We narrowed to 21,719 results for: ESC;
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Plasmid#103607PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-519a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-519a-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-509-5p
Plasmid#103591PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-509-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-509-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-135a-5p
Plasmid#103227PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-135a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-135a-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-1
Plasmid#103160PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-1 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-1 target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-mCherry-CHMP2B-siRNAres
Plasmid#115333Purposeexpresses siRNA-resistant mCherry-CHMP2B in mammalian cellsDepositorInsertCHMP2B (CHMP2B Human)
UseRetroviralTagsmCherryExpressionMammalianMutationmutated to be resistant to siRNA knockdownPromoterCMVAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#84
Plasmid#110878PurposeExpresses the C. elegans acy--1 P260S gain-of-function cDNA in ventral cord cholinergic motor neuronsDepositorInsertsunc-17beta promoter
acy-1 P260S gain-of-function cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationChanged Proline to Serine at amino acid 260PromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#81
Plasmid#110876PurposeExpresses the C. elegans acy-1 P260S gain-of-function cDNA in body wall muscleDepositorInsertsmyo-3 promoter
acy-1 P260S gain-of-function cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationChanged Proline to Serine at amino acid 260PromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#83
Plasmid#110877PurposeExpresses the C. elegans acy--1 P260S gain-of-function cDNA pan-neuronallyDepositorInsertsrab-3 promoter
acy-1 P260S gain-of-function cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationChanged Proline to Serine at amino acid 260PromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO h-Stx3S-2xMyc/His
Plasmid#99744PurposeExpresses human Stx3S with a C-term myc-myc-his tag in mammalian cells.DepositorInsertStx3S (STX3 Human)
UseTagsmyc-myc-hisExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMGF130
Plasmid#96971PurposeS. pombe lem2 knockout cassette with hygro resistance geneDepositorInsertlem2 (lem2 Fission Yeast)
UseTagsExpressionBacterialMutationwhole ORF deletion and replaced with hygro resist…PromoterN/AAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMGF169
Plasmid#96996Purposeish1 mCherry insertion cassette with G418 resistance geneDepositorInsertish1 (ish1 Fission Yeast)
UseTagsGST and mCherryExpressionBacterialMutationish1 C-terminal domain fused with mCherry + G418 …PromoterN/AAvailable sinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (205-222) (pGEX2T(TEV))
Plasmid#80633PurposeResidues 205-222 of CHMP4A, Bacterial Expression Vector; TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-60DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationResidues 205-222;PromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGB3omega1R Tnos:NptII:Pnos-SF (GB1181)
Plasmid#75471PurposeTranscriptional unit for kanamycin resistance geneDepositorInsertNptII
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAgrobacterium tumefaciens Nopaline synthase promo…Available sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEST-Pl-tetO1-tetR-linker-deGFP-T500 (109p)
Plasmid#70195PurposePost cloned 4-piece from Isothermal/Golden Gate assembly (GGA), using sequences from 2 (PltetO1-UTR1), 3 (tetR+linker from primer extension), 21 (linker from primer extension + deGFP), and 105 (vectorDepositorInserttetR-deGFP fusion
UseTagsExpressionMutationPromoterAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-mOC-STAMP (N162D)-GFP
Plasmid#73580PurposeLentivirus expression of mOC-STAMP with N162D mutationDepositorInsertOC-STAMP (Ocstamp Mouse)
UseLentiviralTagsGFPExpressionMutationchanged asparagin (N) 162 to Aspartic Acid (D)PromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMAL-PIAL2M-wt
Plasmid#67909PurposeInducible expression of PIAL2 in E.coliDepositorInsertPIAL2 (AT5G41580 Mustard Weed)
UseTagsMBPExpressionBacterialMutationE281-A496PromoterP-lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pONL-C1_fibrillarin
Plasmid#65710PurposeExpresses ONL-fibrillarin in mammalian cellsDepositorInsertfibrillarin (FBL Human)
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYNL-C1_fibrillarin
Plasmid#65708PurposeExpresses YNL-fibrillarin in mammalian cellsDepositorInsertfibrillarin (FBL Human)
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTYB-pLAC-I68S/I73S
Plasmid#48728PurposeBacterial expression of Lacritin Proteins with Mutation at protein 68 and 73 from Isoleucine to SerineDepositorInsertLacritin (LACRT Human)
UseTagsInteinExpressionBacterialMutationIsoleucine 68 and 73 to SerinePromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTYB-pLAC-L108S
Plasmid#48725PurposeBacterial expression of Lacritin Protein with Mutation at protein 108 from Leucine to SerineDepositorInsertLacritin (LACRT Human)
UseTagsInteinExpressionBacterialMutationLeucine 108 to SerinePromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only