We narrowed to 147,462 results for: ins;

1. PT7lacO-His6-IF1

   Plasmid

   #224366

   Purpose

   Express IF1 protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   IF1 (infA E. coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HA GST PreScission p70 S6K1

   Plasmid

   #15511

   Depositor

   David Sabatini

   Article

   Sancak et al Mol Cell. 2007 Mar 23. 25(6):903-15.

   Insert

   p70 S6K1 (Rps6kb1 Rat)
   

   Use

   Tags

   GST and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 1, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pET-2(KW)2-mCherry

   Plasmid

   #112738

   Purpose

   Express 2(KW)2-mCherry in E. coli

   Depositor

   Kyubong Jo

   Article

   Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

   Insert

   2(KW)2-mCherry
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKeratin-miRFP720-N1

   Plasmid

   #197236

   Purpose

   Expresses the fusion protein of Keratin-miRFP720 in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   Keratin-miRFP720
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. His_CL7_2NLS_iCas12a-4NLS

   Plasmid

   #222971

   Purpose

   Bacterial expression of NLS-rich iCas12a construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

   Insert

   His_CL7_2NLS_iCas12a-4NLS
   

   Use

   CRISPR

   Tags

   CL7 and His

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SRa-HA-SUMO2 (Sentrin 3)

   Plasmid

   #17360

   Purpose

   Mammalian expression of human SUMO2 with HA tag

   Depositor

   Edward Yeh

   Article

   Kamitani et al J Biol Chem. 1998 Feb 6. 273(6):3117-20.

   Insert

   SUMO2 (SUMO2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PS-CFP2-Farnesyl-5

   Plasmid

   #57227

   Purpose

   Localization: Membrane, Excitation: 400 / 490, Emission: 468 / 511

   Depositor

   Michael Davidson

   Insert

   Farnesyl (HRAS Human)
   

   Use

   Tags

   PS-CFP2

   Expression

   Mammalian

   Mutation

   encodes final 20 aa of NM_001130442.1

   Promoter

   CMV

   Available Since

   April 1, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pJK226

   Plasmid

   #71125

   Purpose

   Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

   Depositor

   T. J. Kappock

   Article

   Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

   Insert

   citrate synthase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL0_24 [pBBR1-UP ORI]

   Plasmid

   #198937

   Purpose

   Level 0 part

   Depositor

   Charlie Gilbert, Nili Ostrov, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pBBR1-UP ORI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   S100L (C299T)

   Promoter

   Available Since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRL271

    Plasmid

    #70254

    Purpose

    sacB plasmid with polylinker; chloramphenicol/erythromycin resistance: use as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.

    Depositor

    Peter Wolk

    Article

    Cai et al J Bacteriol. 1990 Jun;172(6):3138-45.

    Type

    Empty backbone

    Use

    Conditionally lethal

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. G555

    Plasmid

    #216483

    Purpose

    Expression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.

    Depositor

    Christophe Danelon

    Insert

    plsB, plsC, cdsA, pssA
    

    Use

    Cell-free expression

    Tags

    Expression

    Mutation

    Promoter

    T7 for each gene

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pmiRFP709-N1

    Plasmid

    #79989

    Purpose

    Monomeric near-infrared fluorescent protein miRFP709 in mammalian plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    miRFP709
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Fast-GCaMP6f-RS09

    Plasmid

    #67160

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Badura et al Neurophotonics. 2014 Oct;1(2):025008.

    Insert

    Fast-GCaMP6f-RS09
    

    Use

    Tags

    6xHis, T7 tag, and Xpress tag

    Expression

    Bacterial

    Mutation

    L414T within GCaMP6f

    Promoter

    T7

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBAD/HisB-iRFP720

    Plasmid

    #45460

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP720
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

    Promoter

    araBAD

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pXF89

    Plasmid

    #139030

    Purpose

    MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    halotag (no STOP codon, PATC introns)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPAmKate-N1

    Plasmid

    #34587

    Depositor

    Vladislav Verkhusha

    Article

    Gunewardene et al Biophys J. 2011 Sep 21;101(6):1522-8. Ep

    Insert

    PAmKate
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F84W/S148N/S165G compared to mKate (but numbering…

    Promoter

    CMV

    Available Since

    Jan. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-EPN51 (R40A)

    Plasmid

    #80109

    Purpose

    Late1-O3-33-myc-PH(R40A)

    Depositor

    Neil King

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN-51 (R40A)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pmyc-MOV10

    Plasmid

    #10977

    Depositor

    Thomas Tuschl

    Article

    Meister et al Curr Biol. 2005 Dec 6;15(23):2149-55. do

    Insert

    MOV10 (MOV10 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAXY0015

    Plasmid

    #205584

    Purpose

    NpuDnaE(C)_HYG(90-341)_RUBY

    Depositor

    Xiaohan Yang

    Article

    Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

    Insert

    NpuDnaE(C)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available Since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1 NM23-H1

    Plasmid

    #10956

    Depositor

    Patricia Steeg

    Article

    Steeg et al J Natl Cancer Inst. 1988 Apr 6. 80(3):20

    Insert

    NM23 (NME1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge