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We narrowed to 23,602 results for: crispr

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Showing: 11961 - 11980 of 23602 results

  3. pAGT9054

    Plasmid
    #219430
    Purpose
    Transcriptional unit (MoClo-position 2): 2x35Ss_Omega enhancer_PapE-4LF2-N-SpCas9i-N_tOCS
    Depositor
    Tom Schreiber
    Article
    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000
    Insert
    LB_2x35Ss-omega enhancer:PapE-4xLF2-NLS-SpCas9i-NLS:tOCS_RB (Position 2)
    Use
    CRISPR; Moclo compatible level 1 module (position…
    Tags
    PapE-4LF2
    Expression
    Plant
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219430-plasmid-map-sequence-id-441037

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  5. pCAT100

    Plasmid
    #180512
    Purpose
    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180512-plasmid-map-sequence-id-359730

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  6. dCas9-GCN5 FL

    Plasmid
    #179552
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 (KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179552-plasmid-map-sequence-id-354751

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  8. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

    Plasmid
    #86612
    Purpose
    Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via nhej using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86612-plasmid-map-sequence-id-168849

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  9. pAGT9569

    Plasmid
    #219431
    Purpose
    Transcriptional unit (MoClo-position 2): pAtRPS5a_PapE-4LF2-N-SpCas9i-N_tNOS
    Depositor
    Tom Schreiber
    Article
    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000
    Insert
    LB_AtRPS5a:PapE-4xLF2-NLS-SpCas9i-NLS:tNOS_RB (Position 2)
    Use
    CRISPR and Synthetic Biology; Moclo compatible le…
    Tags
    PapE-4LF2
    Expression
    Plant
    Available Since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    219431-plasmid-map-sequence-id-436703

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  13. pCAT527

    Plasmid
    #180514
    Purpose
    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, mNeonGreen, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop-2A-mNeonGreen
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180514-plasmid-map-sequence-id-359727

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  18. pNOC_dfnCas12a-KRAB_crRNA NC

    Plasmid
    #176262
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and spacer sequence is replaced by the type IIS restriction site for endonucleas
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176262-plasmid-map-sequence-id-346962

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Showing: 11961 - 11980 of 23602 results