Skip to main content
Addgene
Showing: 201 - 240 of 358 results

  4. pDT4

    Plasmid
    #199120
    Purpose
    scrambled promoter circular DNA template
    Depositor
    Jeff Gelles
    Article
    Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12
    Insert
    synthetic transcription template
    Use
    Tags
    Expression
    Bacterial
    Mutation
    mutated 5′-CTGGAGTGCG-3′ to 5′-CTGGAGACCG-3′ to …
    Promoter
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    199120-plasmid-map-sequence-id-388191

    Enlarge

  13. pDT2

    Plasmid
    #199119
    Purpose
    circular transcription template
    Depositor
    Jeff Gelles
    Article
    Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12
    Insert
    lambda PR' - repeat cassette - E. coli rpoB - lambda TR'
    Use
    Tags
    Expression
    Bacterial
    Mutation
    mutated 5′-CTGGAGTGCG-3′ to 5′-CTGGAGACCG-3′ to …
    Promoter
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    199119-plasmid-map-sequence-id-388192

    Enlarge

  16. pWL502-PAM

    Plasmid
    #174382
    Purpose
    Plasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterranei
    Depositor
    Jing Han
    Article
    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:
    Insert
    PAM-spacer
    Use
    CRISPR; Archaeal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    174382-plasmid-map-sequence-id-344136

    Enlarge

  24. pAP05

    Plasmid
    #186261
    Purpose
    sfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoter
    Depositor
    Yongku Cho
    Article
    Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35
    Inserts
    sfGFP
    EL222 (ELI_04755 Erythrobacter litoralis)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    BBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…
    Available since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    186261-plasmid-map-sequence-id-369315

    Enlarge

  26. pAP14

    Plasmid
    #186262
    Purpose
    wgaA (a.k.a expA1) and wgaB (a.k.a expA23), bicistronic, under light light responsive PEL222 promoter and EL222 under constitutively active LacIq promoter
    Depositor
    Yongku Cho
    Article
    Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35
    Inserts
    wgaAB bicistronic
    EL222 (ELI_04755 Erythrobacter litoralis)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    LacIq (CGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCC…
    Available since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    186262-plasmid-map-sequence-id-369317

    Enlarge

  29. tet-pLKO.puro_shHuR CDS

    Plasmid
    #110411
    Purpose
    TRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene, doxycycline inducible, puromycin selection
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAVL1 (HuR)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1/TO (RNA PolIII)
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110411-plasmid-map-sequence-id-213311

    Enlarge

  31. pSCL.175

    Plasmid
    #184996
    Purpose
    Express -Eco1 HEK3 editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: HEK3 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Use
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    HEK3 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184996-plasmid-map-sequence-id-376621

    Enlarge

  32. pSCL.179

    Plasmid
    #185000
    Purpose
    Express -Eco1 HEK4 editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: HEK4 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Use
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    HEK4 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    185000-plasmid-map-sequence-id-376622

    Enlarge

  33. pCfb13220

    Plasmid
    #219895
    Purpose
    The base plasmid of TUNEYALI for TF30
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF30 (YALI1_D10007g) and homologous arm matching TF30
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    219895-plasmid-map-sequence-id-437825

    Enlarge

  34. pCfb13197

    Plasmid
    #219872
    Purpose
    The base plasmid of TUNEYALI forTF07
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF07 ( YALI1_D30097g) and homologous arm matching TF07
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219872-plasmid-map-sequence-id-435636

    Enlarge

  35. pCfb13226

    Plasmid
    #219901
    Purpose
    The base plasmid of TUNEYALI for TF36
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF36 (YALI1_A22330g) and homologous arm matching TF36
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219901-plasmid-map-sequence-id-435635

    Enlarge

  36. pCfb13208

    Plasmid
    #219883
    Purpose
    The base plasmid of TUNEYALI for TF18
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF18 (YALI1_D15792g) and homologous arm matching TF18
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    219883-plasmid-map-sequence-id-435605

    Enlarge

  37. pCfb13195

    Plasmid
    #219870
    Purpose
    The base plasmid of TUNEYALI forTF05
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF05 (YALI1_D02362g) and homologous arm matching TF05
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    219870-plasmid-map-sequence-id-435510

    Enlarge

  38. pCfb13229

    Plasmid
    #219904
    Purpose
    The base plasmid of TUNEYALI for TF39
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF39 (YALI1_B16808g) and homologous arm matching TF39
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    219904-plasmid-map-sequence-id-435474

    Enlarge

  39. pCfb13192

    Plasmid
    #219867
    Purpose
    The base plasmid of TUNEYALI for TF02
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF02 (YALI1_D21702g) and homologous arm matching TF02
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    219867-plasmid-map-sequence-id-435432

    Enlarge

  40. pCfb13214

    Plasmid
    #219889
    Purpose
    The base plasmid of TUNEYALI for TF24
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF24 (YALI1_F28995g) and homologous arm matching TF24
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    219889-plasmid-map-sequence-id-435424

    Enlarge

Showing: 201 - 240 of 358 results