 216-233 of CHMP4C; Bacterial Expression Vector; TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-202

Depositor

Wesley Sundquist

Article

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

CHMP4C (CHMP4C Human)

Tags

GST

Expression

Bacterial

Mutation

Residues 216-233

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pGST-mBCL10

Plasmid

#33316

Depositor

Vanessa Redecke

Article

Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

Insert

Bcl10 (Bcl10 Mouse)

Tags

GST

Expression

Bacterial

Available Since

Dec. 21, 2011

Availability

Academic Institutions and Nonprofits only
pgex6p-1mciap1mut

Plasmid

#11484

Depositor

Jon Ashwell

Article

Conze et al Mol Cell Biol. 2005 Apr . 25(8):3348-56.

Insert

mciap1mut (Birc2 Mouse)

Tags

GST

Expression

Bacterial

Mutation

582 to alanine

Available Since

March 8, 2006

Availability

Academic Institutions and Nonprofits only
MBP_TS-B1AR_Trx_PreSc_NdeI_AgeI

Plasmid

#247768

Purpose

This plasmid is a modified version of pRG/III-hs-MBP (Addgene plasmid #121676), designed for the expression of a functional β1-adrenergic receptor (β1AR) in E. coli.

Depositor

Stephan Grzesiek

Article

Abiko et al J Biomol NMR. 2021 Jan;75(1):25-38. doi:

Insert

Thermostable Turkey B1-adrenergic receptor (TS-B1AR)

Tags

MBP–(GS)5–HRV 3C site–(GS)5–NdeI site_ B1AR_AgeI …

Expression

Bacterial

Promoter

lac promoter

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-HALO-hLRRK2

Plasmid

#229738

Purpose

Expresses Halo tagged human LRRK2 in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

LRRK2 (LRRK2 Human)

Tags

Halo

Expression

Mammalian

Promoter

EF1A

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin K161N

Plasmid

#227365

Purpose

For bacterial expression of parkin with a mutated pUbl/pUb binding site on RING0.

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin K161N (PRKN Human)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin delta 101-109

Plasmid

#227364

Purpose

For bacterial expression of parkin with a mutated ACT.

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin delta 101-109 (PRKN Human)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Resi…

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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PABPC1_544-626_pGEX6P1

Plasmid

#209790

Purpose

Bacterial expression of PABPC1 Mlle domain

Depositor

Kalle Gehring

Article

Kozlov et al J Biol Chem. 2010 Apr 30;285(18):13599-6

Insert

Mlle domain of PABPC1 (PABPC1 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 544-626 only

Promoter

tac

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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rUBR5_2383-2442_pGEX6P1

Plasmid

#209791

Purpose

Bacterial expression of UBR5 Mlle domain

Depositor

Kalle Gehring

Article

Lim et al J Biol Chem. 2006 May 19;281(20):14376-8

Insert

Mlle domain of UBR5 (Ubr5 Rat)

Tags

GST

Expression

Bacterial

Mutation

aa 2383-2442 only

Promoter

tac

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-6xHis-TEV-ORF9c

Plasmid

#201023

Purpose

Bacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

open reading frame 9c

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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p21StBD

Plasmid

#190269

Purpose

Constitutively expresses a protein of interest fused to a GAL4 DNA-binding domain. Targeted integration to genomic locus YPRC-tau 3, Chromosome XVI.

Depositor

Assaf Mosquna

Article

Zimran et al ACS Synth Biol. 2022 Aug 19;11(8):2880-2

Type

Empty backbone

Use

Synthetic Biology; Yeast genome-integrating vector

Tags

GAL4-BD

Promoter

TDH3

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pDK366

Plasmid

#134196

Purpose

Retroviral plasmid with SKAP insert

Depositor

Iain Cheeseman

Article

Kern et al J Cell Biol. 2016 May 9;213(3):315-28. d

Insert

5xRD SKAP Alternate Start- LAP (with G5 hardeneded) (KNSTRN Human)

Use

Retroviral

Tags

LAP (GFP)

Expression

Mammalian

Mutation

5xRD, Alternate Start, G5 hardeneded

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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GST-CHMP4A (205-222) (pGEX2T(TEV))

Plasmid

#80633

Purpose

Residues 205-222 of CHMP4A, Bacterial Expression Vector; TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-60

Depositor

Wesley Sundquist

Article

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

CHMP4A (CHMP4A Human)

Tags

GST

Expression

Bacterial

Mutation

Residues 205-222;

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 Erg (1-208)

Plasmid

#66990

Purpose

Bacterial expression plasmid for mouse Erg (1-208) with GST

Depositor

Christopher Vakoc

Article

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

Erg (1-208) (Erg Mouse)

Tags

GST fusion

Expression

Bacterial

Mutation

Amino acids from 1-208

Promoter

TAC

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only
pLNCX2-mCherry-CHMP4B

Plasmid

#116923

Purpose

expresses mCherry-CHMP4B in mammalian cells

Depositor

Sanford Simon

Article

Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

Insert

CHMP4B (CHMP4B Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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Tol2-elavl3-H2B-GCaMP6s

Plasmid

#59530

Purpose

Expressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

Depositor

Misha Ahrens

Article

Freeman et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

Inserts

elavl3 promoter (elavl3 Zebrafish)
H2B
GCaMP6s

Use

Transposable element

Available Since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Ndi-1/myc-His

Plasmid

#127503

Purpose

Expresses myc-tagged yeast Ndi-1 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

Ndi-1 (NDI1 Budding Yeast)

Tags

myc-His

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX VAMP7 (1-188)

Plasmid

#42315

Depositor

Thierry Galli

Article

Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

Insert

VAMP7 Cytoplasmic domain (1-181) (VAMP7 Human)

Tags

GST

Expression

Bacterial

Mutation

Δ181-220

Promoter

Ptac

Available Since

March 12, 2013

Availability

Academic Institutions and Nonprofits only