Skip to main content
Addgene
Showing: 201 - 240 of 420 results

  1. pH-STEME-1-esgRNA

    Plasmid
    #138135
    Purpose
    Targeted simultaneous C-to-T and A-to-G in rice
    Depositor
    Caixia Gao
    Insert
    APOBEC3A-wtTadA-TadA7.10-nCas9-UGI-NLS
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138135-plasmid-map-sequence-id-311741

    Enlarge

  2. pH-STEME-NG-esgRNA

    Plasmid
    #138136
    Purpose
    Targeted simultaneous C-to-T and A-to-G in rice
    Depositor
    Caixia Gao
    Insert
    APOBEC3A-wtTadA-TadA7.10-nCas9-NG-UGI-NLS
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138136-plasmid-map-sequence-id-311723

    Enlarge

  3. pEGB3_alpha1 P35Sx2_GFP_Tnos (GB2980)

    Plasmid
    #160634
    Purpose
    TU for wtGFP expression driven by 2x35s promoter.
    Depositor
    Diego Orzaez
    Insert
    GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    2x35s
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160634-plasmid-map-sequence-id-321964

    Enlarge

  4. pDGB3 alpha1 P35S:BFP:T35S (GB3416)

    Plasmid
    #160637
    Purpose
    TU for the constitutive expression of Blue Fluorescent Protein
    Depositor
    Diego Orzaez
    Insert
    BFP
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    35S
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160637-plasmid-map-sequence-id-321961

    Enlarge

  5. pEGB3_alpha1 Pnos:P19:Tnos (GB2762)

    Plasmid
    #160632
    Purpose
    TU for expression of P19 driven by Nos promoter.
    Depositor
    Diego Orzaez
    Insert
    P19
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos
    Available since
    Jan. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    160632-plasmid-map-sequence-id-321966

    Enlarge

  6. AtS/MAR10 insulator sequence (GB3459)

    Plasmid
    #160639
    Purpose
    pEGB3a2 AtS/MAR10
    Depositor
    Diego Orzaez
    Insert
    AtS/MAR10 insulator sequence
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Jan. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    160639-plasmid-map-sequence-id-321955

    Enlarge

  7. pDGB3 alpha2 AtUBQ10:LbCas12a:Tnos (GB2479)

    Plasmid
    #160629
    Purpose
    TU for the expression of LbCas12a
    Depositor
    Diego Orzaez
    Insert
    AtUBQ10-:LbCpf1(Cas12a):Tnos
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    UBQ10
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160629-plasmid-map-sequence-id-321990

    Enlarge

  12. pEGB2_alpha2 P35S:CUP2:GAL4AD:T35S (GB1039)

    Plasmid
    #160607
    Purpose
    TU for the constitutive expression of the transcriptional activator protein CUP2 in C-terminal fusion with GAL4 activation domain (GAL4AD). Binds to specific DNA operator in the presence of copper ions and promotes transcription.
    Depositor
    Diego Orzaez
    Insert
    CUP2Gal4AD
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    P35S
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160607-plasmid-map-sequence-id-322043

    Enlarge

  13. pDGB3 alpha1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1656)

    Plasmid
    #160618
    Purpose
    Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).
    Depositor
    Diego Orzaez
    Insert
    Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
    Use
    CRISPR
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos, 35S
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160618-plasmid-map-sequence-id-322016

    Enlarge

  14. pEGB3_alpha1 gRNAs 2.1 P35S -104 -162 -229 (GB2759)

    Plasmid
    #160631
    Purpose
    Multiplex construct with 2.1 aptamer in scf to target -104 -162 -229 positions of 35s promoter.
    Depositor
    Diego Orzaez
    Insert
    gRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    U626
    Available since
    Jan. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    160631-plasmid-map-sequence-id-321967

    Enlarge

  15. pDGB3 omega1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1657)

    Plasmid
    #160640
    Purpose
    Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).
    Depositor
    Diego Orzaez
    Insert
    Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
    Use
    CRISPR
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos, 35S
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    160640-plasmid-map-sequence-id-321670

    Enlarge

  17. pEGB3 omega1 Tnos:BASTA:Pnos - P35S:hCas9:Tnos (GB3469)

