We narrowed to 209 results for: trypanosoma

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pLew100::NLS-ISceI-HA

Plasmid

#21299

Depositor

Nina Papavasiliou

Article

Boothroyd et al Nature. 2009 May 14;459(7244):278-81. do

Insert

I-SceI (SCEI )

Tags

HA and NLS

Expression

Mammalian

Available Since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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pBES1-LacOx50-blast

Plasmid

#210221

Purpose

Integrates 50xLacO repeats upstream of the BES1 promoter with a blasticidin gene downstream of the promoter.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

50xLacO repeats derived from: PMID: 12864855.

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTrex-Luc-Neo

Plasmid

#48336

Purpose

Trasgenically expresses firefly luciferase in T. cruzi with neomycin selection

Depositor

Rick Tarleton

Article

Sanchez-Valdez et al Elife. 2018 Mar 26;7. pii: 34039. doi: 1

Insert

firely luciferase

Use

Trypanasoma cruzi expression

Promoter

T. cruzi rRNA

Available Since

July 20, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pMOTag-M9-4H

Plasmid

#106379

Purpose

Can be used as a PCR template to amplify the M9-HA point mutant of glmS fragment for gene specific tagging that only allows tagging but not regulation. Acts as a control for glmS based gene regulation

Depositor

Roberto Docampo

Article

Cruz-Bustos et al J Eukaryot Microbiol. 2017 Dec 19. doi:

Insert

GlmS with M9 mutation

Available Since

March 2, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pLew100_myc_BirA*

Plasmid

#41716

Depositor

Graham Warren

Article

Morriswood et al Eukaryot Cell. 2012 Dec 21.

Type

Empty backbone

Use

T. brucei expression

Promoter

PARP

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
pTcINDEX-GW

Plasmid

#234713

Purpose

Used to express any CDS in T. cruzi in a tetracycline regulatable manner. Modified from Taylor et al. (2006) for easy cloning using Gateway recombination cassette.

Depositor

Esteban Serra

Article

Alonso et al Mem Inst Oswaldo Cruz. 2014 Dec;109(8):1

Type

Empty backbone

Use

Trypanosomatid expression

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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TbSIR2RP1

Plasmid

#67242

Purpose

Determine its activity and specificity

Depositor

Cynthia Wolberger

Article

Fahie et al FEBS J. 2009 Dec;276(23):7159-76. doi: 1

Insert

TbSIR2RP1

Tags

MBP, TEV, Thrombin, HIS, and none

Expression

Bacterial

Promoter

p-lac

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only
pBES1-LacOx50-mCh-bla

Plasmid

#210260

Purpose

Integrates 50xLacO repeats and an mCherry gene upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Inserts

50xLacO repeats derived from: PMID: 12864855.
mCherry

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pUC_sgRNA

Plasmid

#68710

Purpose

Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.

Depositor

Roberto Docampo

Article

Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Available Since

Sept. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pSY45

Plasmid

#48355

Purpose

Targets TbMCM-BP for Cre-lox mediated knockout in T. brucei

Depositor

George Cross

Article

Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

Insert

loxP-SAS-HYG-Ty1-TK-loxP flanked by TbMCM-BP-targeting homologies

Use

Cre/Lox; Knockout in t. brucei

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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