    Plasmid
    #160647
    Purpose
    module containing the human codon optimized Cas9 and the BASTA selection marker
    Depositor
    Diego Orzaez
    Insert
    BASTA / Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos, P35S
    Available since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160647-plasmid-map-sequence-id-319125

    Enlarge

  19. pEGB3_alpha2 35:PhyB:VP16 (GB2745)

    Plasmid
    #160630
    Purpose
    TU for the expression of chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch
    Depositor
    Diego Orzaez
    Insert
    PhyBVP16
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    35S
    Available since
    Jan. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    160630-plasmid-map-sequence-id-321969

    Enlarge

  20. pFGL815

    Plasmid
    #52322
    Purpose
    A vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 69-bp MCS (multiple cloning site) which contains 12 unique restriction sites.
    Depositor
    Naweed Naqvi
    Article
    Yang et al Fungal Genet Biol. 2014 Apr 24. pii: S10
    Type
    Empty backbone
    Use
    Agrobacterium
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52322-plasmid-map-sequence-id-81025

    Enlarge

  21. pLSLR

    Plasmid
    #51500
    Purpose
    Single component plant T-DNA plasmid. Contains cis- and trans-acting replicational elements from the bean yellow dwarf virus in an LIR-SIR-Rep/RepA-LIR orientation.
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Type
    Empty backbone
    Use
    Plant t-dna plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51500-plasmid-map-sequence-id-78614

    Enlarge

  22. pLSLGFP

    Plasmid
    #51494
    Purpose
    pLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmid
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Inserts
    GFP
    us:NPTII repair template
    Use
    Plant t-dna plasmid
    Tags
    NLS and flag
    Expression
    Mutation
    Promoter
    virion-sense LIR and 2x35S
    Available since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51494-plasmid-map-sequence-id-78590

    Enlarge

  23. pIF4

    Plasmid
    #196069
    Purpose
    Expresses dCas9-Mxi1 with MoRP27 promoter and express gRNA with MoU6 promtoer in M. oryzae
    Depositor
    Kabin Xie
    Article
    Zhang et al mSphere. 2023 Feb 21;8(1):e0059422. doi:
    Insert
    dCas9-Mxi1
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    MoRP27 promoter
    Available since
    April 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    196069-plasmid-map-sequence-id-389524

    Enlarge

  25. pIF3

    Plasmid
    #196068
    Purpose
    Expresses dCas9-Mxi1 with MoRP27 promoter and express gRNA with MoU3 promtoer in M. oryzae
    Depositor
    Kabin Xie
    Article
    Zhang et al mSphere. 2023 Feb 21;8(1):e0059422. doi:
    Insert
    dCas9-Mxi1
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    MoRP27 promoter
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    196068-plasmid-map-sequence-id-385304

    Enlarge

  29. pGKO1

    Plasmid
    #63611
    Purpose
    a binary Agrobacterium vector carrying the HSVtk gene as a negative selection marker against ectopic transformants.
    Depositor
    Seogchan Kang
    Article
    Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92
    Type
    Empty backbone
    Use
    Binary fungal vector
    Tags
    Expression
    Mutation
    Promoter
    Lac Promoter
    Available since
    July 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    63611-plasmid-map-sequence-id-119638

    Enlarge

  39. pLSLGUS

    Plasmid
    #51517
    Purpose
    pLSL with GUS coding sequence followed by 3 kb of filler sequence Rep is not included on this plasmid
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Inserts
    GUS
    3kb filler sequence
    Use
    Plant t-dna plasmid
    Tags
    NLS and flag
    Expression
    Mutation
    Promoter
    virion-sense LIR and 2x35S
    Available since
    May 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51517-plasmid-map-sequence-id-78696

    Enlarge

  40. pFGL815N

    Plasmid
    #52323
    Purpose
    A vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 68bp MCS (multiple cloning site) which contains 12 unique restriction sites.
    Depositor
    Naweed Naqvi
    Article
    Yang et al Fungal Genet Biol. 2014 Apr 24. pii: S10
    Type
    Empty backbone
    Use
    Agrobacterium
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    52323-plasmid-map-sequence-id-81026

    Enlarge

Showing: 201 - 240 of 420 results